ACL_RS00545

Uniprot: A9NEF6
Description: translation initiation factor IF-1
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex. {ECO:0000255|HAMAP-Rule:MF_00075}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): infA
Gene names (synonym):
Mass: 8,222
Subunit structure [CC]: SUBUNIT: Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly. {ECO:0000255|HAMAP-Rule:MF_00075}.
Gene ontology (GO): cytoplasm [GO:0005737]; ribosome binding [GO:0043022]; rRNA binding [GO:0019843]; translation initiation factor activity [GO:0003743]
Gene ontology IDs: GO:0003743; GO:0005737; GO:0019843; GO:0043022
Chain: CHAIN 1 72 Translation initiation factor IF-1. /FTId=PRO_0000338742.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IF-1 family. {ECO:0000255|HAMAP-Rule:MF_00075}.; SIMILARITY: Contains 1 S1-like domain. {ECO:0000255|HAMAP-Rule:MF_00075}.
Protein families: IF-1 family
Coiled coil:
Domain [FT]: DOMAIN 1 72 S1-like. {ECO:0000255|HAMAP-Rule:MF_00075}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEF6
MEROPS:
EnsemblBacteria KO: ABX80736
UniPathway: K02518
CDD:
Gene3D:
HAMAP: 2.40.50.140
InterPro: MF_00075
PANTHER: IPR012340;IPR006196;IPR022967;IPR004368
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50832
ProDom: PF01176
SMART:
SUPFAM: SM00316
TIGRFAMs: SSF50249

95925-96144(+)

>nucleotide sequence
ATGGCAAGAGAAGATTTAATAGAAGTTGAAGCAAAGGTTAAAGAAATTTTACCGAACACA
AAGTTTATAGTTGAGCTAGACAACGGTCACACCGTTGTAGCACACGTATCTGGTAAAATC
CGTATCCACAACATACGCATTTTACCTGGTGATAAAGTAACAGTTGAATTATCACCATAT
GATTTATCACGCGGACGTATAACATACCGCAAAAAATAA

>protein sequence
MAREDLIEVEAKVKEILPNTKFIVELDNGHTVVAHVSGKIRIHNIRILPGDKVTVELSPY
DLSRGRITYRKK