ACL_RS01190

Uniprot: A9NET8
Description: leucine--tRNA ligase
EC number: 6.1.1.4
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). {ECO:0000255|HAMAP-Rule:MF_00049}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site: BINDING 584 584 ATP. {ECO:0000255|HAMAP-Rule:MF_00049}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): leuS
Gene names (synonym):
Mass: 92,810
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; aminoacyl-tRNA editing activity [GO:0002161]; ATP binding [GO:0005524]; leucine-tRNA ligase activity [GO:0004823]; leucyl-tRNA aminoacylation [GO:0006429]
Gene ontology IDs: GO:0002161; GO:0004823; GO:0005524; GO:0005737; GO:0006429
Chain: CHAIN 1 803 Leucine--tRNA ligase. /FTId=PRO_0000334722.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. {ECO:0000255|HAMAP-Rule:MF_00049}.
Protein families: Class-I aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]:
Motif: MOTIF 41 52 "HIGH" region.; MOTIF 581 585 "KMSKS" region.
Region:
EMBL: CP000896
ProteinModelPortal: A9NET8
MEROPS:
EnsemblBacteria KO: ABX80868
UniPathway: K01869
CDD:
Gene3D:
HAMAP: 1.10.730.10;3.40.50.620;3.90.740.10
InterPro: MF_00049_B
PANTHER: IPR002302;IPR025709;IPR013155;IPR015413;IPR014729;IPR009080;IPR009008
PIRSF: PTHR11946:SF7
PRINTS:
PROSITE: PR00985
Pfam:
ProDom: PF08264;PF13603;PF09334
SMART:
SUPFAM:
TIGRFAMs: SSF47323;SSF50677

237000-239412(+)

>nucleotide sequence
ATGCATAAATATGAACACAGAAAAATAGAAGAAAAATGGCAAAAACATTGGTTAGACAAT
AAGTTGTTTAAAACCAAAAACGATAGATTTCATCAGAAATACTATGTTTTAGATATGTTT
CCATACCCTTCGGCCTCAGGACTACATGTAGGGCACATTGAAGGATATACAGCAACAGAT
ATCGTGTCACGTTTTAAACGTATGCAAGGATATAATGTATTACATCCAATGGGATGGGAT
GCATATGGGTTACCTTCTGAGCAATATGCGCTCGCAACCGGAAAAGATCCAAAATCTTTC
ACATACCAAAATATTAGTAATTTCAAAAGACAAATTATTGAAATGGGTAAAGGTGTAGAT
TGGGATAAAGAGCTAGCTACAACAGACCCTGAGTATTTTAAATGGACTCAGTGGATTTTT
AAACGCCTTTATGAGAAGGGTTTAGCGGTCTTAAAAGATGTTGAAGTCAACTGGTGTGAA
GGTTTAGGTACAGTACTTGCTAATGATGAAATTGAAATCATTAATGGACAAATGGTATCC
GAACGCGGACATTACCCAGTTATTAAAAAACCAATGCGTCAATGGGTATTAAGAATCACT
GAGTATGCAGATCGACTACTTGATGATCTTGCTTTAGTGGATTGGCCTGAAAACCTAAAA
GAAATGCAACGCAACTGGATTGGTAAATCCAGTGGTGCCGTGATTGAATTTGAAGTGGCA
TCCACAGATTATAAATTTGAAATATTTACTACAAGACCTGATACTATCTACGGTGTGACT
TACTGTGTACTTTCCCCAGAACACCCACTAGTAGAAAAAATTGTGACTCAAGATGAAGAA
AAAGATGTATTAAGATACATTGAACAAACTAAAATGAAGTCAGATTTAGATCGTATTGCA
GATAAATCTAAAACAGGTGTGTTTACTGGTGCTTTTGCAAAACACCCACTAACAAACCAA
TTAATTCCAATTTGGATTGGTGACTATGTACTACCTCAATATGGGATGGGCGCTGTTATG
GCAGTACCAGCACATGATGATAGAGACTATGAGTTTGCTATGCAATATGGTTTAAACATT
ATTGAAGTTATTAAAGGTGGTAGCGAAGGTGCTTATACTGGAGACGGTATCCATCATCAC
TCAGGCATTATTGATGGTTTATATAATGAAGAAGCTATTGAAAAAATAACTAAACATTTA
GAAGATCGCAAAAAAGGGTACAAACACTACACTTATAAGTTAAGAGACTGGGTATTTAGT
CGTCAAAGATATTGGGGTGAACCATTCCCTGTAATTTATGATGAAGATGGACAAATTCAT
TTAGTACCAGATGATGAATTACCACTAGAATTACCTTATCTTGAATTTATAAAACCAAGT
GGAACAGGTGAATCACCACTAGCTAACGCACGTGATTGGTTATATGTAGATATCAACGGT
AAAAAAGGTAGACGTGATACCAACACCATGCCACAACTAGCAGGTTCAAGCTGGTATTAT
ATTGGCTACATCCTAAAATCTAATTTAGGTTATATCCCACTTGACTCTAAGGAAGCTAAA
GCAGTACTTGATCAGTTCTTACCAGTCGATCTTTATGTTGGTGGAACAGAACATGCAGTA
GGTCACTTACTTTATGCAAGATTCTGGCATAAATTCTTCTATGACCTAGGTTTTGTAAGT
TCTAAAGAACCTTTCATGAAACTTGTAAACCAAGGTATGATTTTAGGTGCTGACCACTCT
AAGATGAGTAAATCAAAAGGAAATAGTGTCTCACCCGATGACTTTATTCAAAGTCATGGT
GCAGATGCACTAAGACTATTTGAAATGTTTATGGGACCTTTAGAAGCTGAAAAACCTTGG
AGTAATGAAGGACTAGAGGGTGCAAAACGTTTCTTAGACCGTGTTTGGCGAATGTTTGAT
TTTGAAATCACACAACAACCAGTAGATGCGCTTCAAACGGTTTATCACCAAACAGTCAAA
AAAGTTACAGAAGATTATGAAAAGTTAGCATTTAATACTGCAATCTCACAAATGATGATC
TTTGTTAATGAAGTTTATAAGACTAAAGTAATTGGTGCAGCTCAAGCAAGAGGATTCTTA
AAATTATTAAATCCAATTGCACCACACTTAACAGAAGAAATTAATGAGACTGTGTTAAAA
CATCATGAAGATTTAGTTTATTCTGAATGGCCAACCTATGATGAATCATTCTTAATTGAA
ACTGAAGTTGAAGTCGCTATCCAAGTAAACGGAAAACTACGTGGTAGACTTCAAGTGAAA
AAAGATATGGTCGAAGAAGATCTTAAAAAGTTAGCACTTGAACATGAAAATGTCATCAAG
CATGTTGAAGGATTAACAATTGTTAAAGTTATTGTTATACCAAATAAGATTGTAAATATC
GTCGTAAGATAA

