ACL_RS00840

Uniprot: A9NEL7
Description: DNA-directed RNA polymerase subunit beta
EC number: 2.7.7.6
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). {ECO:0000255|HAMAP-Rule:MF_01321}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000255|HAMAP-Rule:MF_01321}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoB
Gene names (synonym):
Mass: 141,439
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01321}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; ribonucleoside binding [GO:0032549]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351; GO:0032549
Chain: CHAIN 1 1271 DNA-directed RNA polymerase subunit beta. /FTId=PRO_1000086360.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta chain family. {ECO:0000255|HAMAP-Rule:MF_01321}.
Protein families: RNA polymerase beta chain family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: ABX80797
UniPathway: K03043
CDD:
Gene3D: cd00653
HAMAP: 2.30.150.10;2.40.270.10;2.40.50.150;3.90.1110.10
InterPro: MF_01321
PANTHER: IPR019462;IPR015712;IPR007120;IPR010243;IPR007121;IPR007644;IPR007642;IPR007645;IPR007641;IPR014724
PIRSF: PTHR20856
PRINTS:
PROSITE:
Pfam: PS01166
ProDom: PF04563;PF04561;PF04565;PF10385;PF00562;PF04560
SMART:
SUPFAM:
TIGRFAMs:

154539-158355(+)

>nucleotide sequence
ATGAGTTATAAAACCGTAAAATACGGAAAAAAAGCAGAACGACGTGATTATTCAAAAATG
TTACATCAGGTTGAACTGCCGAATCTTATCGAGATACAGACTAAATCCTTTGAGGAGTTT
GTAGAAACTGGTATTGATGAACTGTTAAAAGACATTTCACCAATTGAAGGACACAATGGT
GACTTGAAACTCTACTTTGAGGAGAGTTCATTATCCGAACCAAAGTATTCTACTATTGAA
ACCAAGATTCGTGATCTAAACTATTCTAGACAATTATCTGCTAGAGTTAAATTAGAAAAC
GCAATTACAGGTGAAGTAAGAGAATCCACCGTATTAATGACAGACCTTCCTATGATGACA
CCATCAGGGACTTTTATCATTAATGGCGCTGAACGTGTAGTTGTATCCCAAATTGTACGT
TCATCAGGTGTGTATTATACATCTGAATTGGATAAAAAAATAAACCAAATTAAGTACTCT
GCACAAGTGATCCCAACACGTGGTGCTTGGATAGAGTTTGAACAAGGTTCTAAAGAGATT
TTATATGCTAAACTTGACCGTTCTAAGAAAGTTCCATTAACAACTTTCATTAGAGCATTA
GGGTTTACTACTAAAAAAGATATTGAAGAAACTTTTGGTCGCTCAAGCTTAATTACCAAC
ACTTTTGAAAAAGATGAAACCAAATCACCAAATGAAGCGGTTATTGATCTTTACTCAAAA
TTACGTCAAGGTGAAAAAGTTCCGGCTGATGCAGCACGCGAATTCATTAGAATGCGCTTA
TTTGATGCTAGACGTTATGATTTAGCTAAAGTAGGACGTTATAAATTCATTAAGAAATTA
