ACL_RS00845

Uniprot: A9NEL8
Description: DNA-directed RNA polymerase subunit beta'
EC number: 2.7.7.6
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. {ECO:0000250}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): rpoC
Gene names (synonym):
Mass: 155,620
Subunit structure [CC]: SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity). {ECO:0000250}.
Gene ontology (GO): DNA binding [GO:0003677]; DNA-directed RNA polymerase activity [GO:0003899]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003899; GO:0006351
Chain: CHAIN 1 1375 DNA-directed RNA polymerase subunit beta'. /FTId=PRO_0000353468.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RNA polymerase beta' chain family. {ECO:0000305}.
Protein families: RNA polymerase beta' chain family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 1 158 Unknown.; REGION 159 1353 DNA-directed RNA polymerase subunit beta'.
EMBL: CP000896
ProteinModelPortal: A9NEL8
MEROPS:
EnsemblBacteria KO: ABX80798
UniPathway: K03046
CDD:
Gene3D:
HAMAP:
InterPro: MF_01322
PANTHER: IPR012754;IPR000722;IPR006592;IPR007080;IPR007066;IPR007083;IPR007081
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF04997;PF00623;PF04983;PF05000;PF04998
SMART:
SUPFAM: SM00663
TIGRFAMs:

158357-162485(+)

>nucleotide sequence
ATGGCTAAGAACGAAGTATTATCCTTACCTGTAGATTCTTTAAAATTAAGTCAAACTGCA
TTAGATAGATTAAAACTATCTGGTATTAGTCGTTTAGAAGATTTCAATACATTTAACTTA
AAAGAACTTCAAATGTTATTGAGTGATTCTTTTAATGAAGTCCTTCCAACATTAATTTAT
TACAAATTACCAAGAGATGTAAGAGATTTATCATTATCAGATGAAGTTGTTTCAGTACTT
GAAACAGCAGGTATTAAAGATTTAGAAGCATTAACAAAATATGACAAATCTACACTTTAT
CATGTGTTTAAAGACGATGAGTTCTTATTAAAAGAAATCAACGATTTATTTGAACTATAT
CACGAAGAAAAATTATCTACTCTTAATGTAGCAACAGAAGTTTTTGAAGAAGTAGCACTT
CCTCAAGAGGTATCTGTTGAAGACAGAGTAAATAAGATCATTCAACCAATTCGTAAAACA
TATGGTTCTAAAGCATTTACGCACTTTAAAGTGAGACTTGCAAGTCCAGATGAAATTAGA
AACTGGTCTTATGGTGAAGTTATTAACCATGAAACTATTAACTATAGAACATCAAAACCT
GAACCAGGTGGACTATTTGATGAAAGAATTTTTGGACCTACAAGAGATTACCAATGTGCT
TGTGGTAAGAAACAAACTGTAAACAAAGGCCAAATTTGTCCAAAATGTGGTATTGAAATT
ACAGAGTCTAAAGTACGTCGTGAACGTATGGGACATATTAACTTAGAAGCACCTGTTGTG
CATACATGGTACTTAAAGAACTCTCCTTCAAGATTAGCTATCCTATTAGGCATTAAAGCT
AAACAATTAGAAGAAGTTGTTTACCATGCAAGTTATATTGTGACTGACCCAGGTCAAAAC
ACACCACTAGCTAAAAAACAAATCTTATCCGAACAAGACTATTCTTTACTTGTTGAAGAA
TATGGTTCAAGATTTACAGCGCTTACTGGTGCTGAAGCGGTTAAAAAATTACTTCAAGAT
