ACL_RS00105

Uniprot: A9NE83
Description: transcription-repair coupling factor
EC number: 3.6.4.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site. {ECO:0000256|HAMAP-Rule:MF_00969}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 622 629 ATP. {ECO:0000256|HAMAP-Rule:MF_00969}.
Site:
Gene names (primary): mfd
Gene names (synonym):
Mass: 132,313
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; damaged DNA binding [GO:0003684]; helicase activity [GO:0004386]; regulation of transcription, DNA-templated [GO:0006355]; transcription-coupled nucleotide-excision repair, DNA damage recognition [GO:0000716]
Gene ontology IDs: GO:0000716; GO:0003684; GO:0004386; GO:0005524; GO:0005737; GO:0006355
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains 1 helicase ATP-binding domain. {ECO:0000256|HAMAP-Rule:MF_00969}.; SIMILARITY: Contains 1 helicase C-terminal domain. {ECO:0000256|HAMAP-Rule:MF_00969}.; SIMILARITY: In the C-terminal section; belongs to the helicase family. RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.; SIMILARITY: In the N-terminal section; belongs to the UvrB family. {ECO:0000256|HAMAP-Rule:MF_00969}.
Protein families: Helicase family, RecG subfamily; UvrB family
Coiled coil: COILED 541 568 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 609 770 Helicase ATP-binding. {ECO:0000259|PROSITE:PS51192}.; DOMAIN 791 945 Helicase C-terminal. {ECO:0000259|PROSITE:PS51194}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE83
MEROPS:
EnsemblBacteria KO: ABX80663
UniPathway: K03723
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_00969
PANTHER: IPR003711;IPR011545;IPR014001;IPR001650;IPR004576;IPR027417;IPR005118
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51192;PS51194
ProDom: PF02559;PF00270;PF00271;PF03461
SMART:
SUPFAM: SM01058;SM00487;SM00490;SM00982
TIGRFAMs: SSF141259;SSF52540

22547-25979(+)

>nucleotide sequence
ATGACTAAAAATTTATTAAACTCCATATATAAAGATGCCGCCATTTTAAACGCCAGAACA
GGTGATTTTAAAGGTGTATCCCATAGCTATCTACAGACAATGCTTTCGCTTAGATATGAT
ATTTCTAATAAAAATATTTTTGTAGTTTTACCAAATCTATATGAGGCACAAAAATACTAT
GATACGTTATCTTCTATTATTGATGAAAGTAAAGTATTGTTTTATCCAATGGATCAAACA
CTAACCTCCATGATGGCGTTAGGGTCTCCTGAGTTTAAAAACGAGCGTCTATTTACTTTA
