ACL_RS00165

Uniprot: A9NE95
Description: formate--tetrahydrofolate ligase
EC number: 6.3.4.3
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01543}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. {ECO:0000255|HAMAP-Rule:MF_01543}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 53 60 ATP. {ECO:0000255|HAMAP-Rule:MF_01543}.
Site:
Gene names (primary): fhs
Gene names (synonym):
Mass: 58,201
Subunit structure [CC]:
Gene ontology (GO): ATP binding [GO:0005524]; formate-tetrahydrofolate ligase activity [GO:0004329]; folic acid-containing compound biosynthetic process [GO:0009396]; tetrahydrofolate interconversion [GO:0035999]
Gene ontology IDs: GO:0004329; GO:0005524; GO:0009396; GO:0035999
Chain: CHAIN 1 527 Formate--tetrahydrofolate ligase. /FTId=PRO_0000333311.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family. {ECO:0000255|HAMAP-Rule:MF_01543}.
Protein families: Formate--tetrahydrofolate ligase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE95
MEROPS:
EnsemblBacteria KO: ABX80675
UniPathway: K01938
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_01543
PANTHER: IPR000559;IPR027417
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01268
SMART:
SUPFAM:
TIGRFAMs: SSF52540

37085-38669(+)

>nucleotide sequence
ATGAATACATATAATTACCTAATTGATGAATTACATATCTTAGATGATGAAATCATATCC
TATGGTAAGGATAAGTTTAAAATAGAGTTAAGTTTACAAGAAAGATTAAAGGATAAAGCA
CCTGGTAAACTTATTCTTGTAACATCGATTAACCCTACATCTAGTGGTGAAGGTAAAACA
ACACTTTCCATTGGTTTAGCCCAAGGGTTTAAGAAAAATGGTAAAGATGTTATGTTAGCA
CTTAGAGAACCATCCATGGGTCCTGTTTTTGGTATGAAGGGTGGTGCTACTGGTGGAGGT
GTTTCTATTTTAGAACCTTCATTAGATATCGACCTTCACTTTAATGGTGACATACATGCA
CTCACATCAGCGAATAATCTTTTATCAGCAATCATTGATAATCATATGTACTTTGGTAAT
GAATTAAATATTAAAGATGTTTACTGGCAACGCGCACTAGATGTTAATGATCGTAGCTTA
AGAGAAGTTAAGACAAAAGCTAGAGATGATAAATTTACCATTACAGCAGCAAGTGAAATG
ATGGCGATTTTGGCACTAGCAAGGGATTTTAAAGACTTAAAAGAAAGATTAAATAATATC
TTAATAGGTACCGATAAAGATGGAAAAGATTTATTTGTAAGTGACTTAAAGTGTGCGGAT
AGTTTAGCATTACTACTAAAGGATGCTATTAAGCCAAATTTAGTATTTGCTAAAGAAATG
GTACCAGCACTTGTTCATGCTGGACCATTTGCAAATATAGCACATGGATGTAATAGCGTT
ATAGCAACAAATACTGCTTTAAAGTTAGCTGATTATGTCATCACAGAAGCTGGTTTTGGT
GCAGATTTAGGTATGGAAAAGTTTTTACATATTAAGCAACCACATTTATACACAAAGGCT
AGTGTAGTGGTTGTAGTTGCAACTATTAAAGCGCTTAAACTACATGGTGGTGTTACAGAA
TCTAATTTAGATGAACCTAATATTGAGGCACTTTCTAAGGGATTAGAAAACATTGAAAAA
CACTTAGAAAATATTAAACTGTTTGGTTTAAACAGTGTTGTAGCACTTAATAAGTTTGAT
ACTGATTCAGAAGAGGAATTACAATTCTTAAAAAACTGGGCAAGAATCAATCACCTAAAT
TATGGTATTTCAGAAGGGTATAGTAAGGGTGGTGAAGGTACCAAAGACTTAGCTAAGTTA
GTTGAAAAAGTGGCATACGAACCATCTAAATTTAAGAGAATTTATTCTAATGAAGAAAAT
CATGAATATAAGATCAGAAAAATAGCGGAAAATATTTATGGGGCAAAGGATGTGATTTTC
TCTCAACAAGCAAAGAAGAAACTAAACCAATATAAACATTTAGAAATTCCTATTTGCATT
GCTAAAACACCACTTTCTTTATCTGGAGATCCTAAATTAAAGGGTAGACCAAGAGATTTT
GTTTTAGAGATTAGCGACATTAAAGTGTCTTTAGGTGCGAACCTTTTAGTAGTGCTTACA
AAAGGGATTAATACGATGCCTGGTTTAAATAATCGTCCACGGGCACTGGATTTTAAGTTG
GATGATAAAGGAGAACTTATATAA

>protein sequence
MNTYNYLIDELHILDDEIISYGKDKFKIELSLQERLKDKAPGKLILVTSINPTSSGEGKT
TLSIGLAQGFKKNGKDVMLALREPSMGPVFGMKGGATGGGVSILEPSLDIDLHFNGDIHA
LTSANNLLSAIIDNHMYFGNELNIKDVYWQRALDVNDRSLREVKTKARDDKFTITAASEM
MAILALARDFKDLKERLNNILIGTDKDGKDLFVSDLKCADSLALLLKDAIKPNLVFAKEM
VPALVHAGPFANIAHGCNSVIATNTALKLADYVITEAGFGADLGMEKFLHIKQPHLYTKA
SVVVVVATIKALKLHGGVTESNLDEPNIEALSKGLENIEKHLENIKLFGLNSVVALNKFD
TDSEEELQFLKNWARINHLNYGISEGYSKGGEGTKDLAKLVEKVAYEPSKFKRIYSNEEN
HEYKIRKIAENIYGAKDVIFSQQAKKKLNQYKHLEIPICIAKTPLSLSGDPKLKGRPRDF
VLEISDIKVSLGANLLVVLTKGINTMPGLNNRPRALDFKLDDKGELI