ACL_RS01230

Uniprot: A9NEU6
Description: phenylalanine--tRNA ligase subunit beta
EC number: 6.1.1.20
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00022241}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00283}; ; Note=Binds 2 magnesium ions per tetramer. {ECO:0000256|HAMAP-Rule:MF_00283}
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 452 452 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 458 458 Magnesium; via carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 461 461 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.; METAL 462 462 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00283}.
Nucleotide binding:
Site:
Gene names (primary): pheT
Gene names (synonym):
Mass: 87,243
Subunit structure [CC]: SUBUNIT: Tetramer of two alpha and two beta subunits. {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00022357}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; phenylalanine-tRNA ligase activity [GO:0004826]; tRNA binding [GO:0000049]; phenylalanyl-tRNA aminoacylation [GO:0006432]; tRNA processing [GO:0008033]
Gene ontology IDs: GO:0000049; GO:0000287; GO:0004826; GO:0005524; GO:0005737; GO:0006432; GO:0008033
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_00283, ECO:0000256|SAAS:SAAS00570605}.; SIMILARITY: Contains 1 B5 domain. {ECO:0000256|HAMAP-Rule:MF_00283}.; SIMILARITY: Contains 1 FDX-ACB domain. {ECO:0000256|HAMAP-Rule:MF_00283}.; SIMILARITY: Contains 1 tRNA-binding domain. {ECO:0000256|HAMAP-Rule:MF_00283}.
Protein families: Phenylalanyl-tRNA synthetase beta subunit family, Type 1 subfamily
Coiled coil:
Domain [FT]: DOMAIN 35 149 TRNA-binding. {ECO:0000259|PROSITE:PS50886}.; DOMAIN 399 474 B5. {ECO:0000259|PROSITE:PS51483}.; DOMAIN 690 783 FDX-ACB. {ECO:0000259|PROSITE:PS51447}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEU6
MEROPS:
EnsemblBacteria KO: ABX80876
UniPathway: K01890
CDD:
Gene3D: cd02796
HAMAP: 2.40.50.140;3.30.56.20;3.30.70.380;3.50.40.10
InterPro: MF_00283
PANTHER: IPR005146;IPR009061;IPR012340;IPR004532;IPR020825;IPR005121;IPR002547;IPR033714;IPR005147
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51483;PS51447;PS50886
ProDom: PF03483;PF03484;PF03147;PF01588
SMART:
SUPFAM: SM00873;SM00874;SM00896
TIGRFAMs: SSF46955;SSF50249;SSF54991;SSF56037

249047-251402(+)