AGAAAACTTTTACAAGCAGATGATAAGTATATCATCATCACCACTCAAGAAGGTATTTTA
CACAGACAATTAAAGCCTGAAGATTATGAGCGTAGTGTTAAAAAAATAAGTGTTAATCAA
GATTACGATCTTACTGACCTTACAAAAAAACTAATATATGATGGCTATCAGTTTAACTAT
ACAGTAGAACGACCAGGCGAGTTTTCTATTAGAGGTTCTATATTAGATATCTATACACAT
GATTATAAAGATCCTTACCGTCTAGACTTTTTTGGTGATACCTTAGAATCAATAAAGACA
TTTGATGTACAAACTCAAAAAAGTATGAATCATATTACATCCATAGACATTGCCCCATTA
AATGAATTATTTTATACGGATGAATTAAAAGAACAGGCACTTGAAAAAATAAGTAAATAT
TTTAGTGGCTTTAAATTATCTGAAAAAGAAGATAAAAAGTTAGAAACTGATCTTATGCAT
ATTGAAGAACGTAAACGTATGGATAGTCTATCTATGTATATAGAGTTCTTTAATGATGAA
GTGACAACCATTTTAGATTTTAGTAACCCATATGACTTAATACTTGTTGATAGTTATAAG
ATGGAACTGAATGAAGAAACAACGTATCAAGATTTATTAACCTATAGTCATACCATGCAA
GGAGAGGCATTCATAGGTTTAAACTTTAAAGTAAAATTAAAAGAACTACTGAAGAAAAAT
CATTTATCATTTCAAATATATTCAGTATCTGATAAACCATCTACTGATTTAGGTGTTTTA
GATGTGGATCCATTTGTAGGACATTTAGAACAACTGATCTTATTTATAAATGAATATAAC
GGATTTAATATACTCATATCTACTAAGACTATGGTATCTTTTGAACGTATTAAAGAGCAT
TTAACGAATCACAAGATAAGTTATAGTGTCAACCAGTTTAAAGACGCAATGGTTGTATTA
GTGGAAGAAGATTTACCGGGATCATTTATTGATCTAAATCATAAATTTATCATCTTAACT
GAAGATACTATCTTAGATACTAAGAAAAAAGCTAAGGTCAGATACCGCTCAGTTATTAAC
CAAGCCGTAAAAATTAGAGATATCTCAGAACTACAAAATGGTGATTATGTTGTGCACTAT
GATTTTGGTATTGGTCAATACATTGGTCTAAAGACAATGACTTTATCTGGAGATAAAAGA
GATTATCTACATATTGTTTATGATAAAGATGAGGCACTTTATGTACCAACAGATCAAATT
GATTTAGTTTTAAAATATAAAAGTTATGATCAAGTTAAACCTAAACTATCTAAGTTAAAC
GCTAAACAATGGAGTAAAACCAAAGCTCAAGTCAAACAAAAGATTAAAGACTTATCGGAT
AGACTACTCAATCTTTATGCCAAAAGACATCAAGCAGAAGGATATCAGTTTAGTCCTAGA
ACAGATATGTTAACTTCTTTTGAAAAAGACTTTAATTATGATGAAACCATTGACCAACAA
AAAGCGATTGATGCCGTATTTGAAGATATGGAAAGTTCTAAACCTATGGATAGACTTATT
GCTGGGGATGTTGGTTATGGTAAAACAGAAGTGGCACTTCGTGCAGCCTTTAAAGCTGTT
GTAGATGCCAAACAAGTTGCTTACCTAGTACCGACTACGGTACTGGCTAGACAACACTAT
TTAACCTTTAAAGACAGGTTTGAAAAGTATGGTGGAAGTGTTGCACTACTTTCTAGATAT
GTTAGTAAAAGGGAACAAAAAGAAGTGCTAGAAAAAATAGCTAAGGGTTATATTGATGTC
GTTATCGGAACACACAGATTATTAAGTGATGACATTGTCTTTAAAGATTTAGGTTTATTT
ATTATCGATGAGGAACAACGCTTTGGGGTTCAACATAAAGAAAAAATTAAAGAAATTAAA
GTGAATGTGGATACCCTAACTTTATCTGCAACACCTATTCCAAGAACGCTTCAAATGGCC
ATGTACGGGCTTAAGGACCTATCTATGATTGATACACCGCCTTTAAATAGATATCCCGTA
CAAACCTATGTTGTTGAAAGACAACCTGCATTAATTAAAGAAGCTATTGATAGAGAACTA
TCACGAGGTGGACAAGTATTTTATTTATTTAATAATACAGAGCGCATGGAAGCTCAAGTA
