ACL_RS01395

Uniprot: A9NEX6
Description: N-acetylmuramic acid 6-phosphate etherase
EC number: 4.2.1.126
Annotation score: 3 out of 5
Miscellaneous [CC]: MISCELLANEOUS: A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product. {ECO:0000256|HAMAP-Rule:MF_00068}.
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510857}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00570226}.
Pathway: PATHWAY: Amino-sugar metabolism; N-acetylmuramate degradation. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510845}.
Active site: ACT_SITE 84 84 Proton donor. {ECO:0000256|HAMAP-Rule:MF_00068}.; ACT_SITE 115 115 {ECO:0000256|HAMAP-Rule:MF_00068}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): glmS
Gene names (synonym): murQ
Mass: 32,725
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00510849}.
Gene ontology (GO): carbohydrate binding [GO:0030246]; carbon-oxygen lyase activity [GO:0016835]; transaminase activity [GO:0008483]; carbohydrate metabolic process [GO:0005975]; N-acetylmuramic acid catabolic process [GO:0097173]
Gene ontology IDs: GO:0005975; GO:0008483; GO:0016835; GO:0030246; GO:0097173
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily. {ECO:0000256|HAMAP-Rule:MF_00068, ECO:0000256|SAAS:SAAS00570217}.; SIMILARITY: Contains 1 SIS domain. {ECO:0000256|HAMAP-Rule:MF_00068}.
Protein families: GCKR-like family, MurNAc-6-P etherase subfamily
Coiled coil:
Domain [FT]: DOMAIN 56 219 SIS. {ECO:0000259|PROSITE:PS51464}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEX6
MEROPS:
EnsemblBacteria KO: ABX80906
UniPathway: K07106
CDD:
Gene3D:
HAMAP:
InterPro: MF_00068
PANTHER: IPR005488;IPR005486;IPR001347
PIRSF:
PRINTS:
PROSITE:
Pfam: PS01272;PS51464
ProDom: PF13580
SMART:
SUPFAM:
TIGRFAMs:

294454-295369(+)

>nucleotide sequence
ATGGTAGATATTAAATCCATTACAACTGAACAACGCAATAAATTAACTAAAAATTTAGAT
GTTAGTTCAACAACAGAAATACTAAAAATGTTTAATAATGAGGACAAAAATGTACCTTTA
GCTGTAGAAAAAGTACTTGATAAGATTGCTTTAACAGTAGATGAAGTTACTAAACGACTT
GCAGAAGGCGGTAGACTATTTTACATTGGTGCAGGAACGTCCGGCAGATTAGGTGTTTTA
GATGCCTCTGAATGTGTTCCAACCTTTGGTGTTTCTGAAAACTTGGTCATCGGTATTATT
GCAGGTGGCGATAGAGCATTAAGAATCGCTGTTGAAGGCGCTGAAGATGATGAAGTAGCA
GGTGTTAATGACCTTAAAGCTTATAACTTTAATGAAAAAGACTTTGTTGTAGGTTTAACT
GCAAGTGGTCGTACACCATATGTTATGGCAGCTATGAAATATGCGCGTAGTATTGGTGCT
AAGGCAGCAGGTATTACAACATCTCATAATGCACCATTATCTCAAGTAGTAGATTTTCCA
ATTGAGGCAGTAACAGGTGCTGAACCGTTAACAGGTTCTACACGTATGAAATCAGGAACT
GCTCAAAAACTAATTTTAAATATGATATCCACAGCAACCATGGTAAAACTAGGTAAAGTC
TATGAAAACTTAATGATCGATATTCAAATGAGTAACGATAAATTAGTTTCACGTGCAGTA
GGTATTGTTGTAGAAGTTACAGGTGTTGATCATGATGTTGCAAAAGTTTACATTGATAAA
TTTAAATCTGTAAAACACGCAATATTCGCAATCATCTCAAAATTAGATAATATTGAAGAA
ATTAACGAAGTATTAAATAAATACAATGGTAACATTAGAGAAGCATTGAAACATTCTATA
TCATTTGGAGGATAA

>protein sequence
MVDIKSITTEQRNKLTKNLDVSSTTEILKMFNNEDKNVPLAVEKVLDKIALTVDEVTKRL
AEGGRLFYIGAGTSGRLGVLDASECVPTFGVSENLVIGIIAGGDRALRIAVEGAEDDEVA
GVNDLKAYNFNEKDFVVGLTASGRTPYVMAAMKYARSIGAKAAGITTSHNAPLSQVVDFP
IEAVTGAEPLTGSTRMKSGTAQKLILNMISTATMVKLGKVYENLMIDIQMSNDKLVSRAV
GIVVEVTGVDHDVAKVYIDKFKSVKHAIFAIISKLDNIEEINEVLNKYNGNIREALKHSI
SFGG