ACL_RS00710

Uniprot: A9NEI9
Description: cysteine--tRNA ligase
EC number: 6.1.1.16
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). {ECO:0000255|HAMAP-Rule:MF_00041}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|HAMAP-Rule:MF_00041}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00041}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site: BINDING 267 267 ATP. {ECO:0000255|HAMAP-Rule:MF_00041}.
Calcium binding:
DNA binding:
Metal binding: METAL 28 28 Zinc. {ECO:0000255|HAMAP-Rule:MF_00041}.; METAL 207 207 Zinc. {ECO:0000255|HAMAP-Rule:MF_00041}.; METAL 232 232 Zinc. {ECO:0000255|HAMAP-Rule:MF_00041}.; METAL 236 236 Zinc. {ECO:0000255|HAMAP-Rule:MF_00041}.
Nucleotide binding:
Site:
Gene names (primary): cysS
Gene names (synonym):
Mass: 50,682
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00041}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; cysteine-tRNA ligase activity [GO:0004817]; zinc ion binding [GO:0008270]; cysteinyl-tRNA aminoacylation [GO:0006423]
Gene ontology IDs: GO:0004817; GO:0005524; GO:0005737; GO:0006423; GO:0008270
Chain: CHAIN 1 434 Cysteine--tRNA ligase. /FTId=PRO_0000332776.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000255|HAMAP-Rule:MF_00041}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]:
Motif: MOTIF 30 40 "HIGH" region.; MOTIF 264 268 "KMSKS" region.
Region:
EMBL: CP000896
ProteinModelPortal: A9NEI9
MEROPS:
EnsemblBacteria KO: ABX80769
UniPathway: K01883
CDD:
Gene3D: cd00672
HAMAP: 3.40.50.620
InterPro: MF_00041
PANTHER: IPR015803;IPR024909;IPR014729;IPR032678;IPR009080
PIRSF: PTHR10890
PRINTS:
PROSITE: PR00983
Pfam:
ProDom: PF01406
SMART:
SUPFAM:
TIGRFAMs: SSF47323

127749-129054(+)

>nucleotide sequence
ATGTTAAAAATATATAACTCACTGTCAAACAAAATTGAAGAATTTAAGCCAATTCATGAA
AAAAAAGTAAATATGTATGTGTGTGGACCTACAGTCTATGATGATATACATATTGGTAAT
GGTAGACCCGTTGTCTTTTTTGATGTTGTTAAGCGTTACTTACAATATCTAGATTATGGC
GTTAAATACGCATCTAACATCACTGATGTGGATGATAAGATCATTGACAGAGCCCAAAAA
CTACATATAACTGAAAAAGAACTAGCTACTACTTATACCAACAATTTTTTTGAAATAGCT
AAAAAAATTGGTGGATATAATTTTGATGTCACACCACATGCAACCAACTATATTGAAGAA
ATGATTAAGTTTATTGGTGAACTAATTTCTGATGGGTTTGCATATAAGACCCAAAGTGGT
GTCTATTTTAGAGTGGATAAAATCAAAGACTATGGGATATTGTCGAACCAACAGGTTAAA
GACTTAAAAACTGGAGTCCGTATTGATTTAGAAACTGATAAAGAAAAAGACTATGATTTT
GCTTTATGGAAGAACACAGAAGAAGGTATTAAATATCATGCACCTTGGGGTGATGGTCGT
CCTGGGTGGCATACAGAGTGTGTTGTCATGACAAACGAAATCTTTGGTGGTGAAATTGAT
ATTCACGGTGGTGGGTTTGATTTAAAATTTCCACACCATGAAAATGAAATTGCACAGTCT
GTTGCAAAACATGATCACCATCTAGCGAAGTACTGGATGCATGTTGGTAGACTGGATTTA
GCTAATGAAAAGATGAGTAAATCATTAGGTAATGATATCAAACTTAAAGACCTTGTTCAA
GTCTATAATCCAAATGCATATCGCTTAATGTTGTTAGCACATCATTACCGAGCGCCAATT
CAGTTTAGTGATGACTTAATTGAACAATATCAAAAGGCTTATGATAAAATATCATATACA
CTGAATAAGTGGCATTTTCATATGATATTAAACAATATCAATGAAAATGGATTAGATCAT
GAAAGTATGGAATACTTTGAAGGATTTATGAACGATGATTTTGCTACACCTAGAGTTATT
TCCTTAATTGATAAACTCATTAAAGACTTAAATAAAGCATTTAAAGTAGAAAACTACAAC
ACGATCATACAAATACTATCCACATTAGGTATAAACCCTAATATCCTTGAAGTTTTAGAA
GATGATATTCAAAACTATAATAACTGGCAACAAGCAAGGTTAGAGAAAAATTATGAGAAG
GCTGATATCTATAGAAAACCATTATTAGATAAAGGTTTTATCTAA

>protein sequence
MLKIYNSLSNKIEEFKPIHEKKVNMYVCGPTVYDDIHIGNGRPVVFFDVVKRYLQYLDYG
VKYASNITDVDDKIIDRAQKLHITEKELATTYTNNFFEIAKKIGGYNFDVTPHATNYIEE
MIKFIGELISDGFAYKTQSGVYFRVDKIKDYGILSNQQVKDLKTGVRIDLETDKEKDYDF
ALWKNTEEGIKYHAPWGDGRPGWHTECVVMTNEIFGGEIDIHGGGFDLKFPHHENEIAQS
VAKHDHHLAKYWMHVGRLDLANEKMSKSLGNDIKLKDLVQVYNPNAYRLMLLAHHYRAPI
QFSDDLIEQYQKAYDKISYTLNKWHFHMILNNINENGLDHESMEYFEGFMNDDFATPRVI
SLIDKLIKDLNKAFKVENYNTIIQILSTLGINPNILEVLEDDIQNYNNWQQARLEKNYEK
ADIYRKPLLDKGFI