ACL_RS00225

Uniprot: A9NEA5
Description: inorganic pyrophosphatase
EC number: 3.6.1.1
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Diphosphate + H(2)O = 2 phosphate. {ECO:0000256|HAMAP-Rule:MF_00209}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00209}; ; Note=Binds 4 Mg(2+) ions per subunit. Other metal ions can support activity, but at a lower rate. Two Mg(2+) ions are required for the activation of the enzyme and are present before substrate binds, two additional Mg(2+) ions form complexes with substrate and product. {ECO:0000256|HAMAP-Rule:MF_00209}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 66 66 Magnesium 1. {ECO:0000256|HAMAP-Rule:MF_00209}.; METAL 71 71 Magnesium 1. {ECO:0000256|HAMAP-Rule:MF_00209}.; METAL 71 71 Magnesium 2. {ECO:0000256|HAMAP-Rule:MF_00209}.; METAL 103 103 Magnesium 1. {ECO:0000256|HAMAP-Rule:MF_00209}.
Nucleotide binding:
Site:
Gene names (primary): ppa
Gene names (synonym):
Mass: 20,724
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; inorganic diphosphatase activity [GO:0004427]; magnesium ion binding [GO:0000287]; phosphate-containing compound metabolic process [GO:0006796]
Gene ontology IDs: GO:0000287; GO:0004427; GO:0005737; GO:0006796
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the PPase family. {ECO:0000256|HAMAP-Rule:MF_00209}.
Protein families: PPase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEA5
MEROPS:
EnsemblBacteria KO: ABX80685
UniPathway: K01507
CDD:
Gene3D: cd00412
HAMAP: 3.90.80.10
InterPro: MF_00209
PANTHER: IPR008162
PIRSF: PTHR10286
PRINTS:
PROSITE:
Pfam: PS00387
ProDom: PF00719
SMART:
SUPFAM:
TIGRFAMs: SSF50324

54834-55377(+)

>nucleotide sequence
ATGAATATATGGCATGACATAAATCCATCCCGCATAACACCGGAGCGTTTTATTGTTTGT
ATTGAGATTACTAAAGGTAGTAAAAAGAAGTACGAACTAGATAAAGAAACAGGGATGATT
ATTTTAGATAGAGTATTATACACATCAGCACATTATCCAGCGAACTACGGATTTATTCCT
AGAACATATGCTGGTGACAATGACCCACTTGATGTATTAGTACTATGTCAAGAAGACATT
GAGCCAATGTCTTTAGTAGAAGTGTATCCAATTGGTGTCATTAAAATGATTGATAGTGAT
GAAGTAGATGAGAAAATTATTGCAATTCCTTATGGGGATCCATCTATGACACAGTATAAA
GATTTAAAGGGGTTACCACATCATTTGCTTTCTGAGATTTCTCACTTTTTTGAAGTGTAT
AAATCCCTAGAAGGAAAGAAAACATACATTCTTGACATTGAGTCAAGAGATGAGGCAATT
AAAGTTGTATCTGAGGCTATGGTCTCATACGACAAAGTATTTACAAATAAGAAAGAGAAG
TAA

>protein sequence
MNIWHDINPSRITPERFIVCIEITKGSKKKYELDKETGMIILDRVLYTSAHYPANYGFIP
RTYAGDNDPLDVLVLCQEDIEPMSLVEVYPIGVIKMIDSDEVDEKIIAIPYGDPSMTQYK
DLKGLPHHLLSEISHFFEVYKSLEGKKTYILDIESRDEAIKVVSEAMVSYDKVFTNKKEK