ACL_RS00965

Uniprot: A9NEP3
Description: replicative DNA helicase
EC number: 3.6.4.12
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. {ECO:0000256|RuleBase:RU362085}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. {ECO:0000256|RuleBase:RU362085}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): dnaB1
Gene names (synonym):
Mass: 49,887
Subunit structure [CC]:
Gene ontology (GO): primosome complex [GO:1990077]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA helicase activity [GO:0003678]; DNA replication, synthesis of RNA primer [GO:0006269]
Gene ontology IDs: GO:0003677; GO:0003678; GO:0005524; GO:0006269; GO:1990077
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the helicase family. DnaB subfamily. {ECO:0000256|RuleBase:RU362085}.; SIMILARITY: Contains 1 SF4 helicase domain. {ECO:0000256|RuleBase:RU362085}.
Protein families: Helicase family, DnaB subfamily
Coiled coil:
Domain [FT]: DOMAIN 174 442 SF4 helicase. {ECO:0000259|PROSITE:PS51199}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEP3
MEROPS:
EnsemblBacteria KO: ABX80823
UniPathway: K02314
CDD:
Gene3D: cd00984
HAMAP: 1.10.860.10;3.40.50.300
InterPro:
PANTHER: IPR007692;IPR007694;IPR007693;IPR016136;IPR027417
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51199
ProDom: PF00772;PF03796
SMART:
SUPFAM:
TIGRFAMs: SSF48024;SSF52540

189385-190720(+)

>nucleotide sequence
ATGGCCAATAAAATTCCTAACGCAGTAGATGCTGAAAAATCAGTATTAGGGGCTATTCTT
TTAGATAGTCAAACTGCACCGAATATTTTTGATGAGATCTCCGAAAGTGATTTTTACTTA
AATGCACATGGACTTATCTTTCAAGCAATGAAGGATTTGTATAATGATCGTAAATCGATT
GACTACACATCATTAAAATCAATCTTAGAGCATAAATCTCAATTAGAAACTGTTGGTGGA
TTATCTTATATTCTTGATTTATCAGAATATACTGTATCCATTGACCATATTGATACCTAT
ATTGAAATTGTTAAAGACTTATCCTTAAAAAGAGATGTTATTAGAATTACACAAGAGCTT
GCTACTCAAGGTCTTACATCAGATATTAACTCTGCTGAATACTTAGATAAAGTTGAAGGT
GCAATCTTAAGTTTATCTCAAAGAAGAAAAGTTGGAGCATTTAAAAATATCGAAGATATT
GTTGGCGATGTCACCGAAAAATTACATCACTTACAAAATCAAAAAGGTGAAGTAACAGGT
TTAAGAACTGGTTATGCTACATTAGATAAATATACGAATGGGCTTCAACCAGAAGAACTT
ATTATCTTAGCTGCTAGACCTGCTGTTGGTAAATCAGCGTTTGCGATGAACCTAGCTTTA
AATGCTGCTAAGTATAATAAAGGCGGTAAAGCGGGCGTTGCAATTTTCTCTCTTGAAATG
AGTAATGAACAATTAGTAACCCGTATGATAGCAAGTATGTCTCATATAGAAAACTCTAAA
TTAAGAACAGGTTTTTTAACACCACAAGAATGGCGTAACTATGAGAATATGACAGGTATT
TTAAATGACTATAATATCTATTTTGATGATTCATCTTCATCAAATATCAATGATATTAGA
GCAAAATGTCGCCGTCTTGCTCAAGAAGGTAAATTAGATTTTGTTGTGATTGACTATCTT
CAATTAATTCATGCTGATGGTAATAACAGACAAGAAGAAGTTTCTAAAATCTCTAGAGCT
TTAAAACAAATGGCTAAAGAATTACGTGTTCCGGTACTGGCATTAAGTCAGCTTTCTCGT
GATGTTGAAAAATCAACGGATAAGCGTCCAACGCTTGCGCACTTAAGAGAATCTGGTTCG
ATCGAGCAAGATGCCGATATTGTTATGTTTATTCATAGAGAAGAATACTATGCAGCATCA
AGTGATGGTGAACAAACAGGACAAACAGAAATCTTGGTGCGTAAAAACCGTCAAGGACGT
ATTGGTGAAATTAACTTTATCTTCTCACCTCAATACTCAAGATTTGATGAACAATCCAAT
ATTGAAGAAGCTTAA

>protein sequence
MANKIPNAVDAEKSVLGAILLDSQTAPNIFDEISESDFYLNAHGLIFQAMKDLYNDRKSI
DYTSLKSILEHKSQLETVGGLSYILDLSEYTVSIDHIDTYIEIVKDLSLKRDVIRITQEL
ATQGLTSDINSAEYLDKVEGAILSLSQRRKVGAFKNIEDIVGDVTEKLHHLQNQKGEVTG
LRTGYATLDKYTNGLQPEELIILAARPAVGKSAFAMNLALNAAKYNKGGKAGVAIFSLEM
SNEQLVTRMIASMSHIENSKLRTGFLTPQEWRNYENMTGILNDYNIYFDDSSSSNINDIR
AKCRRLAQEGKLDFVVIDYLQLIHADGNNRQEEVSKISRALKQMAKELRVPVLALSQLSR
DVEKSTDKRPTLAHLRESGSIEQDADIVMFIHREEYYAASSDGEQTGQTEILVRKNRQGR
IGEINFIFSPQYSRFDEQSNIEEA