ACL_RS00040

Uniprot: A9NE70
Description: DNA gyrase subunit A
EC number: 5.99.1.3
Annotation score: 3 out of 5
Miscellaneous [CC]: MISCELLANEOUS: Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes. {ECO:0000256|HAMAP-Rule:MF_01897}.
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000256|HAMAP-Rule:MF_01897, ECO:0000256|SAAS:SAAS00075911}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. {ECO:0000256|HAMAP-Rule:MF_01897}.
Pathway:
Active site: ACT_SITE 126 126 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000256|HAMAP-Rule:MF_01897}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gyrA
Gene names (synonym):
Mass: 97,542
Subunit structure [CC]: SUBUNIT: Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis. {ECO:0000256|HAMAP-Rule:MF_01897}.
Gene ontology (GO): chromosome [GO:0005694]; cytoplasm [GO:0005737]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (ATP-hydrolyzing) activity [GO:0003918]; DNA topological change [GO:0006265]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0003677; GO:0003918; GO:0005524; GO:0005694; GO:0005737; GO:0006261; GO:0006265
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the topoisomerase GyrA/ParC subunit family. {ECO:0000256|HAMAP-Rule:MF_01897}.; SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. {ECO:0000256|SAAS:SAAS00565436}.
Protein families: Topoisomerase GyrA/ParC subunit family; Type II topoisomerase GyrA/ParC subunit family
Coiled coil:
Domain [FT]: DOMAIN 15 467 TOP4c. {ECO:0000259|SMART:SM00434}.
Motif: MOTIF 529 535 GyrA-box. {ECO:0000256|HAMAP-Rule:MF_01897}.
Region:
EMBL: CP000896
ProteinModelPortal: A9NE70
MEROPS:
EnsemblBacteria KO: ABX80650
UniPathway: K02469
CDD:
Gene3D:
HAMAP: 3.30.1360.40;3.90.199.10
InterPro: MF_01897
PANTHER: IPR024946;IPR005743;IPR006691;IPR013760;IPR002205;IPR013758
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF03989;PF00521
SMART:
SUPFAM: SM00434
TIGRFAMs: SSF56719

7654-10258(+)

