ACL_RS01405

Uniprot: A9NEX8
Description: glucosamine-6-phosphate deaminase
EC number: 3.5.99.6
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Alpha-D-glucosamine 6-phosphate + H(2)O = D-fructose 6-phosphate + NH(3). {ECO:0000255|HAMAP-Rule:MF_01241}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion. {ECO:0000255|HAMAP-Rule:MF_01241}.
Pathway: PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5. {ECO:0000255|HAMAP-Rule:MF_01241}.
Active site: ACT_SITE 67 67 Proton acceptor; for enolization step. {ECO:0000255|HAMAP-Rule:MF_01241}.; ACT_SITE 134 134 For ring-opening step. {ECO:0000255|HAMAP-Rule:MF_01241}.; ACT_SITE 136 136 Proton acceptor; for ring-opening step. {ECO:0000255|HAMAP-Rule:MF_01241}.; ACT_SITE 141 141 For ring-opening step. {ECO:0000255|HAMAP-Rule:MF_01241}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): nagB
Gene names (synonym):
Mass: 26,786
Subunit structure [CC]:
Gene ontology (GO): glucosamine-6-phosphate deaminase activity [GO:0004342]; hydrolase activity [GO:0016787]; carbohydrate metabolic process [GO:0005975]; N-acetylglucosamine metabolic process [GO:0006044]; N-acetylneuraminate catabolic process [GO:0019262]
Gene ontology IDs: GO:0004342; GO:0005975; GO:0006044; GO:0016787; GO:0019262
Chain: CHAIN 1 240 Glucosamine-6-phosphate deaminase. /FTId=PRO_1000085745.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily. {ECO:0000255|HAMAP-Rule:MF_01241}.
Protein families: Glucosamine/galactosamine-6-phosphate isomerase family, NagB subfamily
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NEX8
MEROPS:
EnsemblBacteria KO: ABX80908
UniPathway: K02564
CDD:
Gene3D:
HAMAP:
InterPro: MF_01241
PANTHER: IPR006148;IPR004547
PIRSF: PTHR11280
PRINTS:
PROSITE:
Pfam:
ProDom: PF01182
SMART:
SUPFAM:
TIGRFAMs:

295842-296565(+)

>nucleotide sequence
TTGAAAATAAACGTATTTGAGACAAAAGAAGAATTATATAGAGCAGTAGCTGATTTTTAT
ATTAAGGCAATCAATGAAAAACCAAATATGACATTAGGTTTAGCAACAGGTACAACACCG
ATTCCTTTATACCAAAATCTGATCAAAGCATATCAAGATAAATTAGTATCATTCAAAGAT
ATTACAACATTTAACTTAGATGAATATATTGGGTTACCTAAAACACATAAAGAATCATAC
TTTAGTTTTATGAGAAATCAATTATTTAATCATGTAGATATTAATTTAGATAATACACAT
ATTCCAAGTGGTGTATTAGAGCCTAGTGAAGCAATAAAAGAGTTTCAAACTGCTTTAGAT
GCTCATCAGATAGACATCCAATTATTAGGCTTAGGATCTAACGGACATATCGGTTTTAAT
GAACCAGGTACATCATTTGAATCTACAACGCATAAAACACAACTTGCATTATCAACTATT
CAAGATAACTCAAGAATGTTTGATTCTATTGATGAGGTTCCAACTGAATCAATTACAATG
GGTATTAAAGATATTATGCGTGCATCTAAAATCGTTATGATAGCAACAGGTGCTCAAAAA
GCTGATGCTGTTTACAAAATGATTCAAGGTCCAGTAGATGAAAGTCTACCAGCATCAATT
TTACAAAAACATGATGATGTAGTTATCTTCCTAGATAAAGACGCAGCATCGTTATTAAAA
TAG

>protein sequence
LKINVFETKEELYRAVADFYIKAINEKPNMTLGLATGTTPIPLYQNLIKAYQDKLVSFKD
ITTFNLDEYIGLPKTHKESYFSFMRNQLFNHVDINLDNTHIPSGVLEPSEAIKEFQTALD
AHQIDIQLLGLGSNGHIGFNEPGTSFESTTHKTQLALSTIQDNSRMFDSIDEVPTESITM
GIKDIMRASKIVMIATGAQKADAVYKMIQGPVDESLPASILQKHDDVVIFLDKDAASLLK