TTAAAACTACAACAACTTGTACCAAATGCAAGAATTACATATGCACATGGTAAGATGACG
AAAAATAGAATTGAAGATACACTGTCTAGATTCGTTGAAAAAGAATTTGATATTTTAGTT
TCAACGACTATTATAGAAACAGGTGTAGATATACCAAACACCAATACACTTATTATCCAT
GATGCCGATCACTTAGGTCTTGCACAACTCTATCAATTACGTGGACGTGTAGGTAGATCA
GATAGAATTGCTTATGCGTATTTAATGTTTGATGGCTATAAAGATGTAAATGATGAAGCC
AAGAAACGTTTATCTGTTATTGAAGACTTTACCGATTTAGGTTCAGGGTTTAAAATTGCA
CTAAGAGATTTAGGTATTCGTGGTGCAGGTGATATTTTAGGTGAAGAACAATCAGGGTTT
ATTGATTCAGTAGGTATGGACCTGTATATGAAACTTCTTGATGAAGTTATGACGGGTAAA
GCATATGAATCTCCTAAGACAGATACGGTTGATCAAGTATTTTCAAAAAGACACATACCG
CCTACATATATTAATCATGACCCGGTCAGAATTGAAATACATAAAAGAATTGCTTCTTTA
AATAAGATGCAAGATTTAGAAGATTTAAAACAAGAATTACTAGATAGATTCGGTCCAATT
GATATAGATTTGATGACCTATATGTATGAGAAACTTTATAAGAAGTTAGGACATCAAGTC
GGTATTGAAAAAACTGTGCATGAAAAAGATTTCGTTAAAATGGTCATATCCTTAGAAAAA
AGTGAACTTATAGATGGTATGAGACTACTTTATGTAGCCTCCAAATCTAAGATGAATATC
AGACTTGGACAATCCAGAGGACATGTTGAAATCAACATGTCTACTAAACATGAAACAAAA
CACTGGCTATATTTAGCATGTGAATTATTAGAAATGTATATTGACTCAAAGGAGATAACA
ATTAATGAGTGA

>protein sequence
MTKNLLNSIYKDAAILNARTGDFKGVSHSYLQTMLSLRYDISNKNIFVVLPNLYEAQKYY
DTLSSIIDESKVLFYPMDQTLTSMMALGSPEFKNERLFTLRKLLQADDKYIIITTQEGIL
HRQLKPEDYERSVKKISVNQDYDLTDLTKKLIYDGYQFNYTVERPGEFSIRGSILDIYTH
DYKDPYRLDFFGDTLESIKTFDVQTQKSMNHITSIDIAPLNELFYTDELKEQALEKISKY
FSGFKLSEKEDKKLETDLMHIEERKRMDSLSMYIEFFNDEVTTILDFSNPYDLILVDSYK
MELNEETTYQDLLTYSHTMQGEAFIGLNFKVKLKELLKKNHLSFQIYSVSDKPSTDLGVL
DVDPFVGHLEQLILFINEYNGFNILISTKTMVSFERIKEHLTNHKISYSVNQFKDAMVVL
VEEDLPGSFIDLNHKFIILTEDTILDTKKKAKVRYRSVINQAVKIRDISELQNGDYVVHY
DFGIGQYIGLKTMTLSGDKRDYLHIVYDKDEALYVPTDQIDLVLKYKSYDQVKPKLSKLN
AKQWSKTKAQVKQKIKDLSDRLLNLYAKRHQAEGYQFSPRTDMLTSFEKDFNYDETIDQQ
KAIDAVFEDMESSKPMDRLIAGDVGYGKTEVALRAAFKAVVDAKQVAYLVPTTVLARQHY
LTFKDRFEKYGGSVALLSRYVSKREQKEVLEKIAKGYIDVVIGTHRLLSDDIVFKDLGLF
IIDEEQRFGVQHKEKIKEIKVNVDTLTLSATPIPRTLQMAMYGLKDLSMIDTPPLNRYPV
QTYVVERQPALIKEAIDRELSRGGQVFYLFNNTERMEAQVLKLQQLVPNARITYAHGKMT
KNRIEDTLSRFVEKEFDILVSTTIIETGVDIPNTNTLIIHDADHLGLAQLYQLRGRVGRS
DRIAYAYLMFDGYKDVNDEAKKRLSVIEDFTDLGSGFKIALRDLGIRGAGDILGEEQSGF
IDSVGMDLYMKLLDEVMTGKAYESPKTDTVDQVFSKRHIPPTYINHDPVRIEIHKRIASL
NKMQDLEDLKQELLDRFGPIDIDLMTYMYEKLYKKLGHQVGIEKTVHEKDFVKMVISLEK
SELIDGMRLLYVASKSKMNIRLGQSRGHVEINMSTKHETKHWLYLACELLEMYIDSKEIT
INEW