ACL_RS01750

Uniprot: A9NF42
Description: DNA polymerase I
EC number: 2.7.7.7
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). {ECO:0000256|RuleBase:RU004460}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): polA
Gene names (synonym):
Mass: 100,085
Subunit structure [CC]:
Gene ontology (GO): 3'-5' exonuclease activity [GO:0008408]; DNA binding [GO:0003677]; DNA-directed DNA polymerase activity [GO:0003887]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0003677; GO:0003887; GO:0006261; GO:0008408
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DNA polymerase type-A family. {ECO:0000256|RuleBase:RU004459}.
Protein families: DNA polymerase type-A family
Coiled coil: COILED 498 525 {ECO:0000256|SAM:Coils}.
Domain [FT]: DOMAIN 2 264 53EXOc. {ECO:0000259|SMART:SM00475}.; DOMAIN 306 474 3'-5' exonuclease. {ECO:0000259|SMART:SM00474}.; DOMAIN 640 844 POLAc. {ECO:0000259|SMART:SM00482}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF42
MEROPS:
EnsemblBacteria KO: ABX80972
UniPathway: K02335
CDD:
Gene3D:
HAMAP: 3.30.420.10;3.40.50.1010
InterPro:
PANTHER: IPR002562;IPR020046;IPR020045;IPR002421;IPR019760;IPR001098;IPR018320;IPR002298;IPR008918;IPR029060;IPR012337
PIRSF:
PRINTS:
PROSITE: PR00868
Pfam: PS00447
ProDom: PF01367;PF02739;PF00476
SMART:
SUPFAM: SM00474;SM00475;SM00279;SM00482
TIGRFAMs: SSF47807;SSF53098;SSF88723

365756-368399(+)

>nucleotide sequence
ATGAAAAAAATTACACTTGTAGACGGTAACTCGATACTTTTTAGAGCATATAATGCGACT
GCATATCCAGGAGCTAAGGTCTTACAAACATCCACAGGTATTTATACAAATGCCGTTTTT
GCATTTAGTGGTATGATCGATAAAATTATTGAGATATCTAATTCACATATGCTTATAGCA
TTTGATACTGGAGAACCGACACACAGACATAAATCATATGATGATTATAAAGCAGGTAGA
AAAGAAATGCCTGAGGAATTAGGTAGTCAAATACCTTTAATCCATGAACTTATTAAGTAT
CTTGGCATTAAAGAATACTCCATGGCTGGTTATGAAGCAGATGACATCATTGGTACCCTT
GCTAAAATGGCTGAAAAAGCTGGTTATAAAGTCGATGTCTTTTCATCTGATTATGACCTT
TTACAATTAGTTACAGAACATATTACTGTGCACATGTTAAAAAAAGGTATGCGTGTTGTA
GAAACCTACACACCTGAAACTTTAAAAGAAACTTATGGTTTTTCTCATGAGTTATTTATT
GATTTTAAAGCATTAGTTGGTGATAAATCTGATAATATTCCAGGTGTTCCAGGCGTTGGA
GAAGTCACTGCTAAAAAGTTAATTGCTGAATATGGTACGTTAGAAAATATTTTAAATAAT
GCAGAACATATTAAAGGTAAGCTTGGAGAAAACATTCGTACAAACAAAGAACAGGCAGTT
TTTTCAAAAGAACTCTCAACAATTCTAACGGATATGCCACTACCTTTTGATCTTGAAGAT
ACAAAAGTTGGTGAAGTAGATGAAGAAAGCTTAATTAATTTTTATAAACGTCTTGAATTA
AAACAATTAGTGATATCTTATAATAAGAAAAATCCAGAAGTTGCTGCAACTACTGCCGAA
ACTTCTGAGTTTCAATATAAAGCAATTGATTCTGAAAATGCATTAAAATCTCTACTCAAC
CAAGATCTTGCAATTTACTTTGAATTTTCAGATTTCAATTACCACAAAGCTGAACTTTGG
GGTGTTGGTTTAAGTAATGGAAAAATCAATTACTTTGTAGATAGTGAAACGTTTTTAAAT
AGTGCCTCATTTAAAGCTTATTTAGAAAGTTCTAAATATAAAAAGTATACATATCACTAT
AAAGGTACAAAAGTATTTTTAAAGAAACTTGGTACCGAGTTTAATCATGTAACCTATGAT
TTACTTTTAGCTGCTTACTTAATTCAGTCCAGTATTGGTAAACAAGACTTTACCTTTATT
GCGCAACAATTTCAAATCACGGATATTCAATATGATGAAGAAGTTTATGGTAAAGGTGCT
AAAAAGGCCCTACCTTTAATTCCATCAGACTATGAAGGTCATATAGCTAAAAAGGCGTAT
ATCATCCATAAACTGATGCAACAAACACTAGATGTATTAACTGAACGTAACCAATTAAGT
TTACTAAATGATGTGGAACTACCTCTATCAGATGTCCTTGCAGATATGGAATATCAAGGT
TTAACAGTTGACCTAAAAGAACTAGAAAATCAAACTAAAGATATGTCTTTACGTATTGAT
AAATTAAGAACTGAAATTCTTTTGCTAGCAGGTGTTGACTTTAATGTCGATAGTCCTAAA
CAATTAGGTGATGTCTTATTTGATACCTTAGGTCTACCAAATGGTAAGAAAACTAAAACA
GGATACTCTACAGGTATTGAAGTTTTAAACAACCTAATCGATCACCATCCAATTATTAAC
TTAATTATTGAATATAGACAACTAACTAAACTGTATTCAACCTATTTAATCGGTATTAAA
GAATCCGTATTTGAAGATAACCGTGTACACACCATTTATAATCAAGCACTAACCTTAACT
GGTCGCTTATCAAGTTTAGAACCGAACTTACAAAATATTCCTATTCGTACAGAAGAAGGT
AGACAAATAAGAAAATTATTTATTCCTGAACCTGATAGCTATTTTGTAGGTGCTGACTAC
TCTCAAATCGAATTACGTGTGTTAGCAGCTATGGCTGATGTTAAAAATTTAAAACAAGCA
TTTGAAGAAGATCAAGACATTCATACCGTAACAGCTCAAAAGGTATTCCACGTAGATACT
GTCGATAGTGAACAAAGAAGACGTGCTAAAGCAGTTAACTTCGGTATTATTTATGGTATT
GGACCATGGAGTTTATCTGAGGATATTGGTGTCACGGTGAAAGAAGCAGAAAACTTTATT
AGCCGTTATTTGGAAGTATATCCTGAAATTAAAGATTACATGACCAATATCGTTGAGTTT
GCTAAAACACATGGCTATGTTGAAACACTTTTAAATCGTCGCCGTTATATACCAGAACTA
TCCTCTAAAGTATTTAACTTAAGAGAGTTTGGTAAACGTACCAGTTTAAATGCGCCGATT
CAAGGTACAGCAGCAGACATTATTAAACTCGCGATGATTGATTTACACAACTATTTAAAA
GATAATAAAAAGAAATCAAAACTTATCCTACAAGTACATGACGAACTCATCGTTGAGGTT
GTTAAAGAAGAGTTGGATGAGATGAAAGAAGTCATACCAAACATCATGGAAAAAGCCTTT
AATTTAGGCGTTAGACTGAAAACATCATGTGATGTTGGAAACAACTGGTATGAATTAAAA
TAA

