ACL_RS01120

Uniprot: A9NES4
Description: ribonuclease III
EC number: 3.1.26.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphomonoester. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231310}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231322};
Enzyme regulation:
Function [CC]: FUNCTION: Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism. {ECO:0000256|HAMAP-Rule:MF_00104}.
Pathway:
Active site: ACT_SITE 44 44 {ECO:0000256|HAMAP-Rule:MF_00104}.; ACT_SITE 111 111 {ECO:0000256|HAMAP-Rule:MF_00104}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 40 40 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 108 108 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.; METAL 111 111 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00104}.
Nucleotide binding:
Site:
Gene names (primary): rnc
Gene names (synonym):
Mass: 25,016
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00104, ECO:0000256|SAAS:SAAS00231419}.
Gene ontology (GO): cytoplasm [GO:0005737]; metal ion binding [GO:0046872]; ribonuclease III activity [GO:0004525]; rRNA binding [GO:0019843]; mRNA processing [GO:0006397]; rRNA catabolic process [GO:0016075]; rRNA processing [GO:0006364]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0004525; GO:0005737; GO:0006364; GO:0006397; GO:0008033; GO:0016075; GO:0019843; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain. {ECO:0000256|HAMAP-Rule:MF_00104}.; SIMILARITY: Contains 1 RNase III domain. {ECO:0000256|HAMAP-Rule:MF_00104}.
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 1 122 RNase III. {ECO:0000259|PROSITE:PS50142}.; DOMAIN 146 215 DRBM (double-stranded RNA-binding). {ECO:0000259|PROSITE:PS50137}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NES4
MEROPS:
EnsemblBacteria KO: ABX80854
UniPathway: K03685
CDD:
Gene3D: cd00593
HAMAP: 1.10.1520.10;3.30.160.20
InterPro: MF_00104
PANTHER: IPR014720;IPR011907;IPR000999
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50137;PS00517;PS50142
ProDom: PF00035;PF14622
SMART:
SUPFAM: SM00358;SM00535
TIGRFAMs: SSF69065

220411-221071(+)

>nucleotide sequence
ATGAATGATTTATTGAAACAACTTAATATTGAAACAAAACATATAGATTTATACCTTCAA
GCATTAACGCATACATCTTATGCGCATGAAAACCAAACAGATCATAATGAACGATTAGAG
TTTTTAGGTGATGCAGTGATTGAATTAATGATGAGTACATACCTATTTACTCATGATCAA
TCCAATCAAGGTGTTTTAACTAAAAGACGTGCTCAAGCAGTTCGTGAAGAAGCACTTGTT
TTGTATGCCCAAAAAATGGATTTAGAATCGAAGATGCGTTTAGGCAATGGTGAGCAAATA
GCAAAACCAAGCATGATCGCCGATGCATTTGAAGCGTTTTTTGGTGCACTATATATGGAT
CAAGGATTTGATAAGACTTATGAAATCTTTAATAAGGTAGTCATACCACATTTAAATTTA
GTGGAGCACATTAAAGACTATAAAACTCAACTTCAAGAATTTATTCAACTTGAAAGGAAA
AGTTTATCTTATAAAACAGTTAAAGTGGGTGGACCATCACATCGCCCAATATTTAAGTCT
GAAGTATTTTTAGAAAACAGTATTATGTTGGGCACAGGACAAGGTAGTTCCACCAAGGAA
GCCGAACAAAATGCTGCAAAAGAAGCACTAAGTAAAGTAGTAAAGGAGCGTAGTTTATGA

>protein sequence
MNDLLKQLNIETKHIDLYLQALTHTSYAHENQTDHNERLEFLGDAVIELMMSTYLFTHDQ
SNQGVLTKRRAQAVREEALVLYAQKMDLESKMRLGNGEQIAKPSMIADAFEAFFGALYMD
QGFDKTYEIFNKVVIPHLNLVEHIKDYKTQLQEFIQLERKSLSYKTVKVGGPSHRPIFKS
EVFLENSIMLGTGQGSSTKEAEQNAAKEALSKVVKERSLW