ACL_RS01240

Uniprot: A9NEU8
Description: ribosome small subunit-dependent GTPase
EC number: 3.6.1.-
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_01820}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01820}
Enzyme regulation:
Function [CC]: FUNCTION: May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00535149}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 247 247 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 253 253 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 255 255 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.; METAL 261 261 Zinc. {ECO:0000256|HAMAP-Rule:MF_01820}.
Nucleotide binding: NP_BIND 113 116 GTP. {ECO:0000256|HAMAP-Rule:MF_01820}.; NP_BIND 166 174 GTP. {ECO:0000256|HAMAP-Rule:MF_01820}.
Site:
Gene names (primary): rsgA
Gene names (synonym):
Mass: 34,051
Subunit structure [CC]: SUBUNIT: Monomer. Associates with ribosomes. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00151859}.
Gene ontology (GO): GTP binding [GO:0005525]; GTPase activity [GO:0003924]; metal ion binding [GO:0046872]
Gene ontology IDs: GO:0003924; GO:0005525; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily. {ECO:0000256|HAMAP-Rule:MF_01820, ECO:0000256|SAAS:SAAS00535129}.; SIMILARITY: Contains CP-type G (guanine nucleotide-binding) domain. {ECO:0000256|SAAS:SAAS00064598}.
Protein families: TRAFAC class YlqF/YawG GTPase family, RsgA subfamily
Coiled coil:
Domain [FT]: DOMAIN 64 223 CP-type G (guanine nucleotide-binding). {ECO:0000259|PROSITE:PS51721}.; DOMAIN 73 221 EngC GTPase. {ECO:0000259|PROSITE:PS50936}.
Motif: MOTIF 247 261 Knuckle-like cysteine cluster. {ECO:0000256|HAMAP-Rule:MF_01820}.
Region:
EMBL: CP000896
ProteinModelPortal: A9NEU8
MEROPS:
EnsemblBacteria KO: ABX80878
UniPathway: K06949
CDD:
Gene3D:
HAMAP: 3.40.50.300
InterPro: MF_01820
PANTHER: IPR030378;IPR027417;IPR004881;IPR010914
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50936;PS51721
ProDom: PF03193
SMART:
SUPFAM:
TIGRFAMs: SSF52540

252503-253457(+)

>nucleotide sequence
TTGAATAAAGCACGTGTAATTAAATTAATCGGAGGCTTATATAGCTTTAAAGACCTTAAG
ACGCAAACAGTGTATGAAGGTTATGCAAAGGGTAAATTTAGAAAGGTGAGAGTGGATAAA
GACTCAAGCTTTAATAAGAACATCACAAAAAGCACTAAAAAAGATGTCAAAGACATCGTT
TTAAGTCCTAAAGTTGGTGACATTATCCATTATAGTTTTGAATCTGATCAGTACATGATT
ACTGAAGTGCTTGAAAGAAAAAATGAACTTATTAGACCAGATGTTGCAAACATTGATCAA
GTATTATTGGTCTTTTCGGCCATGAGACCGGATTTTAGTTTTAATTTATTGGATAAATTT
CTAGTGATATTAAAACATCATGAATTAACACCTGTTATTGTGGTATCTAAAGTAGACTTA
ATGACGGATGAAAATTTAAAATCTCTTAAAGAAGATTTAAAATTTTATGAACCATTTTAC
GATATACATTATGTTAATAGTAAAGCACGCATTGGTATTGATACATTAGATGGTATATTT
GAAGATAAAATCACGATTTTAGCAGGTCAAACAGGCGCAGGCAAGTCAACACTTTTAAAT
GCACTTAGACCTGAACTAGAATTAAAAACTCAAGAAATATCAGATGCTCTTGGTCGTGGA
AAGCACACAACCAGACATTCTGAACTCTACGAGTTTGGTGGTGGGTATATTTGTGATACA
CCAGGATTTAGTAAAGTAGACTTTGAATTTCATGATTATACGACACTAAAAGACTACTTT
GTAGACTTTGCTAAGTATAGTGATCAGTGTAAGTTTGGTCATAGTTGTTTACATATCAAC
GAGCCTAATTGCATGGTTAAAGAAAAAATCAAATCAAAAGAAATATTACAATCAAGGTAT
GATAGTTACTTAAATTTCGTAGAAATATTAAAAAATAGAAAAGAAAAGTATTAG

>protein sequence
LNKARVIKLIGGLYSFKDLKTQTVYEGYAKGKFRKVRVDKDSSFNKNITKSTKKDVKDIV
LSPKVGDIIHYSFESDQYMITEVLERKNELIRPDVANIDQVLLVFSAMRPDFSFNLLDKF
LVILKHHELTPVIVVSKVDLMTDENLKSLKEDLKFYEPFYDIHYVNSKARIGIDTLDGIF
EDKITILAGQTGAGKSTLLNALRPELELKTQEISDALGRGKHTTRHSELYEFGGGYICDT
PGFSKVDFEFHDYTTLKDYFVDFAKYSDQCKFGHSCLHINEPNCMVKEKIKSKEILQSRY
DSYLNFVEILKNRKEKY