>protein sequence
MKKITLVDGNSILFRAYNATAYPGAKVLQTSTGIYTNAVFAFSGMIDKIIEISNSHMLIA
FDTGEPTHRHKSYDDYKAGRKEMPEELGSQIPLIHELIKYLGIKEYSMAGYEADDIIGTL
AKMAEKAGYKVDVFSSDYDLLQLVTEHITVHMLKKGMRVVETYTPETLKETYGFSHELFI
DFKALVGDKSDNIPGVPGVGEVTAKKLIAEYGTLENILNNAEHIKGKLGENIRTNKEQAV
FSKELSTILTDMPLPFDLEDTKVGEVDEESLINFYKRLELKQLVISYNKKNPEVAATTAE
TSEFQYKAIDSENALKSLLNQDLAIYFEFSDFNYHKAELWGVGLSNGKINYFVDSETFLN
SASFKAYLESSKYKKYTYHYKGTKVFLKKLGTEFNHVTYDLLLAAYLIQSSIGKQDFTFI
AQQFQITDIQYDEEVYGKGAKKALPLIPSDYEGHIAKKAYIIHKLMQQTLDVLTERNQLS
LLNDVELPLSDVLADMEYQGLTVDLKELENQTKDMSLRIDKLRTEILLLAGVDFNVDSPK
QLGDVLFDTLGLPNGKKTKTGYSTGIEVLNNLIDHHPIINLIIEYRQLTKLYSTYLIGIK
ESVFEDNRVHTIYNQALTLTGRLSSLEPNLQNIPIRTEEGRQIRKLFIPEPDSYFVGADY
SQIELRVLAAMADVKNLKQAFEEDQDIHTVTAQKVFHVDTVDSEQRRRAKAVNFGIIYGI
GPWSLSEDIGVTVKEAENFISRYLEVYPEIKDYMTNIVEFAKTHGYVETLLNRRRYIPEL
SSKVFNLREFGKRTSLNAPIQGTAADIIKLAMIDLHNYLKDNKKKSKLILQVHDELIVEV
VKEELDEMKEVIPNIMEKAFNLGVRLKTSCDVGNNWYELK