ACL_RS01070

Uniprot: A9NER3
Description: 3-dehydroquinate synthase
EC number: 4.2.3.4
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. {ECO:0000256|HAMAP-Rule:MF_00110, ECO:0000256|SAAS:SAAS00210910}.
Cofactor: COFACTOR: Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000256|HAMAP-Rule:MF_00110}; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00110}; ; Note=Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+). {ECO:0000256|HAMAP-Rule:MF_00110};; COFACTOR: Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000256|HAMAP-Rule:MF_00110, ECO:0000256|SAAS:SAAS00210873}; ; COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|SAAS:SAAS00607009};
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. {ECO:0000256|HAMAP-Rule:MF_00110, ECO:0000256|SAAS:SAAS00210866}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): aroB
Gene names (synonym):
Mass: 37,464
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00110, ECO:0000256|SAAS:SAAS00210852}.
Gene ontology (GO): cytoplasm [GO:0005737]; integral component of membrane [GO:0016021]; 3-dehydroquinate synthase activity [GO:0003856]; aromatic amino acid family biosynthetic process [GO:0009073]; chorismate biosynthetic process [GO:0009423]
Gene ontology IDs: GO:0003856; GO:0005737; GO:0009073; GO:0009423; GO:0016021
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the dehydroquinate synthase family. {ECO:0000256|HAMAP-Rule:MF_00110, ECO:0000256|SAAS:SAAS00534509}.
Protein families: Dehydroquinate synthase family
Coiled coil:
Domain [FT]: DOMAIN 61 308 DHQ_synthase. {ECO:0000259|Pfam:PF01761}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NER3
MEROPS:
EnsemblBacteria KO: ABX80843
UniPathway: K01735
CDD:
Gene3D:
HAMAP:
InterPro: MF_00110
PANTHER: IPR016037;IPR030963;IPR030960
PIRSF:
PRINTS: PIRSF001455
PROSITE:
Pfam:
ProDom: PF01761
SMART:
SUPFAM:
TIGRFAMs:

209219-210218(+)

>nucleotide sequence
ATGAGACAAACACTCGATAATTATGAAATAGTAGTTAAACAAAATTTGATGAATGATTTG
GGTGAGGAAATAACTAGTGTTTATCCTTATAAAGATATTTTTGTTATTACTGACCATAAT
GTATTTAAACTATATGAAAACTTATTAACAACGAAGTTAAATATGTTTAATTTAAAATTT
GTAAGTGTTGAACCAGGCGAACAATCAAAATCTTTAAGCACCTACCAAGAAGTTGTTCAA
AAATTACTTAAATTAGGTATGAGAAAGAATCATTTACTTATTGCCTTAGGTGGTGGTGTA
GTAGGAGATTTAGCTGGATTTGTTGCAGCTACTTTATTTAGAGGTGCACCATTTATTCAA
ATACCAACAACGTTACTAGCGATGGTAGATTCATCAATTGGCGGTAAAACTGGTCTAGAT
TTAAATGAAGGTAAAAATTTAATTGGTGCTTACAAAAATCCACTTAAAGTTTTAATTGAT
CCTTCATTTTTAGATACATTACCTAAAGTAGAATATAAAAATGGATTAGCAGAAGTATTA
AAACATGGACTCATTGGGGATCCTAAATTATATGAATATTTAAAAGTAAATGATAAATTA
ACACTGAATGAAATCAAACGTGCAATTCAAGTCAAAGTGAACATTGTTAAAAAAGATCCG
TTTGAACAAAACGAACGTATGTTCTTAAATTTTGGACATACCTTTGGACATGCAATCGAG
CGTCATTATGATTTTGCAATCAAACACGGTGTAGCAATTAGCTATGGTATGTTAATTAGT
TTAGAATTAGGCATTAAAGCAAAGATTACACCAGCACATCTTTTTGATGATGTTAAAAAC
ATTTTATTAAGATTAGAACTAGTTAAAGAACCTTTATTAGATAAAAAACAATTTATAGAA
TATATAGGTTTTGACAAGAAAAACCTAGCGGATGGTTTACGTTTTGTTTATATCAAAAAC
ATAGGTGAACCAATCATTAAGAAAGGTGTTGAAATATAA

>protein sequence
MRQTLDNYEIVVKQNLMNDLGEEITSVYPYKDIFVITDHNVFKLYENLLTTKLNMFNLKF
VSVEPGEQSKSLSTYQEVVQKLLKLGMRKNHLLIALGGGVVGDLAGFVAATLFRGAPFIQ
IPTTLLAMVDSSIGGKTGLDLNEGKNLIGAYKNPLKVLIDPSFLDTLPKVEYKNGLAEVL
KHGLIGDPKLYEYLKVNDKLTLNEIKRAIQVKVNIVKKDPFEQNERMFLNFGHTFGHAIE
RHYDFAIKHGVAISYGMLISLELGIKAKITPAHLFDDVKNILLRLELVKEPLLDKKQFIE
YIGFDKKNLADGLRFVYIKNIGEPIIKKGVEI