>nucleotide sequence
ATGAGTGAAAATACAGATAAACTACATGATAAGATTAAAGAAGTAAACATTACTTCAGAG
ATGAAAACATCCTTCTTAAACTATGCGATGAGTGTTATTGTATCTCGTGCACTTCCAAAT
GCTAAAGACGGATTAAAACCAGTTCAAAGACGTATTCTTTACGGTATGCATGAAAATAAC
ATGTATGCAAATACGGCACATAAAAAATCAGCGAGAATCGTTGGGGATGTTATGGGGAAA
TATCACCCTCATGGTGACTCTTCAATTTATGATGCCATGGTGCGTATGGCACAACCATTT
AGTTATAGAAAACCATTAGTTGACGGTCATGGTAACTTTGGTTCTGTTGACGGTGATGGT
GCTGCTGCGATGCGTTATACCGAAGCTAGACTATCTAAGATAGCTGGTGAAATGGTTAGA
GATATCGACAAAAACACAGTAAATTTTGTAGATAACTATGATGGTTCAGAACGTGAACCA
CAAGTACTACCATCAAGATATCCAAACTTACTAGTAAACGGTTCAACCGGTATTGCTGTT
GGTATGGCTACAAATATTCCACCACATAATTTAGGTGAAGTGATTGATGCAATCTTTGCA
TATTTAGATAATCCTGATATTACAGTACTTGAATTAATGCAATACATTAAGGGACCAGAC
TTCCCAACAGGTGGTCAAATTCTTGGTATTACAGGTCTTCGTCAAGCATATGAAACAGGT
AAAGGTATTATTGCTGTTCGTGCACAACATGAAATTGTTGAAACTAAAAATAGAACAGAA
TTAATCATTACTGAAATACCTTACCAAGTAAATAAAACAACATTAATTGAAAGAATCGCT
GAAGTTGTTAAAAATAAGATCATCGAAGGTATTTCAGACTTAAGAGATGAATCCAATAGA
AAAGGTATGCGTATCGTTATTGAACTTAGAAAAGATACTAATCCATCTGTTACCTTAAAT
AACTTATATAAACATACTCAACTTCAAACATCCTTTGGTATTAATATGATTGCTTTAGTC
GATGGACAACCAAGACTACTTAGTTTAAAAGATGCATTAACACATTATATTGCACACCAA
ATTGAAATTATTACACGTCGTACACAATTTGATTTAGAAAAAGCTGAAGCTAGAATGCAT
ATATTAGAAGCACTTGTTAAAGCTTTAAATGATGTAGATAATGTCATTAAAATCATCAAA
GATGCAAAAAATCCTGAAGATGCTAGAAATAATTTAATAGCAACTTATGATTTCACAGAA
ATTCAAGCTCGTTCTATTTTAACGATGCAACTTCAAAGATTAACAGGTCTTGAAATCGAT
AAAATTCATGAAGAAGCAGATAATTTAGCACATAAAATTACTGAATACAAACAAATTTTA
GGTGATGAAGATCTAAAAATCGAAATCATAAAGTCTGAACTTCAAGAAATTAAAGATAAA
TATGATGATCCTCGTATGTCAGAAGTGAATTTATATGAAGATCTTGATATTGATAATGAA
GATTTAATCCCTGTTGAAGATATCGTTGTAACTGTAACAAATAATGGTTACATCAAACGT
ATGAATTTAGATGAGTATAAATTACAAAACAGAGGTGGCGTCGGCATGAGTGGTGTTAAA
CTACATGACGATGACTTTGTTGAGCATATTGCGATGACATCAACACATGACTTCCACTTA
TTCTTTACCAACCTAGGTCGAGTATTTAAACTTAAAGGGTATACCTTACCATCTGGATCA
CGTCAATCTAAAGGGGTACCAATTGTAAACTACCTAAACTTCCAACCAGGAGAAAAATTA
GCAAGCTTTACAACCATTAAAGACTTTGATGCTGAAGATCACTATCTATTCTTTGTTACA
AAGAGGGGTATCGTAAAACGTACACATATTAATAACTACAAGAATATTAGACAAAACGGT
ATTATAGCAATCAGTTTAAGGGAAGATGATGAACTCTTAGTGACGAAATCAACCAATGGT
CATAAACATATCTTACTAGGTGCATCAAACGGTAAAGCTATCCACTTCCTTGAAACAGAT
ATTAGAGAAATTGGACGTACAGCAACCGGTGTCCGTGGTATGGACATACCAGATGATGAA
GAAATTGTTGGATTTGCAGTTGTAGACGGTATTGAAGACGATATCTTAGTCATTACCGAA
AACGGCTATGGAAAGCGTACATCGATTACTGAATATAGACTTCAAAACAGAGGCGGTAAA
GGTGTTAAGACATTAAATGTAACAGATAAAAACGGTAAGTTAAAAACTTTACGTAAAGTT
ACAAACGATGAAGACATCATTGTAGTAAGTGATAGAGGTATTACAATACGTATGCCAATT
GATCAAATCTCACAAACAAAACGTGCAACTCAAGGTGTAAGAATTATTACATTAAAAGGT
GATCAAAAAGTATCAACAATCGCGATTGTAGATCATCAGGAACCTGAAGAAGAAGTTGTA
TCTAATGAAGCTGTAGTAACTCAAGAACAAATGGCTCTAAGCAATCAAAAAAAGGTCTTA
GAAGTTGTTGAACTTGAAGCACTAGAAGAATCAGTAGATGAAGAAGATACAAAAAACGCC
AAACAAACTAAATTAGATTTATAA

>protein sequence
MSENTDKLHDKIKEVNITSEMKTSFLNYAMSVIVSRALPNAKDGLKPVQRRILYGMHENN
MYANTAHKKSARIVGDVMGKYHPHGDSSIYDAMVRMAQPFSYRKPLVDGHGNFGSVDGDG
AAAMRYTEARLSKIAGEMVRDIDKNTVNFVDNYDGSEREPQVLPSRYPNLLVNGSTGIAV
GMATNIPPHNLGEVIDAIFAYLDNPDITVLELMQYIKGPDFPTGGQILGITGLRQAYETG
KGIIAVRAQHEIVETKNRTELIITEIPYQVNKTTLIERIAEVVKNKIIEGISDLRDESNR
KGMRIVIELRKDTNPSVTLNNLYKHTQLQTSFGINMIALVDGQPRLLSLKDALTHYIAHQ
IEIITRRTQFDLEKAEARMHILEALVKALNDVDNVIKIIKDAKNPEDARNNLIATYDFTE
IQARSILTMQLQRLTGLEIDKIHEEADNLAHKITEYKQILGDEDLKIEIIKSELQEIKDK
YDDPRMSEVNLYEDLDIDNEDLIPVEDIVVTVTNNGYIKRMNLDEYKLQNRGGVGMSGVK
LHDDDFVEHIAMTSTHDFHLFFTNLGRVFKLKGYTLPSGSRQSKGVPIVNYLNFQPGEKL
ASFTTIKDFDAEDHYLFFVTKRGIVKRTHINNYKNIRQNGIIAISLREDDELLVTKSTNG
HKHILLGASNGKAIHFLETDIREIGRTATGVRGMDIPDDEEIVGFAVVDGIEDDILVITE
NGYGKRTSITEYRLQNRGGKGVKTLNVTDKNGKLKTLRKVTNDEDIIVVSDRGITIRMPI
DQISQTKRATQGVRIITLKGDQKVSTIAIVDHQEPEEEVVSNEAVVTQEQMALSNQKKVL
EVVELEALEESVDEEDTKNAKQTKLDL