ACL_RS00275

Uniprot: A9NEB6
Description: thiamine biosynthesis lipoprotein
EC number: 2.7.1.180
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP. {ECO:0000256|RuleBase:RU363002}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|PIRSR:PIRSR006268-2}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|PIRSR:PIRSR006268-2}; ; Note=Magnesium. Can also use manganese. {ECO:0000256|PIRSR:PIRSR006268-2}
Enzyme regulation:
Function [CC]: FUNCTION: Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein. {ECO:0000256|RuleBase:RU363002}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 189 189 Magnesium; via carbonyl oxygen. {ECO:0000256|PIRSR:PIRSR006268-2}.; METAL 314 314 Magnesium. {ECO:0000256|PIRSR:PIRSR006268-2}.; METAL 318 318 Magnesium. {ECO:0000256|PIRSR:PIRSR006268-2}.
Nucleotide binding:
Site:
Gene names (primary): apbE
Gene names (synonym):
Mass: 40,587
Subunit structure [CC]:
Gene ontology (GO): metal ion binding [GO:0046872]; transferase activity [GO:0016740]; protein flavinylation [GO:0017013]
Gene ontology IDs: GO:0016740; GO:0017013; GO:0046872
Chain: CHAIN 25 360 FAD:protein FMN transferase. {ECO:0000256|RuleBase:RU363002}. /FTId=PRO_5005967830.
Signal peptide: SIGNAL 1 24 {ECO:0000256|RuleBase:RU363002}.
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the ApbE family. {ECO:0000256|RuleBase:RU363002}.
Protein families: ApbE family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEB6
MEROPS:
EnsemblBacteria KO: ABX80696
UniPathway: K03734
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR024932;IPR003374
PIRSF:
PRINTS: PIRSF006268
PROSITE:
Pfam:
ProDom: PF02424
SMART:
SUPFAM:
TIGRFAMs: SSF143631

63790-64873(+)

>nucleotide sequence
ATGAAAAAAATATGGGCAGTTATCAACTTAATATTAATCAGTGTTCTTTTTTCTGCTTGT
ACACAACAAGCAATTGATAACAGTTTAGCCATATCATCTATGGATACATTTATTGGTGGC
ACAGTACATGCACCAAAAGCTGTTTGGGATAAAGCGGATAAAAAACTAAAAGAAATGTAT
ATGCTCTACCACGAGTTAACCGATAACTATAGAGGTTATGAGGGTGTACAAGGTGTTTAC
TATATTAATAATTTAGTAGAAACAACGAAAACTGACCAAACAGTTGAGATTAAAAAAGAA
CTTTATGATCTATTAAGTTTAGGTGTAGAACTCTATGAATTTACAGATGGATACTTTGAT
ATGTCCATTGGTAAGATCATCGATGTTTGGAAAAACTTAATTGATGAAAGTGATTATACG
GGTCAAGAAATACCTAAAGAAGATATCGATTATGCGATAAACTTAGTGGATGCTATTGAT
ATCATTGAAGATCCGGTGATTTTAAGTGAAGTAGATAATAAATATTACGTGACACTTAAA
TATGGTGCAAAACTAGACTTAGGTGCGCTTGCTAAAGGGTATGTCACTCAGTTAGCAGCA
GATTATTTAACGGATTTAGGTTTAAGTACCTATCTCATTAGTGGTGGTGGCTCATCCATG
TTATATGGTGAAGGTAATCCAAAAACTGAAGATGGTAGTTACAGAACAGGACTTATTAAT
CCACAAGATGTTATTGATAACTTAGATATTATTGGATATCGACCTAAAAATTACGGTATA
TATACAGCTAAAAATTATAACTTCACAACATCAGGAAGTTATAATCAATTTATCTTAAGT
GAAGGTGTGATGTATCATCACATTGTCTCACCTAAAACAAAACGTCCGGTAAATCATTAT
CTAACACTTTCTATTGTTGGAACAGATGCAGGATTTAATGATGGTTTATCCACAGCATTA
TTTAGTATGCCACCTGAAGTGTTAGAACAGTTTTTAGAAACGAATGGTTATGAGGTCTAT
ACATATTTATTTAATAATACTTATAAATCCTATAATCAATCTGAAAACTTTAGACCACTT
TAA

>protein sequence
MKKIWAVINLILISVLFSACTQQAIDNSLAISSMDTFIGGTVHAPKAVWDKADKKLKEMY
MLYHELTDNYRGYEGVQGVYYINNLVETTKTDQTVEIKKELYDLLSLGVELYEFTDGYFD
MSIGKIIDVWKNLIDESDYTGQEIPKEDIDYAINLVDAIDIIEDPVILSEVDNKYYVTLK
YGAKLDLGALAKGYVTQLAADYLTDLGLSTYLISGGGSSMLYGEGNPKTEDGSYRTGLIN
PQDVIDNLDIIGYRPKNYGIYTAKNYNFTTSGSYNQFILSEGVMYHHIVSPKTKRPVNHY
LTLSIVGTDAGFNDGLSTALFSMPPEVLEQFLETNGYEVYTYLFNNTYKSYNQSENFRPL