ACL_RS01225

Uniprot: A9NEU5
Description: phenylalanine--tRNA ligase subunit alpha
EC number: 6.1.1.20
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). {ECO:0000255|HAMAP-Rule:MF_00281}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00281}; ; Note=Binds 2 magnesium ions per tetramer. {ECO:0000255|HAMAP-Rule:MF_00281}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 255 255 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00281}.
Nucleotide binding:
Site:
Gene names (primary): pheS
Gene names (synonym):
Mass: 38,573
Subunit structure [CC]: SUBUNIT: Tetramer of two alpha and two beta subunits. {ECO:0000255|HAMAP-Rule:MF_00281}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; phenylalanine-tRNA ligase activity [GO:0004826]; tRNA binding [GO:0000049]; phenylalanyl-tRNA aminoacylation [GO:0006432]
Gene ontology IDs: GO:0000049; GO:0000287; GO:0004826; GO:0005524; GO:0005737; GO:0006432
Chain: CHAIN 1 337 Phenylalanine--tRNA ligase alpha subunit. /FTId=PRO_1000078824.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_00281}.
Protein families: Class-II aminoacyl-tRNA synthetase family, Phe-tRNA synthetase alpha subunit type 1 subfamily
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEU5
MEROPS:
EnsemblBacteria KO: ABX80875
UniPathway: K01889
CDD:
Gene3D:
HAMAP:
InterPro: MF_00281
PANTHER: IPR006195;IPR004529;IPR004188;IPR022911;IPR002319;IPR010978
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50862
ProDom: PF02912;PF01409
SMART:
SUPFAM:
TIGRFAMs: SSF46589

248034-249048(+)

>nucleotide sequence
ATGAAGGAAAAAATAGATTCGATTGTTAAATCATTAAATGATAAAATCCATCCGGATTTA
ACATTAAATGATTTAGAAGCATTAAGAGTTGAGTATCTAGGAAAAAATGGTATTTTAACA
TCTCAAATGTCTCTAATTAGAACTTTACCAAATGAAGAAAAACCTAAGTTTGGTCAATGG
ATCAATGATGCAAAGGTTGCAATAGAACAAGTAATTAGTGATCAAAAAAACATATTACTT
ACAATAGCAATGAATGAACAATTAAAAAAAGAAACACTTGATGTTACAATGCCAGGGTTA
AACTTTAAAAAAGGTAGCTTACACCCATTAACATTAGTGACTCAAGATTTAGAAGATTAC
TTTATTGGTTTAGGCTATAAAGTTGCTGAAGGTCCAGAACTAGAGTTAGATAAATATAAC
TTTGAAATGATGAATCTAGCAAGTGATCACCCTGCTAGAGAAATGCATGATTCTTTTTAT
GTAACTCAAAATACATTACTAAGAACACATACATCTCCAATTCAAGCACGCTATATGGAA
AAATCAAAGGGGCAACCAATCGCAATTATTGCTCCAGGTAAAACCTATAGAAGAGATCCA
GATGATCGTACGCACTCACACCAATTTATGCAATGTGAAGGTTTAGTCATTGATAAGGAT
GTGACATTTGCACACCTTAAAGAAACGCTACTTGGTATGGCACGTCATATCTTTGGTGAA
GAAAGAACGATTCGTTTAAGACCATCTTACTTCCCATTTACTGAACCATCTGTTGAAGTC
GATGTATCTTATGTGGACAGTGATGGTAAAACAGAGTGGATCGAAGTTTTAGGTGCTGGT
ATGGTACACCCAAATGTGTTAACTATGGGTGGATATGATCCTAAAGTATACCGTGGGTTT
GCTTTTGGGATTGGACTTGAACGTATTGCCATCTTAAAATATAAAATCGATGACATTCGT
CAATTTTATACTAATGATCTACGCTTCTTAAATCAATTTAAAGGAGTCTTATAA

>protein sequence
MKEKIDSIVKSLNDKIHPDLTLNDLEALRVEYLGKNGILTSQMSLIRTLPNEEKPKFGQW
INDAKVAIEQVISDQKNILLTIAMNEQLKKETLDVTMPGLNFKKGSLHPLTLVTQDLEDY
FIGLGYKVAEGPELELDKYNFEMMNLASDHPAREMHDSFYVTQNTLLRTHTSPIQARYME
KSKGQPIAIIAPGKTYRRDPDDRTHSHQFMQCEGLVIDKDVTFAHLKETLLGMARHIFGE
ERTIRLRPSYFPFTEPSVEVDVSYVDSDGKTEWIEVLGAGMVHPNVLTMGGYDPKVYRGF
AFGIGLERIAILKYKIDDIRQFYTNDLRFLNQFKGVL