GACGTATTAGCAAGAGCAGAAGGTACATACTTAGCAAACGATATCATCTTAGATGGTGAA
GTGTTAGTTGCTAAAGATACACACCTTACAAAAGAAGTTATTCAAATTTTAGCTCAAAAT
CGTGATGCATTTAGAAAACAATTAATTACTAAAGAAAATAACTTACAAAATGAAACTGCT
GATGAAATATTAGCAACTACTTTACCAGAAGGTGGTAACACACTTTATGCTAAAGAAAAT
GTTGTGAACTTAAAAACTGGTGAAGTTTTAGTTAAGAAAAATGAAGCTATTACTGAAGAA
GTTTTAACAAACTTAAGAAAAAATAGACATTCAATTGACGAAAAAGTCATCAAATACTTC
TTAACAGAAGACGTTTACAAAAAAGAATCATTACGCCAAGGCGTTATTTCTGAAATTCTT
GATGTATATGTATATGATGATGCAGGCGATAAATCAAACGTTATCCGCATTATCGGTAGT
GACCAAAGAGAAGATAGAATCTTCGTAGCAGTATCTGATATTGTTGCAAGTATTTCATAC
TTCCTAAACCTATATGATGGTTTAGGTAATGTAGATGATATCGACCACTTAGGTAATAGA
CGTCTAAGATTAATTGGAGAGCTATTAAAAAACCAATTTAGAATTGGTTTAGCAAGAGCT
GAAAAGAACATCAAAGATAAGATGTCAACAACAAACTTTAATGATGCAACACCTGCAAAC
GTTATTAACATGACGCCACTTGTAGGTGCTATTAAAACATTCTTTGGTTCGTCACAATTA
TCACAATTCATGGACCAAATTAACCCACTAGCAGAGTTAACTCAAAAACGTAGAGTATCT
GCCTTAGGTACCGGTGGTATTGCAAGAGATAGAGCTGGGGTTGAAGTACGTGACGTGCAT
AACTCTCACTACGGTAGACTCTGTCCAATTGAAACACCAGAAGGTCCTTCAATTGGTTTA
ATCTCCTCACTTGCTACATATGCTAAAGTCGATGAATATGGCTTTATTAAGACACCATTC
TTAAAAGTTGTACAAAACGGAAATGAAACATCAGTGACTAAAGAAGTTATTTATTTAACA
GCTGACGAAGAATCAGATCACGTGATTGCATCTGCTGCTACACCAATGGATGAACATGGT
TTATTCATTGAAAGTAGAGTGATTGCAAGACGTAATGGTGAAACTGGTATTTATGATGCT
AATGAAATCACTGCAATGGACGTTAGTCCAAAACAGGTCGTATCTGTTGCGACATCTTCC
ATTCCATTCCTAGAACACGATGATGCTTCTCGTGCGCTTATGGGTGCGAACATGCAACGT
CAAGCAGTACCTTTATTACAACCTACATCACCGATTGTTGGTACAGGGGTTGAATACCGT
GCAGCTAAAGACTCTGGTGCTGTAGTCATTGCTAAAAACTCAGGTTATGTAACCTATGTC
GATGGTAAGAAGATTATCATCACACCAGAACCAACTGATACAATTAAATCAGGATCTAAA
ACACTATATAAAGTGGGCGGAGAATTTGATTATGGTATCGCTAAGAAACTATATGAAACC
AAATCAGTTCAAAATGATCAATATGATTTAGTTCAATTCTTTAGATCTAACCAAGATACT
TTAATATTACAAAAACCAATCGTTATGCAAGGTGAGTACGTTGATGCAGGCGATGTCATT
GCTGATGGCCCTTCAACTGAAAATGGTGAATTAGCATTAGGACGTAACGTAACCGTGGCA
TTCATGACATGGGAAGGTTATAACTATGAAGATGCTGTCATCATGAGTGAAGACTTAGTG
AAGCATGACGTGTATACATCCATTCACATTGATGAATATGAAATTGAAACAAGAGATTTA
AAACAAGCTAATGGTAAAGAAGAAATCACAAGAGAAATTCCTAACGTTGGTCAAGAAGCA
ATTAAATTCTTAGATGAACGCGGTATTATCGTACCAGGCTCTGAAGTTAAAGAAGGCGAC
ATCTTAGTTGGTAAAATCACACCAAAAGGTGTATTTGAACCAACTCCATCAGAAAAATTA
ATTCACGCTGTTATTGGTGATAAAGCTAAAGAGTATAGAGATTCATCCCTACGTGTACCT
CATGGTGGCGGTGGTGTAGTTCAATCTGTTCAATACTTCAGTAAGAAAAATGGTGATCAA
