ACL_RS01640

Uniprot: A9NF20
Description: S-adenosylmethionine synthase
EC number: 2.5.1.6
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine. {ECO:0000255|HAMAP-Rule:MF_00086}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00086}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000255|HAMAP-Rule:MF_00086}; ; Note=Binds 2 divalent ions per subunit. Magnesium or cobalt. {ECO:0000255|HAMAP-Rule:MF_00086};; COFACTOR: Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000255|HAMAP-Rule:MF_00086}; ; Note=Binds 1 potassium ion per subunit. {ECO:0000255|HAMAP-Rule:MF_00086}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. {ECO:0000255|HAMAP-Rule:MF_00086}.
Pathway: PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00086}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 16 16 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00086}.; METAL 42 42 Potassium. {ECO:0000255|HAMAP-Rule:MF_00086}.; METAL 267 267 Potassium. {ECO:0000255|HAMAP-Rule:MF_00086}.; METAL 275 275 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00086}.
Nucleotide binding: NP_BIND 263 270 ATP. {ECO:0000255|HAMAP-Rule:MF_00086}.
Site:
Gene names (primary): metK
Gene names (synonym):
Mass: 42,288
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00086}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; methionine adenosyltransferase activity [GO:0004478]; one-carbon metabolic process [GO:0006730]; S-adenosylmethionine biosynthetic process [GO:0006556]
Gene ontology IDs: GO:0000287; GO:0004478; GO:0005524; GO:0005737; GO:0006556; GO:0006730
Chain: CHAIN 1 386 S-adenosylmethionine synthase. /FTId=PRO_1000075365.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the AdoMet synthase family. {ECO:0000255|HAMAP-Rule:MF_00086}.
Protein families: AdoMet synthase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF20
MEROPS:
EnsemblBacteria KO: ABX80950
UniPathway: K00789
CDD:
Gene3D:
HAMAP:
InterPro: MF_00086
PANTHER: IPR022631;IPR022630;IPR022629;IPR022628;IPR002133;IPR022636
PIRSF: PTHR11964
PRINTS: PIRSF000497
PROSITE:
Pfam: PS00376;PS00377
ProDom: PF02773;PF02772;PF00438
SMART:
SUPFAM:
TIGRFAMs: SSF55973

338496-339657(+)

>nucleotide sequence
ATGAGTTATATTTTTTCAAGCGAAAGTGTGTCTAAAGGACATCCAGATAAAGTGGCGGAT
TTTATAGCAGATTCAGTATTAGATTTAGTATTAAAAAATGACCCTAAAGCACGTTGTGCG
ATTGAGGTTGCTTTAGCACACACAACTGTTTATATTTTTGGTGAGGTATCAACAAGTTAT
ATATTAACAGATGAAATCATTATTGAAACTGCAAAAAAAGCAATCGATTTTTGTGGTTAT
AATGATGAGAAATTCATCTTTGATGCAAAATCAGCAACCTATCATGTAAACATTCATAAA
CAAAGTGCAGATATCGCGCTAGGGGTTGATGAAACAGATAATAAAGAACAAGGTGCAGGG
GATCAAGGTATTATGTTTGGTTATGCTAAAGATGATACGAAAGAACTTTTACCTTTACCG
ATTTTTCTAGCACATGAATTAACTAAGCGTTTAGCTTTTGTTAGAGAAGAAGGTATTGTA
TCCGGACTTGGACCAGATGGTAAATCTCAAGTATCTGTTTTATATAATGAAAACCATGAA
CCACTGGAAGTTACAGCGATTGTATTATCTACACAACATGAAGCATCCAAAACACTTGAA
AGTGTTAAAGCAGACATGATGAAACATGTTATCTTAGAAGTCATTCCAAAACATTTAATT
ACAGATAACACTAAATACTATATTAATCCAACCGGTAGATTCGTCATTGGTGGACCAGTA
GGAGATAGTGGCTTAACAGGTCGTAAACTTATTGTAGACACTTACGGTGGATACTCACGC
CACGGTGGTGGTGCATTTAGTGGTAAAGATGCATCTAAAGTAGACAGATCAGCATCTTAT
ATGGCAAGATACTTAGCAAAACAAGTCGTAGGAAGTGGTATTGCAAAAACTTGTGAAATC
CAATTAGCTTATGCTATTGGTGTTGCAGAACCTGTAAGTTTATATGTAAACACTTTTGGT
ACATCTAAAGTAAGTAATGAGGTAATTGCTGAAACAATCTCCAAACACTTTGATTTAAGA
CCTAAAGCAATTATTAATTATCTTGGACTTACGAACCCAATTTTCAAACAAACCACACAT
AATGGCCATTTTGGTAAGTTAGAATCTAATTTAAGTTGGGAAAAATTAGATAAAATTGAA
ATTTTCGAACAATTAGTATAA

>protein sequence
MSYIFSSESVSKGHPDKVADFIADSVLDLVLKNDPKARCAIEVALAHTTVYIFGEVSTSY
ILTDEIIIETAKKAIDFCGYNDEKFIFDAKSATYHVNIHKQSADIALGVDETDNKEQGAG
DQGIMFGYAKDDTKELLPLPIFLAHELTKRLAFVREEGIVSGLGPDGKSQVSVLYNENHE
PLEVTAIVLSTQHEASKTLESVKADMMKHVILEVIPKHLITDNTKYYINPTGRFVIGGPV
GDSGLTGRKLIVDTYGGYSRHGGGAFSGKDASKVDRSASYMARYLAKQVVGSGIAKTCEI
QLAYAIGVAEPVSLYVNTFGTSKVSNEVIAETISKHFDLRPKAIINYLGLTNPIFKQTTH
NGHFGKLESNLSWEKLDKIEIFEQLV