ACL_RS01090

Uniprot: A9NER7
Description: chorismate synthase
EC number: 4.2.3.5
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate. {ECO:0000256|HAMAP-Rule:MF_00300}.
Cofactor: COFACTOR: Name=FMNH2; Xref=ChEBI:CHEBI:57618; Evidence={ECO:0000256|HAMAP-Rule:MF_00300}; ; Note=Reduced FMN (FMNH(2)). {ECO:0000256|HAMAP-Rule:MF_00300}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system. {ECO:0000256|HAMAP-Rule:MF_00300}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7. {ECO:0000256|HAMAP-Rule:MF_00300}.
Active site:
Binding site: BINDING 46 46 NADP. {ECO:0000256|HAMAP-Rule:MF_00300}.; BINDING 254 254 FMN; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00300}.; BINDING 296 296 FMN. {ECO:0000256|HAMAP-Rule:MF_00300}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 123 125 FMN. {ECO:0000256|HAMAP-Rule:MF_00300}.; NP_BIND 269 273 FMN. {ECO:0000256|HAMAP-Rule:MF_00300}.
Site:
Gene names (primary): aroC
Gene names (synonym):
Mass: 35,720
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00300}.
Gene ontology (GO): chorismate synthase activity [GO:0004107]; aromatic amino acid family biosynthetic process [GO:0009073]; chorismate biosynthetic process [GO:0009423]
Gene ontology IDs: GO:0004107; GO:0009073; GO:0009423
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the chorismate synthase family. {ECO:0000256|HAMAP-Rule:MF_00300}.
Protein families: Chorismate synthase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NER7
MEROPS:
EnsemblBacteria KO: ABX80847
UniPathway: K01736
CDD:
Gene3D: cd07304
HAMAP: 3.60.150.10
InterPro: MF_00300
PANTHER: IPR000453;IPR020541
PIRSF: PTHR21085
PRINTS:
PROSITE:
Pfam: PS00788
ProDom: PF01264
SMART:
SUPFAM:
TIGRFAMs: SSF103263

212483-213470(+)

>nucleotide sequence
ATGAATAGTTTCGGCACTTTATTTAAAGTAACTTTATATGGTGAATCACATCAAGATGCT
ATAGGTATAGTTATTGATGGTGTAAAACCCGGTCTTAAAATTAACTGGGATGATGTAGAA
GGCGACCTAGCTAAAAGAAGACCAGGTGCTGTAGGTACAACACCAAGAGTTGAAAAAGAT
AAACTAGAAGTTACATCTGGTATATTTAATGAATATACTACAGGTTCTCCACTACATATT
ATGATCAGAAATGAAAATGTTATTTCTAAAGACTATAGTCATCTTAAAAGTCAACCAAGA
CCAGGACATGCAGACTTTGTAGCTAGTATGAAGTATCAAGGATTTCATGACTACCGAGGT
GGGGGTCGTTTTTCTGGAAGACTAACTGCACCACTTGTGGTTGCAGGTAGTATTGCAAAA
CAAATGTTACCGTTTGAGTTTAGTCATGAACTGATTCAAGTGGGATCTCTTACAGATATG
TCACAACTCGATACATATTTACGTGAAGTACATAACCAAGGAGAATCTGTTGGTGGCATC
GTTGAAGTTCGAGTAAAAAATATGATAGTTGGACTAGGCGAACCTATGTTTGAAAAGTTA
GATGCTAAAATTGGTCAAATGATGTTTTCAATACCGGCTGTAAAAGGTGTTGAAATTGGT
ACAGGTTTTAGTGGCGCAACTCTCTATGGTTCTGAGTTTAATGATGTCTATGAAGATGAA
ACCGGAAAGACTTTAACCAATCATTCTGGTGGAGTATCTGGTGGTATTTCTAATGGTAAT
GATTTAGTCGTAAAGGTTTTTGTTAAGCCAACTAGCAGTATTGGTAAAGCACAATCTTCA
TTCAATTTAGAAAGTAAACAAAAACAGTCTTTTGAAATTGGTGGTAGACATGATACTGCA
ATTGTAAGACGTGTAGGTATTGTACTAGAAAATGCACTTGCTATTGTATTAGCAGACCTA
TATTTATGGAGTAAAATATATAAATGA

>protein sequence
MNSFGTLFKVTLYGESHQDAIGIVIDGVKPGLKINWDDVEGDLAKRRPGAVGTTPRVEKD
KLEVTSGIFNEYTTGSPLHIMIRNENVISKDYSHLKSQPRPGHADFVASMKYQGFHDYRG
GGRFSGRLTAPLVVAGSIAKQMLPFEFSHELIQVGSLTDMSQLDTYLREVHNQGESVGGI
VEVRVKNMIVGLGEPMFEKLDAKIGQMMFSIPAVKGVEIGTGFSGATLYGSEFNDVYEDE
TGKTLTNHSGGVSGGISNGNDLVVKVFVKPTSSIGKAQSSFNLESKQKQSFEIGGRHDTA
IVRRVGIVLENALAIVLADLYLWSKIYKW