ACL_RS00565

Uniprot: A9NEG0
Description: DNA-directed RNA polymerase subunit alpha
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000256|HAMAP-Rule:MF_00059}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000256|HAMAP-Rule:MF_00059}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoA
Gene names (synonym):
Mass: 37,022
Subunit structure [CC]: SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000256|HAMAP-Rule:MF_00059}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351
Chain:
Signal peptide:
Domain [CC]: DOMAIN: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements. {ECO:0000256|HAMAP-Rule:MF_00059}.
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase alpha chain family. {ECO:0000256|HAMAP-Rule:MF_00059}.
Protein families: RNA polymerase alpha chain family
Coiled coil:
Domain [FT]: DOMAIN 21 228 RPOLD. {ECO:0000259|SMART:SM00662}.
Motif:
Region: REGION 1 229 Alpha N-terminal domain (alpha-NTD). {ECO:0000256|HAMAP-Rule:MF_00059}.; REGION 247 330 Alpha C-terminal domain (alpha-CTD). {ECO:0000256|HAMAP-Rule:MF_00059}.
EMBL: CP000896
ProteinModelPortal: A9NEG0
MEROPS:
EnsemblBacteria KO: ABX80740
UniPathway: K03040
CDD:
Gene3D:
HAMAP: 2.170.120.12
InterPro: MF_00059
PANTHER: IPR011262;IPR011263;IPR011773;IPR009025;IPR011260
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01000;PF03118;PF01193
SMART: PD001179
SUPFAM: SM00662
TIGRFAMs: SSF55257;SSF56553

97078-98071(+)

>nucleotide sequence
GTGAAAGATTTAAAATTTGAAAAACCAACATCTGTCGAAGAAATATCTCAAGAAGGTAAC
TTGGGACGTTTCTTAATCAAACCACTTGAACGCGGTTATGGGTTAACCTTAGGTAATGCA
TTAAGACGTGTCTTATTATCATCGTTACCAGGTGCTGCAATCGTCAATGTTAAAATTGAA
GGTGTGGAGCACGAGTTTTCTACCATTGAAGGCGTTTATGAAGATGTGATGGGAATCGTT
CTTAACCTTAAAAAGGTCGTATTCTCAGTTGATTCACAAGATCCACAGTATGAACAACAA
TTAGAATTATATGCAGAAGGTCCAGCTGTGATTACTGCAGGTGATTTTAATTTGGCAGAC
GACATTGAAGTTGTCAATAAAGACCAACATATTGCAACTCTAGCAGAAGGTGCTAAGTTC
AATATGACAGTTACAGTTAGACGTGGTATTGGTTATGTACCAGCTGACCAAAATAAACAA
TATTCTAAATACGAATTAAACGTTATTCCAATTGACTCACTTTATACACCAGTTGAACGT
GTAATCTACAGTGTAGAAAAAACACGTGGTGATTTAGATGAATTAACAATTGAAATCGAA
ACAAACGGTGCTATCTTAGCAAAAGAAGCATTAGCCCTCGCATCTAAGATGTTAGTGGAT
CACTTCCAAGTACTTGTAGATATTTCTGAAAAAGCTAGTGAATTTGATTTCATTCGCGAT
ATTGAAGAAGAACCAGCAAGCAAAAAATCAGATACTAAGATTGAACAACTTGATTTATCA
GTTCGTTTATTCAACTCACTAAAACGTAGTGGTATCACTACAGTTGGTGAATTACTAAAA
TTATCTGAAGAAGAAGTTATGAGACTACGTTCATTAGGTAGAAAATCATTCAAAGAATTA
AAAGAAAAATTAGCAGAACATGGACTTGAATTCGAGCACTCTACTTCAAAAGAAGCAAGA
TTCGGATTTGATTCAGACGAAGATAAGGAGTAA

>protein sequence
VKDLKFEKPTSVEEISQEGNLGRFLIKPLERGYGLTLGNALRRVLLSSLPGAAIVNVKIE
GVEHEFSTIEGVYEDVMGIVLNLKKVVFSVDSQDPQYEQQLELYAEGPAVITAGDFNLAD
DIEVVNKDQHIATLAEGAKFNMTVTVRRGIGYVPADQNKQYSKYELNVIPIDSLYTPVER
VIYSVEKTRGDLDELTIEIETNGAILAKEALALASKMLVDHFQVLVDISEKASEFDFIRD
IEEEPASKKSDTKIEQLDLSVRLFNSLKRSGITTVGELLKLSEEEVMRLRSLGRKSFKEL
KEKLAEHGLEFEHSTSKEARFGFDSDEDKE