TTACCAACTGGTGTAAACGAACAAATTAGAGTTTATATTGCTAAAAAGCGTAAAATCACT
GAAGGTGATAAGATGGCTGGGCGTCACGGTAACAAGGGTGTTATTTCTAAAATTTTACCA
AGAGAAGATATGCCATATTTAGCAGATGGAACATATGTTGACATCATGTTAAACCCATTA
GGGGTTCCATCACGTATGAACATTGGACAAATCTTAGAAATTCACTTAGGAATTTCGGCT
CAAAAATTAGGTATTAAAGTTGCATCCCCAGTATTCGATGGTGTGACTAACGATGAGTTA
GGCGCAATCATGGAAGAAGCAGGTATTGCACCAGATGGTAAGACTACATTATATGATGGT
CGTACCGGAGAACCTTATAACAACCGTATTTCGGTTGGTGTAATGTACATGATCAAACTA
TCGCATATGGTGGATGATAAACTACATGCTAGAAACGTTGGACCTTATACATTAGTTACA
CAACAACCAATGGGTGGTAAAGCTCAAAACGGTGGACAACGTTTCGGGGAGATGGAAGTT
TGGGCACTTTATGCTTATGGTGCTGCACATACACTTCAAGAAATGTTAACGATTAAATCA
GATGATATGATTGGACGTAATAAAGTGTATTATGCAATTACACACGGCCAAGAAATACCA
AAAGCAAGTATTCCTGAATCCTTTAGAGTATTAACAAGAGAACTTCAAGCCCTAGGCTTA
TATGTAGAACTCATTGATGCAAACAAGGGAGAGAATGAGGCGATCAAGTCTTTAGTAGAC
AACTCGTCCAAAGGTTTTAACAAAGGGAGGTTTTAA

>protein sequence
MSYKTVKYGKKAERRDYSKMLHQVELPNLIEIQTKSFEEFVETGIDELLKDISPIEGHNG
DLKLYFEESSLSEPKYSTIETKIRDLNYSRQLSARVKLENAITGEVRESTVLMTDLPMMT
PSGTFIINGAERVVVSQIVRSSGVYYTSELDKKINQIKYSAQVIPTRGAWIEFEQGSKEI
LYAKLDRSKKVPLTTFIRALGFTTKKDIEETFGRSSLITNTFEKDETKSPNEAVIDLYSK
LRQGEKVPADAAREFIRMRLFDARRYDLAKVGRYKFIKKLDVLARAEGTYLANDIILDGE
VLVAKDTHLTKEVIQILAQNRDAFRKQLITKENNLQNETADEILATTLPEGGNTLYAKEN
VVNLKTGEVLVKKNEAITEEVLTNLRKNRHSIDEKVIKYFLTEDVYKKESLRQGVISEIL
DVYVYDDAGDKSNVIRIIGSDQREDRIFVAVSDIVASISYFLNLYDGLGNVDDIDHLGNR
RLRLIGELLKNQFRIGLARAEKNIKDKMSTTNFNDATPANVINMTPLVGAIKTFFGSSQL
SQFMDQINPLAELTQKRRVSALGTGGIARDRAGVEVRDVHNSHYGRLCPIETPEGPSIGL
ISSLATYAKVDEYGFIKTPFLKVVQNGNETSVTKEVIYLTADEESDHVIASAATPMDEHG
LFIESRVIARRNGETGIYDANEITAMDVSPKQVVSVATSSIPFLEHDDASRALMGANMQR
QAVPLLQPTSPIVGTGVEYRAAKDSGAVVIAKNSGYVTYVDGKKIIITPEPTDTIKSGSK
TLYKVGGEFDYGIAKKLYETKSVQNDQYDLVQFFRSNQDTLILQKPIVMQGEYVDAGDVI
ADGPSTENGELALGRNVTVAFMTWEGYNYEDAVIMSEDLVKHDVYTSIHIDEYEIETRDL
KQANGKEEITREIPNVGQEAIKFLDERGIIVPGSEVKEGDILVGKITPKGVFEPTPSEKL
IHAVIGDKAKEYRDSSLRVPHGGGGVVQSVQYFSKKNGDQLPTGVNEQIRVYIAKKRKIT
EGDKMAGRHGNKGVISKILPREDMPYLADGTYVDIMLNPLGVPSRMNIGQILEIHLGISA
QKLGIKVASPVFDGVTNDELGAIMEEAGIAPDGKTTLYDGRTGEPYNNRISVGVMYMIKL
SHMVDDKLHARNVGPYTLVTQQPMGGKAQNGGQRFGEMEVWALYAYGAAHTLQEMLTIKS
DDMIGRNKVYYAITHGQEIPKASIPESFRVLTRELQALGLYVELIDANKGENEAIKSLVD
NSSKGFNKGRF