>protein sequence
MHKYEHRKIEEKWQKHWLDNKLFKTKNDRFHQKYYVLDMFPYPSASGLHVGHIEGYTATD
IVSRFKRMQGYNVLHPMGWDAYGLPSEQYALATGKDPKSFTYQNISNFKRQIIEMGKGVD
WDKELATTDPEYFKWTQWIFKRLYEKGLAVLKDVEVNWCEGLGTVLANDEIEIINGQMVS
ERGHYPVIKKPMRQWVLRITEYADRLLDDLALVDWPENLKEMQRNWIGKSSGAVIEFEVA
STDYKFEIFTTRPDTIYGVTYCVLSPEHPLVEKIVTQDEEKDVLRYIEQTKMKSDLDRIA
DKSKTGVFTGAFAKHPLTNQLIPIWIGDYVLPQYGMGAVMAVPAHDDRDYEFAMQYGLNI
IEVIKGGSEGAYTGDGIHHHSGIIDGLYNEEAIEKITKHLEDRKKGYKHYTYKLRDWVFS
RQRYWGEPFPVIYDEDGQIHLVPDDELPLELPYLEFIKPSGTGESPLANARDWLYVDING
KKGRRDTNTMPQLAGSSWYYIGYILKSNLGYIPLDSKEAKAVLDQFLPVDLYVGGTEHAV
GHLLYARFWHKFFYDLGFVSSKEPFMKLVNQGMILGADHSKMSKSKGNSVSPDDFIQSHG
ADALRLFEMFMGPLEAEKPWSNEGLEGAKRFLDRVWRMFDFEITQQPVDALQTVYHQTVK
KVTEDYEKLAFNTAISQMMIFVNEVYKTKVIGAAQARGFLKLLNPIAPHLTEEINETVLK
HHEDLVYSEWPTYDESFLIETEVEVAIQVNGKLRGRLQVKKDMVEEDLKKLALEHENVIK
HVEGLTIVKVIVIPNKIVNIVVR