CTAGATTTAGATAAAGAAGTTAAAAATTTAAGAAAACGTCTTAAAACAAGTTCTAAACAA
AAACGTGATCGTATTATTAAACGTTTAGATGTTGTTGAAGCATTTAACCAATCAGACAAT
AAACCTGAGTGGATGGTTATGGATGTTATTCCAGTCATTCCACCAGATTTACGTCCAATG
GTTCCACTAGATGGTGGTAGATTTGCTACAACTGACCTTAACGACTTATATAGAAGAATC
TTAAACAGAAACAACCGTTTAAAGAAACAAAAAGAACAAATGGCGCCTCGTTTAATTACT
AAGAACGAAAAGCGTATGTTACAAGAAGCAGTAGATGCATTATTCGATAACGCGAAACGC
GGTAAGAAGGCTGCTGTTGAACGTAACCGCCACTTAAAATCATTATCTGATTTACTACGT
GGTAAACAAGGTCGTTTCCGTCAAAACTTATTAGGAAAACGTGTGGACTACTCTGGACGT
TCAGTTATTATTGTTGGTCCAGACTTAGAGATGTACCAATGTGGTATTCCTCGTGAAATG
GCAATTACATTATTTAAACCATTTATCTTACGTGAATTACAACTAACACATGGTGCAGAA
AAGAAAAATGCAAACGCTAAATACGAAAGACGCGATGACGACACATGGCGTGCACTTGAA
AAAGTTGTACGTGAGCACCCAGTATTACTAAACCGTGCGCCAACGCTACATAGACTTGGT
ATTCAAGCATTCGAAGTGAAATTAATTGATGGTAAAGCGATTCGTCTACACCCACTTGTT
ACACCTGCGTTTAACGCGGACTTTGACGGGGACCAAATGGCGGTTCACTTACCACTATCA
CCAGAAGCTCAAGCAGAAGCAAGATTATTAATGCTTGCATCTAACAACATCTTAAACCCT
AGAGATGGTAAACCAGTTGTTACACCATCTCAAGATATGGTTTTAGGTAACTATTACCTA
ACTATTGAAGAATCTAAAGATCGTAATTTTGGTGATGATCTTGAAAGAACTAAGAAACAC
CAAGAAAAGCATCGCCATGAAGGTAAGTTCTTCTCATCTATTGATGAAGTTAAGATTGCT
TATGAGAATAAAGATATTTCTTTACATACAAGAATTATCATTAAACCAGAATCAGTTAAA
GACACATTTACAGTAGAACAAAAGAACATGTATTTAGTGACAACATTAGGTAAAATCATC
TTTAACGAAATCTTACCTGAAACATTCCCTTATGTAAATGAACCTACAATGTCTAACTTA
AGTGAAAAGACACCAGACATTTACTTTATTAAAAAGGGTGTGAATCCAAAGGATGCACTA
AAGCATATTCCTACACCTGAGCCATTTAAGAAGAGATTCTTATCTATGGTTATTGCTCAA
GTATTTAAATTATTCCACATTTCTGAAACATCAAGAATGTTAGATAAATTAAAAGACTTA
GGATTTAAATATTCAACAGTTGCTGGTATTACAATTTCTTATGCAGACATCAACGTATAC
TCCAAGAAAAAAGAAATGGTTGAAGCAACCGAAGAAGAAATTAATGAAATTGAAGAATGG
TTCGAAGATGGATTACTAACAGATTCAGAACGTCGTAAACTTGTTATTGATAAATGGACA
AATGTTAAAAACGAAATTCAATCTGGTATTATGAAAGAATTCGATAAAGATAACAACATC
TTTATGATGAGTGACTCTGGTGCCCGCGGTAACGTATCTAACTTTACACAGTTAGTCGGT
ATGAGAGGACTTATGAGTAACCCTAAAGGGGAAACGATTGAAGTTCCAGTTCAATCATCA
TTTAGAGAAGGTCTAACCGTATCTGAATTCTTTATCTCCACCCACGGTGCGAGAAAAGGT
TCTACCGATACCGCGCTTAAGACCGCAGAATCTGGTTACTTAACACGTCGTTTAGTCGAC
GTATCACAAGACGTTATTATTGTTGAGGATGATTGTGGTTCAAGCCATGGTGTTTATGTA
GAAGCAATTAAAGATGAGTCAGGTAAAGAAATCGTTCCATTATACGATAGAATTTATGGC
CGCTTTGCTGCACATGACATTATCAGTCCAAAAACTGGTGAAGTATTTGTGAAACGTAAT
GAACTGATTACTGAAGAAATCGGTTTGGCAATCGTTAAATCAGGTATGCAAAAAGTTGAA
ATTAGAAGTATTATGACATGTACATCAAGTCATGGTATTTGTGCGAAAGACTACGGTATT
