ACL_RS00150

Uniprot: A9NE92
Description: pyruvate formate-lyase 1-activating enzyme
EC number: 1.97.1.4
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical. {ECO:0000256|RuleBase:RU362053}.
Cofactor: COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000256|RuleBase:RU362053}; ; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000256|RuleBase:RU362053}
Enzyme regulation:
Function [CC]: FUNCTION: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine. {ECO:0000256|RuleBase:RU362053}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): pflA
Gene names (synonym):
Mass: 29,264
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; 4 iron, 4 sulfur cluster binding [GO:0051539]; [formate-C-acetyltransferase]-activating enzyme activity [GO:0043365]; lyase activity [GO:0016829]; metal ion binding [GO:0046872]
Gene ontology IDs: GO:0005737; GO:0016829; GO:0043365; GO:0046872; GO:0051539
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the organic radical-activating enzymes family. {ECO:0000256|RuleBase:RU362053}.
Protein families: Organic radical-activating enzymes family
Coiled coil:
Domain [FT]: DOMAIN 31 185 Radical_SAM. {ECO:0000259|Pfam:PF04055}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE92
MEROPS:
EnsemblBacteria KO: ABX80672
UniPathway: K04069
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR012838;IPR001989;IPR007197
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01087
ProDom: PF04055
SMART:
SUPFAM:
TIGRFAMs:

34620-35382(+)

>nucleotide sequence
ATGAAGGATTCTAATCTACTAGAAGTAGTAGGTAATGTACACTCAATAGAAACATTTGGT
GCCTTTGATGGACCAGGACTTAGATATGTATTATTCTTACAAGGTTGTCCTTTAAGATGT
AAATTCTGTCATAACAGAGACACTTGGGGCACAGAAGATAATAAGTTAATGACCGTAGAA
GAGATATTAAACGACTATAATAAATATAGAGCATTCTATAAAAAAGGTGGTTTAACAGTA
TCAGGTGGCGAAGCTACCTTACAAATCGGGTTTTTAACTGCATTATTCAAAGAAGCTAAA
AATAGAAATATACATACGTGTTTAGATACATCAGCAGGTACATTTAGTGAAGCAAGATTA
CCTGAGTTTGAAGAACTTCTCAAATACACAGATCTTGTGTTACTAGATATTAAACATATT
GATGATGAACGTCACAAATGGTTAACCGGTGCAAGCAATAAAAACATATTAAAGTTTGCA
AGACTGTTAAGTGATAAAAAAATACCTACGATATTAAGACATATACTCTTACCACAAATT
AACTCGCAAGATGTTTACTTAGAAAGACTAAGAACATTCATTGACTCTTTAGATAACTTT
ATTGGTATTGACATTCTTCCTTATCATACTAAAGGTATTATGAAGTGGGATAACATGGGT
ATTGAATATGAACTTAAAGATACACCAGAACCTACTAAAGAAGAGGTTTTAAGAGCAGAG
CATATACTTAAAGACGACTATAAGTATATGAAACTAAATTAG

>protein sequence
MKDSNLLEVVGNVHSIETFGAFDGPGLRYVLFLQGCPLRCKFCHNRDTWGTEDNKLMTVE
EILNDYNKYRAFYKKGGLTVSGGEATLQIGFLTALFKEAKNRNIHTCLDTSAGTFSEARL
PEFEELLKYTDLVLLDIKHIDDERHKWLTGASNKNILKFARLLSDKKIPTILRHILLPQI
NSQDVYLERLRTFIDSLDNFIGIDILPYHTKGIMKWDNMGIEYELKDTPEPTKEEVLRAE
HILKDDYKYMKLN