ACL_RS00095

Uniprot: A9NE81
Description: ribose-phosphate pyrophosphokinase
EC number: 2.7.6.1
Annotation score: 4 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. {ECO:0000256|HAMAP-Rule:MF_00583}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00583}; ; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00583}
Enzyme regulation:
Function [CC]: FUNCTION: Involved in the biosynthesis of ribose 1,5-bisphosphate. Catalyzes the transfer of pyrophosphoryl group from ATP to ribose-5-phosphate to yield phosphoribosyl diphosphate (PRPP) and AMP. {ECO:0000256|HAMAP-Rule:MF_00583}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. {ECO:0000256|HAMAP-Rule:MF_00583}.
Active site:
Binding site: BINDING 108 108 Ribose-5-phosphate. {ECO:0000256|HAMAP-Rule:MF_00583}.; BINDING 134 134 ATP. {ECO:0000256|HAMAP-Rule:MF_00583}.; BINDING 139 139 ATP. {ECO:0000256|HAMAP-Rule:MF_00583}.; BINDING 173 173 Ribose-5-phosphate. {ECO:0000256|HAMAP-Rule:MF_00583}.
Calcium binding:
DNA binding:
Metal binding: METAL 132 132 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00583}.; METAL 134 134 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00583}.; METAL 143 143 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00583}.; METAL 147 147 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00583}.
Nucleotide binding: NP_BIND 41 43 ATP. {ECO:0000256|HAMAP-Rule:MF_00583}.; NP_BIND 100 103 ATP. {ECO:0000256|HAMAP-Rule:MF_00583}.
Site:
Gene names (primary): prsA
Gene names (synonym): prs
Mass: 34,915
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; kinase activity [GO:0016301]; magnesium ion binding [GO:0000287]; ribose phosphate diphosphokinase activity [GO:0004749]; 5-phosphoribose 1-diphosphate biosynthetic process [GO:0006015]; nucleotide biosynthetic process [GO:0009165]; ribonucleoside monophosphate biosynthetic process [GO:0009156]
Gene ontology IDs: GO:0000287; GO:0004749; GO:0005524; GO:0005737; GO:0006015; GO:0009156; GO:0009165; GO:0016301
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the ribose-phosphate pyrophosphokinase family. {ECO:0000256|HAMAP-Rule:MF_00583}.
Protein families: Ribose-phosphate pyrophosphokinase family
Coiled coil:
Domain [FT]: DOMAIN 9 124 Pribosyltran_N. {ECO:0000259|Pfam:PF13793}.
Motif:
Region: REGION 196 198 Ribose-5-phosphate binding. {ECO:0000256|HAMAP-Rule:MF_00583}.; REGION 223 230 Ribose-5-phosphate binding. {ECO:0000256|HAMAP-Rule:MF_00583}.
EMBL: CP000896
ProteinModelPortal: A9NE81
MEROPS:
EnsemblBacteria KO: ABX80661
UniPathway: K00948
CDD:
Gene3D: cd06223
HAMAP: 3.40.50.2020
InterPro: MF_00583_B
PANTHER: IPR000842;IPR029099;IPR000836;IPR029057;IPR005946
PIRSF:
PRINTS:
PROSITE:
Pfam: PS00114
ProDom: PF14572;PF13793
SMART:
SUPFAM:
TIGRFAMs: SSF53271

20971-21937(+)

>nucleotide sequence
ATGACTATAGATGAAAAAAAGGCTAAATTATTTACACTTTCCGCTAATAAACCGCTAGCT
GAAAAAATAGCTAAAAGTGCAGGAATTCCATTATCAAATGTTGAAGTTATTCGATTTGCT
GATGGAGAGATTACCGTTAACATTGAAGAATCTGTAAGAGGTAACCATGTTTTTGTGATT
CAACCAACATCAGAACCAGCAAATGACCATTTAATGGAGGTATTGGTATTAACGGATGCA
CTTAAGAGAGCATCTGCAGCATCTATTACAATCATCATGCCATATTTTGGTTATTCAAGA
CAAGATAGAAAAGTTAAGTCACGTCAACCAATTACCGCTAAATTAGTAGCTAACCTACTG
ACTGTAGCAGGTGTTGATCGTGTCGTAAGTATTGACTTACACGCGGCTCAAATTCAAGGT
TTCTTTGATATTCCAATTGACAATTTCCCTGCTGCACCAACACTAGCTTCATATTTTAGA
AGAAAAAAATTAGAAAACGTTGTTGTAGTATCTCCTGACCATGGTGGTGTGACACGTGCA
CGTGTATTTGCATCATTTTTTAATGCCCCACTTGCAATCATAGATAAGAGAAGACCTGAA
CCTAATAAGGCAGAGGTTATGAATATCATTGGTGATGTAAAAGGTGCAACATGTATCATG
ATTGATGACATCATAGATACTGGTGGTACATTAATGGCTGGAGCAAATGCTTTAAAAGAA
GCAGGTGCTAAAGAAGTATATGCAGCAGCAACACATGGTGTATTAACTTCTAATGCAACA
GAAAGATTACAAAACTCAGTGATTAATGAGATTGTTATTACAGATACAATCTACTTAGAT
CCAGCTAAAAATCAACCAAAACTTAAACAATTATCTATTGGTGCACTGTTAGGTGAAGCA
ATCATCCATATTTTACAAGACGAACCAATCTCTCAAATCTTTAATAGAATTCAAGAGGAT
CAATAA

>protein sequence
MTIDEKKAKLFTLSANKPLAEKIAKSAGIPLSNVEVIRFADGEITVNIEESVRGNHVFVI
QPTSEPANDHLMEVLVLTDALKRASAASITIIMPYFGYSRQDRKVKSRQPITAKLVANLL
TVAGVDRVVSIDLHAAQIQGFFDIPIDNFPAAPTLASYFRRKKLENVVVVSPDHGGVTRA
RVFASFFNAPLAIIDKRRPEPNKAEVMNIIGDVKGATCIMIDDIIDTGGTLMAGANALKE
AGAKEVYAAATHGVLTSNATERLQNSVINEIVITDTIYLDPAKNQPKLKQLSIGALLGEA
IIHILQDEPISQIFNRIQEDQ