AACTTAGCTACAAACCAATTTGTTGAAGTTGGTGAAGCTATTGGGGTTGTTGCTGCTCAA
TCTATTGGTGAGCCAGGTACACAGTTAACCATGCGTACATTCCATACAGGTGGGGTTGCT
GCTGGTGCCGATATTACTCAAGGTTTACCACGTATTCAAGAGTTATTTGAAGCACGTAAT
CCAAAAGGTAAAGCAACGATTTCAGAAGTTGAAGGTAAAGTTAAAGATATCTCACGTCGT
GGTGGTTCAATTAGCATTACAATTACTGATACCCAAGGCACTGAATACAAATACACGCTT
GATCCAAACATTGAAGCTTTAGTTAAAAAAGGTCAAGATGTTGTTGCAGGTCAAAAACTA
TCTAGTGGATCTATTAATCCAAAAGAATTACTACGTGTAACAGATGTTAAAACAGCATCT
AACTACATTTTAGAAGAAGTTCAAAAAGTTTACCGCGCTCAAGGGGTTGAAATTTCTGAC
AAGCACGTTGAAGTTATTATTCGTCAAATGTTACGTCGTATCATGGTAATTATTGAAGGT
GATACCAACATCTTACCAGGTACAGAGGTATCTATTGATGAGTTTAAACGTGAAAATAAA
GAAGTACTTAAAAACCGTGGTCGCTTAGCTGTTGGTCGCCCAATACTATTAGGTATTACT
AGAGCATCTCTAAGAAGTGATTCCTTCTTATCTGCTGCATCCTTCCAAGAAACTACAAGA
ATCTTAACAGATGCTGCAATTAGAAGTAAGAAAGATGAATTACACGGACTAAAAGAAAAC
GTCATTATCGGTGGATTAATCCCAGCTGGTACAGGTATACTGCAAGAAAAATTCTTCCAT
TATGATCAACCAGAAGATAAAAAACCAATATATGATGATTTTGAATAA

>protein sequence
MAKNEVLSLPVDSLKLSQTALDRLKLSGISRLEDFNTFNLKELQMLLSDSFNEVLPTLIY
YKLPRDVRDLSLSDEVVSVLETAGIKDLEALTKYDKSTLYHVFKDDEFLLKEINDLFELY
HEEKLSTLNVATEVFEEVALPQEVSVEDRVNKIIQPIRKTYGSKAFTHFKVRLASPDEIR
NWSYGEVINHETINYRTSKPEPGGLFDERIFGPTRDYQCACGKKQTVNKGQICPKCGIEI
TESKVRRERMGHINLEAPVVHTWYLKNSPSRLAILLGIKAKQLEEVVYHASYIVTDPGQN
TPLAKKQILSEQDYSLLVEEYGSRFTALTGAEAVKKLLQDLDLDKEVKNLRKRLKTSSKQ
KRDRIIKRLDVVEAFNQSDNKPEWMVMDVIPVIPPDLRPMVPLDGGRFATTDLNDLYRRI
LNRNNRLKKQKEQMAPRLITKNEKRMLQEAVDALFDNAKRGKKAAVERNRHLKSLSDLLR
GKQGRFRQNLLGKRVDYSGRSVIIVGPDLEMYQCGIPREMAITLFKPFILRELQLTHGAE
KKNANAKYERRDDDTWRALEKVVREHPVLLNRAPTLHRLGIQAFEVKLIDGKAIRLHPLV
TPAFNADFDGDQMAVHLPLSPEAQAEARLLMLASNNILNPRDGKPVVTPSQDMVLGNYYL
TIEESKDRNFGDDLERTKKHQEKHRHEGKFFSSIDEVKIAYENKDISLHTRIIIKPESVK
DTFTVEQKNMYLVTTLGKIIFNEILPETFPYVNEPTMSNLSEKTPDIYFIKKGVNPKDAL
KHIPTPEPFKKRFLSMVIAQVFKLFHISETSRMLDKLKDLGFKYSTVAGITISYADINVY
SKKKEMVEATEEEINEIEEWFEDGLLTDSERRKLVIDKWTNVKNEIQSGIMKEFDKDNNI
FMMSDSGARGNVSNFTQLVGMRGLMSNPKGETIEVPVQSSFREGLTVSEFFISTHGARKG
STDTALKTAESGYLTRRLVDVSQDVIIVEDDCGSSHGVYVEAIKDESGKEIVPLYDRIYG
RFAAHDIISPKTGEVFVKRNELITEEIGLAIVKSGMQKVEIRSIMTCTSSHGICAKDYGI
NLATNQFVEVGEAIGVVAAQSIGEPGTQLTMRTFHTGGVAAGADITQGLPRIQELFEARN
PKGKATISEVEGKVKDISRRGGSISITITDTQGTEYKYTLDPNIEALVKKGQDVVAGQKL
SSGSINPKELLRVTDVKTASNYILEEVQKVYRAQGVEISDKHVEVIIRQMLRRIMVIIEG
DTNILPGTEVSIDEFKRENKEVLKNRGRLAVGRPILLGITRASLRSDSFLSAASFQETTR
ILTDAAIRSKKDELHGLKENVIIGGLIPAGTGILQEKFFHYDQPEDKKPIYDDFE