ACL_RS01825

Uniprot: A9NF57
Description: GTPase ObgE
EC number: 3.6.5.-
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01454};
Enzyme regulation:
Function [CC]: FUNCTION: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. {ECO:0000255|HAMAP-Rule:MF_01454}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 173 173 Magnesium. {ECO:0000255|HAMAP-Rule:MF_01454}.; METAL 193 193 Magnesium. {ECO:0000255|HAMAP-Rule:MF_01454}.
Nucleotide binding: NP_BIND 166 173 GTP. {ECO:0000255|HAMAP-Rule:MF_01454}.; NP_BIND 191 195 GTP. {ECO:0000255|HAMAP-Rule:MF_01454}.; NP_BIND 212 215 GTP. {ECO:0000255|HAMAP-Rule:MF_01454}.; NP_BIND 279 282 GTP. {ECO:0000255|HAMAP-Rule:MF_01454}.; NP_BIND 306 308 GTP. {ECO:0000255|HAMAP-Rule:MF_01454}.
Site:
Gene names (primary): obg
Gene names (synonym):
Mass: 45,872
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01454}.
Gene ontology (GO): cytoplasm [GO:0005737]; GTP binding [GO:0005525]; GTPase activity [GO:0003924]; magnesium ion binding [GO:0000287]; ribosome biogenesis [GO:0042254]
Gene ontology IDs: GO:0000287; GO:0003924; GO:0005525; GO:0005737; GO:0042254
Chain: CHAIN 1 419 GTPase Obg. /FTId=PRO_0000385656.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. {ECO:0000255|HAMAP-Rule:MF_01454}.; SIMILARITY: Contains 1 OBG-type G (guanine nucleotide-binding) domain. {ECO:0000255|HAMAP-Rule:MF_01454}.
Protein families: TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family
Coiled coil:
Domain [FT]: DOMAIN 160 325 OBG-type G. {ECO:0000255|HAMAP-Rule:MF_01454}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF57
MEROPS:
EnsemblBacteria KO: ABX80987
UniPathway: K03979
CDD:
Gene3D:
HAMAP: 2.70.210.12;3.40.50.300
InterPro: MF_01454
PANTHER: IPR031167;IPR014100;IPR015349;IPR006169;IPR006073;IPR027417;IPR005225
PIRSF: PTHR11702:SF3
PRINTS:
PROSITE: PR00326
Pfam: PS51710
ProDom: PF09269;PF01018;PF01926
SMART:
SUPFAM:
TIGRFAMs: SSF102741;SSF52540;SSF82051

382163-383423(+)

>nucleotide sequence
ATGAGCGCATTTGTAGATGCAGTAACAGTAGAAGTAAAAGCAGGACGCGGTGGTAACGGT
AAAGTTGCATTCCGTAGAGAAGCACACGTGGAGTTTGGTGGACCCGCTGGTGGTAATGGT
GGCCGTGGTGGTCATATTTACTTTATTGGTGATGAAGGTAAAAATACGTTAATAGATTTA
AAATACAACAGACATATCAAAGCTGCTAATGGTGTCCATGGTGGCCCTAAAGGGATGCAT
GGCGCACACGCAGAAGATACATATGTTCGTGTACCATTAGGTACAATTGTATATGATGAT
AAAGAAAATTTAATTGGTGAAGTGTTAGAACATGGTCAAACTTTATTAATTGCTCAAGGT
GGCAAAGGTGGCCGTGGTAATATGGCGTTTGCATCCAACAATAATAAAGCACCTGACTTT
GCAGAACAAGGTGATTTAGGTCAAATATTCTTAGCAAAAGTAGAACTTCAAGTCCTAGCA
GATGTTGGTTTATTAGGTTATCCTAATGTTGGTAAATCGACTTTAATTACTAGAATCTCA
AATGCAAAAGCTAAAATAGCTGACTACCAATTTACAACACTATCTCCACAACTTGGTATG
GTTAATGTGGAAGATGATGCGTTTGTTGTTGCAGACTTACCTGGATTAATTGAGTTTGCG
CACCTTGGTGTTGGTTTAGGCTTACAATTCTTAAAACACGTTGAACGTTGTAGAGTTTTA
TTACATATCGTATCTATGGATTCATTAGATCCACTAGATGACTACAATAAGATTAACAAT
GAATTAGTGTTATATGATGAAAAACTAAAAGACAGAACTCAAATAGTCGTAGCAAATAAG
ATGGACGTTGAAGGTGCACAAGAAAAGTTTGAAGCCTTCAAAAAAGCATTAAAAGATGTT
AAAGTTATTCCGATTTCAGCACTGATGAATGATGGTATTCAAACGCTTAAATATGAAATT
GCTACAACATTAAAAACTATCCCTAAATTTGCTCCAAAAGATACTACAAAACACTATACA
CTGAATGCAGAAGATGCAGTAGATTTCATCATCAATAAAGGTGATGACGGTGTCTTTGAA
TTAACAGGTGATAAATTATTTGTTTTATTTAATAGAACAGACTTTAATAATGAATCAGCT
GTGAAACGTTTTGCAAGACAGCTACGTGGTTTAGGTATCGAAGATGCACTTAGAGAACAT
GGTGTTGTTCATGGTGATATCGTACGTATCTTCTCTTATGAATTTGAATATTTAGAATAA

>protein sequence
MSAFVDAVTVEVKAGRGGNGKVAFRREAHVEFGGPAGGNGGRGGHIYFIGDEGKNTLIDL
KYNRHIKAANGVHGGPKGMHGAHAEDTYVRVPLGTIVYDDKENLIGEVLEHGQTLLIAQG
GKGGRGNMAFASNNNKAPDFAEQGDLGQIFLAKVELQVLADVGLLGYPNVGKSTLITRIS
NAKAKIADYQFTTLSPQLGMVNVEDDAFVVADLPGLIEFAHLGVGLGLQFLKHVERCRVL
LHIVSMDSLDPLDDYNKINNELVLYDEKLKDRTQIVVANKMDVEGAQEKFEAFKKALKDV
KVIPISALMNDGIQTLKYEIATTLKTIPKFAPKDTTKHYTLNAEDAVDFIINKGDDGVFE
LTGDKLFVLFNRTDFNNESAVKRFARQLRGLGIEDALREHGVVHGDIVRIFSYEFEYLE