ACL_RS00300

Uniprot: A9NEC1
Description: adenosine deaminase
EC number: 3.5.4.33
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Adenine(34) in tRNA + H(2)O = hypoxanthine(34) in tRNA + NH(3). {ECO:0000256|HAMAP-Rule:MF_00972}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00972}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00972}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2). {ECO:0000256|HAMAP-Rule:MF_00972}.
Pathway:
Active site: ACT_SITE 57 57 Proton donor. {ECO:0000256|HAMAP-Rule:MF_00972}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 55 55 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00972}.; METAL 55 55 Zinc; via pros nitrogen; catalytic. {ECO:0000256|HAMAP-Rule:MF_00972}.; METAL 85 85 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00972}.; METAL 88 88 Zinc; catalytic. {ECO:0000256|HAMAP-Rule:MF_00972}.
Nucleotide binding:
Site:
Gene names (primary): tadA
Gene names (synonym):
Mass: 16,350
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00972}.
Gene ontology (GO): tRNA-specific adenosine-34 deaminase activity [GO:0052717]; zinc ion binding [GO:0008270]; tRNA wobble adenosine to inosine editing [GO:0002100]
Gene ontology IDs: GO:0002100; GO:0008270; GO:0052717
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the cytidine and deoxycytidylate deaminase family. {ECO:0000256|HAMAP-Rule:MF_00972}.; SIMILARITY: Contains 1 CMP/dCMP-type deaminase domain. {ECO:0000256|HAMAP-Rule:MF_00972}.
Protein families: Cytidine and deoxycytidylate deaminase family
Coiled coil:
Domain [FT]: DOMAIN 4 113 CMP/dCMP-type deaminase. {ECO:0000259|PROSITE:PS51747}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEC1
MEROPS:
EnsemblBacteria KO: ABX80701
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00972
PANTHER: IPR016192;IPR002125;IPR016193;IPR028883
PIRSF:
PRINTS:
PROSITE:
Pfam: PS00903;PS51747
ProDom: PF14437
SMART:
SUPFAM:
TIGRFAMs: SSF53927

66913-67370(+)

>nucleotide sequence
ATGATAAAAAATAGTCGTGAGTACTTCATGAATGAGGCTCTAAAAGAAGCTAAAAAAGCT
AATGATAAAGACGAGGTTCCTGTTGGTGCAGTTGTCGTATTAGACGGTAAAATTATTGCA
AGAGCACATAATTTGCGTGAAAGTAGACAATCCATCCATGCTCATGCTGAATTTTTAGCA
ATAGAAAAAGCAGCAAAAAAAATTGGTAGTTGGCGTTTAGAAAATGCAGATGTATACGTT
ACATTAGAGCCTTGCCCAATGTGTGCAGGTGCAATGATACAAGCGCGTATTAAGAATTTG
TATTACGGCGCGAAAGACCCAAAGACTGGTGCAGTTGAAAGTGTTATAAAACTGCTTGAC
AATCCCTTTAATCATAAGATATACTATGAAGGTGGATTGTTGATGGATAAGTGTTCAAAC
ATCCCTTTCGACATTCTTTAAAAATAAGCGAAAATAA

>protein sequence
MIKNSREYFMNEALKEAKKANDKDEVPVGAVVVLDGKIIARAHNLRESRQSIHAHAEFLA
IEKAAKKIGSWRLENADVYVTLEPCPMCAGAMIQARIKNLYYGAKDPKTGAVESVIKLLD
NPFNHKIYYEGGLLMDKCSNIPFDIL