>nucleotide sequence
ATGTTTATATTAGATAATATATTAAAACAATTTATTGAAGTACCAAATGATTTATATGAA
AAGACCAATCAACAAATCATCGAAGTCGATGATTATTTAGCATTAAATTCATCGACTAAA
CTTGTAGTAGGTTATGTTGAGACATGTGTTAAACACCCAAATGCCGATACTTTAAGCAAA
ACTACAGTTAATATTGGTAGTGAAGTTTTAGACATCGTTTGTGGGGCACCAAACGTGCGC
AGCGGGCAATATGTTATTGTTGCTCAAGTAGGTTCTGTATTACCAGGTAATTTTGAAATT
AAAGCAGCAGTCGTTCGTGGTGAAACATCAAACGGTATGATCTGTTCATTAAAAGAATTA
GGATTTGATGAAAAACACGTACCAGCTCAATATAAAGATGGCATCTACAATTTTAGCCAA
AAGGTAACACCTGGTGAGGATGCACTTAAAGCACTTGGTCTTGATGGAATGAAACTACTT
TTAGGATTAACACCTAATAGAGGGGATTTATTATCTCACTTAGGGTTTGCTTATGATTTA
GCTTCAATGACTAATAAAAAGGTACATATTCCAAAACCAACATTTAAAGAAGCAGCACAT
AAACATGATGTTCAAGTCGTACTCGACACAAATAACTGTTTAACATATGATTTAAGAGTT
ATGGATGTTGTGATTAAAGAAAGTCCATGGTGGTTAAAAAACGCACTAATTCAAAGCGAT
ATTCGTCCGATTAATAACGTTGTAGATATTACAAACTACATCTTAATTACTTACGGTACA
CCACTTCATGCATTTGATGCAAACAAAGTGAATTCAAATACAATTTTAGTACGTCAAGCG
CATCAAGGTGAAGAAGTTGTTACATTAGATGAAGTTTCACGTGTATTAGAACCGTCAGAC
ATCGTCATTACAGATGGTAAAAAACCAATCGCACTTGGTGGTGTGATGGGCTTATTAGAA
TCTGGTATTGATGATCATACAACAAAAATCATGCTTGAAGCAGCACAGTTTAATCCAAGT
GTGATTGCTAAAACATCTAAACGTTTAGATTTAAGAAGTGATTCATCACTACGCTTTGAA
CGTGGTATTGATCAAAAAAGAGTACGTATTGGTTTAGAAGCGGCTACTCAACTACTTGTT
GAGTTAGCAGATGCTAAAGTGTATTCAGGTATTTCATCAAAAACAAATGTAAAAGAAGAA
AACCCTTGGGTTGAACTATCCTTAGAAAACGTAAATGGTTTATTAGGTACTAAGTTAAAT
GATAATGAATTACATGATATACTTGCTCAGTTAAACTATGAAACAAAAATAGATAACAAA
ACATTATTAGTTCAAGCACCTGGTTATAGAAAAGATATCTTAATTGAAGCAGATGTTATT
GAAGAAGTCTTACGTGTTTATGGGTATCACCATATTGAAGGTAAGAAACTTCAACCAAAC
TTATTAGGTGAACTATCTTATAGTCAAAAAATGAATAGGGCACTTAAACAAACATTAGTG
GGTTTAGGCTTAAATGAAGTGATTAACTATAGTTTAGTATCAGAATCAGATATTGAGTCA
TTTGCTAAAGTTGGCGAATCAGTAAGTATTTTAATGCCTCTAAGTGATGACCGTAAGATT
TTACGTCAATCCTTAATACCAGGGTTACTAAAGAACTTAAACTATCATTTATCAAGACAA
ATGTCACACATTACACTATTTGAAATGGGTCACGTCTTTGCAAAAGATGTCGAAAAAAAT
CATTTAGCAGTATTAATCAATGGTAATTTACTAGATTCTAACTACTTAAAACGTGATATT
AAATCAGACTTCTATACATTAAAAGGTATCTTAGAACACATTGGTGTGACACTAAATGTC
TCATTTGATGTGAAACAATCTTCTAATGTAGCTGCCCTACACCCTGGTATCCAAGGCGAT
GTTTATGTAGGGGATTTACTTGTTGGGGTCATTGGTAAAACACACCCATTAACTGATGAA
GCTTATGATATTAAAGATGCTTATGTATTAGAATTAGACTTATCTCAACTAATTACAACA
AAAGCACCAATGATTTTTGAAAGTATTTCTAAGTATCCATCCATCCAAAGAGATATCTCA
TTTGTAGTATCTAAAGACTATCCAATTAGTGATATTTTAGGTATTATTAAACAAACAGCT
AGAAAACTATTAACCAACATTGAAGTGTTTGATGTGTATAGTGGATCAAATATTGAAGAA
GGATATCACTCACTAGCAATCTCAATGACATTTAATAATAAAGAAAAAACATTAGAAAAA
TTAGATGTAGAAAAAGCACTCAAATCCATTCAAAACAGATTAGAATTCAATTTTAAAGCA
GTATTTAGAGCATAA

>protein sequence
MFILDNILKQFIEVPNDLYEKTNQQIIEVDDYLALNSSTKLVVGYVETCVKHPNADTLSK
TTVNIGSEVLDIVCGAPNVRSGQYVIVAQVGSVLPGNFEIKAAVVRGETSNGMICSLKEL
GFDEKHVPAQYKDGIYNFSQKVTPGEDALKALGLDGMKLLLGLTPNRGDLLSHLGFAYDL
ASMTNKKVHIPKPTFKEAAHKHDVQVVLDTNNCLTYDLRVMDVVIKESPWWLKNALIQSD
IRPINNVVDITNYILITYGTPLHAFDANKVNSNTILVRQAHQGEEVVTLDEVSRVLEPSD
IVITDGKKPIALGGVMGLLESGIDDHTTKIMLEAAQFNPSVIAKTSKRLDLRSDSSLRFE
RGIDQKRVRIGLEAATQLLVELADAKVYSGISSKTNVKEENPWVELSLENVNGLLGTKLN
DNELHDILAQLNYETKIDNKTLLVQAPGYRKDILIEADVIEEVLRVYGYHHIEGKKLQPN
LLGELSYSQKMNRALKQTLVGLGLNEVINYSLVSESDIESFAKVGESVSILMPLSDDRKI
LRQSLIPGLLKNLNYHLSRQMSHITLFEMGHVFAKDVEKNHLAVLINGNLLDSNYLKRDI
KSDFYTLKGILEHIGVTLNVSFDVKQSSNVAALHPGIQGDVYVGDLLVGVIGKTHPLTDE
AYDIKDAYVLELDLSQLITTKAPMIFESISKYPSIQRDISFVVSKDYPISDILGIIKQTA
RKLLTNIEVFDVYSGSNIEEGYHSLAISMTFNNKEKTLEKLDVEKALKSIQNRLEFNFKA
VFRA