ACL_RS00170

Uniprot: A9NE96
Description: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase
EC number: 1.5.1.5; 3.5.4.9
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH. {ECO:0000255|HAMAP-Rule:MF_01576}.; CATALYTIC ACTIVITY: 5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01576}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. {ECO:0000255|HAMAP-Rule:MF_01576}.
Pathway: PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. {ECO:0000255|HAMAP-Rule:MF_01576}.
Active site:
Binding site: BINDING 184 184 NADP. {ECO:0000255|HAMAP-Rule:MF_01576}.; BINDING 225 225 NADP; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_01576}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 159 161 NADP. {ECO:0000255|HAMAP-Rule:MF_01576}.
Site:
Gene names (primary): folD
Gene names (synonym):
Mass: 30,601
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01576}.
Gene ontology (GO): methenyltetrahydrofolate cyclohydrolase activity [GO:0004477]; methylenetetrahydrofolate dehydrogenase (NADP+) activity [GO:0004488]; folic acid-containing compound biosynthetic process [GO:0009396]; histidine biosynthetic process [GO:0000105]; methionine biosynthetic process [GO:0009086]; purine nucleotide biosynthetic process [GO:0006164]; tetrahydrofolate interconversion [GO:0035999]
Gene ontology IDs: GO:0000105; GO:0004477; GO:0004488; GO:0006164; GO:0009086; GO:0009396; GO:0035999
Chain: CHAIN 1 277 Bifunctional protein FolD. /FTId=PRO_0000340573.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. {ECO:0000255|HAMAP-Rule:MF_01576}.
Protein families: Tetrahydrofolate dehydrogenase/cyclohydrolase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NE96
MEROPS:
EnsemblBacteria KO: ABX80676
UniPathway: K01491
CDD:
Gene3D:
HAMAP: 3.40.50.720
InterPro: MF_01576
PANTHER: IPR016040;IPR000672;IPR020630;IPR020867;IPR020631
PIRSF:
PRINTS:
PROSITE: PR00085
Pfam: PS00767
ProDom: PF00763;PF02882
SMART:
SUPFAM:
TIGRFAMs: SSF51735

38668-39502(+)

>nucleotide sequence
ATGTTATTAGATGGAAGAAAAACAGCAGATATACTTAATGAAGCACTTCGTGTTAAAGTT
CAATATTTAACTAAAAAGCCTAAATTAGAAATCATTTTAATTGGTAATGACGAGGCAAGC
AAAAGTTATGTTAAAGGAAAGCTTAATACGGCCTCTAATTTAGGTATGGAAGTTCATATA
AATTATCTAGATGAAGCAATTGATCAATTAAGTGTAGAAGCTTTAATACAAAAGTTAAAC
ACAGATAAAGGTGTTCATGGTATATTACTTCAATTACCTATTCCTAAACACTTGGATTCA
GATTATCTTATATCTTTAATAGATTATAAAAAAGATGTTGATGGATTTCATACAATGAAT
CAAGGTTTACTATTTCAAAAAAAGGACGGTATTAGACCTGCTACACCGCTAGGTATAATG
ATGTTACTAGATTTTTATAACATTCCAATTGAAGGTAAACATGTGGTTATTATTGGTAGA
AGTCAAATCGTTGGTGCACCACTATCCAAAATGTTTTTAGATCGTAATGCGACAGTAACG
ATTACACACTCTAAAACTAAAAATCTTCCATCCATCACCAAACAAGCAGATATACTGGTA
GCTGCAATTGGTAAACCTAAATTTGTTACTAAAGATATGGTCAAAGTAGGTGCTGTTGTC
GTTGATGTTGGGATTAACCGAGTAGATAAAAAATTAGTAGGAGACGTCGACTTTGAACAT
GTAGAGCCGATTGCTTCATATATTACACCTGTACCTAAAGGTGTTGGTCCCATGACAATT
TGTGCACTGGCACATAATTTATATGCCCTGTATCTTAAACAGGAGAAAGAATAG

>protein sequence
MLLDGRKTADILNEALRVKVQYLTKKPKLEIILIGNDEASKSYVKGKLNTASNLGMEVHI
NYLDEAIDQLSVEALIQKLNTDKGVHGILLQLPIPKHLDSDYLISLIDYKKDVDGFHTMN
QGLLFQKKDGIRPATPLGIMMLLDFYNIPIEGKHVVIIGRSQIVGAPLSKMFLDRNATVT
ITHSKTKNLPSITKQADILVAAIGKPKFVTKDMVKVGAVVVDVGINRVDKKLVGDVDFEH
VEPIASYITPVPKGVGPMTICALAHNLYALYLKQEKE