ACL_RS00050

Uniprot: A9NE72
Description: serine--tRNA ligase
EC number: 6.1.1.11
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). {ECO:0000255|HAMAP-Rule:MF_00176}.; CATALYTIC ACTIVITY: ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). {ECO:0000255|HAMAP-Rule:MF_00176}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). {ECO:0000255|HAMAP-Rule:MF_00176}.
Pathway: PATHWAY: Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00176}.
Active site:
Binding site: BINDING 285 285 Serine. {ECO:0000255|HAMAP-Rule:MF_00176}.; BINDING 385 385 Serine. {ECO:0000255|HAMAP-Rule:MF_00176}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 262 264 ATP. {ECO:0000255|HAMAP-Rule:MF_00176}.; NP_BIND 349 352 ATP. {ECO:0000255|HAMAP-Rule:MF_00176}.
Site:
Gene names (primary): serS
Gene names (synonym):
Mass: 48,263
Subunit structure [CC]: SUBUNIT: Homodimer. The tRNA molecule binds across the dimer. {ECO:0000255|HAMAP-Rule:MF_00176}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; serine-tRNA ligase activity [GO:0004828]; selenocysteine biosynthetic process [GO:0016260]; selenocysteinyl-tRNA(Sec) biosynthetic process [GO:0097056]; seryl-tRNA aminoacylation [GO:0006434]
Gene ontology IDs: GO:0004828; GO:0005524; GO:0005737; GO:0006434; GO:0016260; GO:0097056
Chain: CHAIN 1 423 Serine--tRNA ligase. /FTId=PRO_1000077184.
Signal peptide:
Domain [CC]: DOMAIN: Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding. {ECO:0000255|HAMAP-Rule:MF_00176}.
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. {ECO:0000255|HAMAP-Rule:MF_00176}.
Protein families: Class-II aminoacyl-tRNA synthetase family, Type-1 seryl-tRNA synthetase subfamily
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 231 233 Serine binding. {ECO:0000255|HAMAP-Rule:MF_00176}.
EMBL: CP000896
ProteinModelPortal: A9NE72
MEROPS:
EnsemblBacteria KO: ABX80652
UniPathway: K01875
CDD:
Gene3D: cd00770
HAMAP: 1.10.287.40
InterPro: MF_00176
PANTHER: IPR002314;IPR006195;IPR002317;IPR015866;IPR033729;IPR010978
PIRSF: PTHR11778
PRINTS: PIRSF001529
PROSITE: PR00981
Pfam: PS50862
ProDom: PF02403;PF00587
SMART:
SUPFAM:
TIGRFAMs: SSF46589

13186-14458(+)

>nucleotide sequence
ATGTTAGATTTAAAATATATAACTGAAAATATAGATGAAGTCATCTTGAAGTTAAATACT
AGAGGTGGTGATTTTAGCCACTTAAGACAATTAATAGATTTACAAGAAGAGCGTAAATCT
GTAATTAAAGAAGTTGAAGACTTAAAAGCAAAAAGAAACGAATACTCCAAAGAAATTGGG
GAACTTAAAAGACAAAAACAAGATGCTTCTCACGTGTTATTAAAAGTAGAGTCCATTAAA
AGTGACATACCAGCACTTGAGTTAAAACTTGGTGAGATTGATGAAAAAATTAATAAAGAA
CTCATCGTTTTACCAAATATTCCTGCTGACGATGTACCGGTTGGTAAAGATGAATCTGCA
AATATTGAAATTAAAAAATGGGGTACTATTAGACATTTTGATTTTGAAGTAAAAGATCAT
ACTCAATTAGGTGAAGCACTAAATATATTAGACTTTGAACGTGCAACTAAAATCACTGGT
CCACGTTTTGTAGTAGATAAAGGGCTAGGTGCTAGACTGGAAAGAGCGCTTATAAACTTT
ATGATAGATACACATGCGTATACACATGGTTATACCGAAATCATTCCACCGTTTATTGTA
AATGATAAATCTATGTATGCAACAGGACAATTTCCTAAGTTTAAAGAAGATGCTTTTAAA
TTAGAAGGATTTGATTGGTATTTAAATCCAACAGCAGAAGTACCAACTATTAACCTATTT
AGAGATGAAATCATTGATAATGATGCATTACCAATTCAGTATGTAGCTTATACAACAGCA
TTTAGATCAGAGGCAGGTTCTGCTGGACGAGATACTAAGGGTATTTTAAGACAACATCAA
TTCAATAAAGTTGAATTAATTAAATTTGCACGACCTGAAGATTCTGAACAAGCTCATCAA
GATATGTTAGCAAACTCAGAAAGAATACTACAACTATTAAATATTCCTTATCGAGTAGTT
GTCTTATCAACAGGAGACATGGGCTTTGGTATGAGTAAGACATACGATATTGAAGTATGG
TTACCAGGTCAAAATATGTACCGTGAAATTGGATCTATTTCAAATGCTAGAGACTTTCAA
GCAAGAAGAGCCAACATTCGCTTTAAACGTTCAAAAGATGCTAAAACAGAATATGTGCAT
ACATTAAACGGTTCTGGTTTAGCTGTTGGTAGAACAATGATTGCAGTACTAGAGAACTAT
CAAAATCAAGATGGATCAATTACAATACCAGAAGTCTTAAAACCATATATGGGTGTAGAA
GTTATTAAATAA

>protein sequence
MLDLKYITENIDEVILKLNTRGGDFSHLRQLIDLQEERKSVIKEVEDLKAKRNEYSKEIG
ELKRQKQDASHVLLKVESIKSDIPALELKLGEIDEKINKELIVLPNIPADDVPVGKDESA
NIEIKKWGTIRHFDFEVKDHTQLGEALNILDFERATKITGPRFVVDKGLGARLERALINF
MIDTHAYTHGYTEIIPPFIVNDKSMYATGQFPKFKEDAFKLEGFDWYLNPTAEVPTINLF
RDEIIDNDALPIQYVAYTTAFRSEAGSAGRDTKGILRQHQFNKVELIKFARPEDSEQAHQ
DMLANSERILQLLNIPYRVVVLSTGDMGFGMSKTYDIEVWLPGQNMYREIGSISNARDFQ
ARRANIRFKRSKDAKTEYVHTLNGSGLAVGRTMIAVLENYQNQDGSITIPEVLKPYMGVE
VIK