ACL_RS01845

Uniprot: A9NF61
Description: Holliday junction ATP-dependent DNA helicase RuvA
EC number: 3.6.4.12
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. {ECO:0000255|HAMAP-Rule:MF_00031}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. {ECO:0000255|HAMAP-Rule:MF_00031}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): ruvA
Gene names (synonym):
Mass: 20,723
Subunit structure [CC]: SUBUNIT: Forms a complex with RuvB. {ECO:0000255|HAMAP-Rule:MF_00031}.
Gene ontology (GO): Holliday junction helicase complex [GO:0009379]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; four-way junction helicase activity [GO:0009378]; DNA recombination [GO:0006310]; DNA repair [GO:0006281]; SOS response [GO:0009432]
Gene ontology IDs: GO:0003677; GO:0005524; GO:0006281; GO:0006310; GO:0009378; GO:0009379; GO:0009432
Chain: CHAIN 1 186 Holliday junction ATP-dependent DNA helicase RuvA. /FTId=PRO_1000074408.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RuvA family. {ECO:0000255|HAMAP-Rule:MF_00031}.
Protein families: RuvA family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF61
MEROPS:
EnsemblBacteria KO: ABX80991
UniPathway: K03550
CDD:
Gene3D: cd14332
HAMAP: 2.40.50.140
InterPro: MF_00031
PANTHER: IPR013849;IPR003583;IPR012340;IPR000085;IPR010994;IPR011114
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF07499;PF01330
SMART:
SUPFAM: SM00278
TIGRFAMs: SSF46929;SSF47781;SSF50249

388989-389550(+)

>nucleotide sequence
ATGTATCGATACATTAAAGGTATAGTAACACAAATTAATCCACAACATATTGTTGTTGAA
AATAATGGTGTGGGCTATTTAGTCTTAAGTCCAGTACCATATCAATATAAAATTGGTGAA
GAAACAACAGTAGTAACTTATTTACATGTAAGAGAGGATATTTTTCAACTTTATGGATTT
AAAGATGAAGAAACTTTAAATCTATTTTTAAAACTTATAAGTGTATCTGGTATTGGTCCA
AAATCTGCAATGAGTATTGTAGCATTTGATGATACAAATAAAATTATTGCAGCCATTGAA
ACATCAGATGCAAAATATTTAACTAAGTTTCCTGGTATTGGGATGAAGAGTGCTCAACAA
ATTATTTTAGACTTAAAAGGTAAACTGGTAAATGATGAACTAGATATGCAACTTTTAAGT
GATAACTCTAAGGATGTAGCTGCTGCATTAGAAGCATTAGGCTATAATAAAAAAGAGATT
GCAAAATCATTAAAGCATGTTAATTTTGATCAAGACTTAAATAAAGCACTAAAAGAAGCA
CTTGCAATATTATTAAAGTAG

>protein sequence
MYRYIKGIVTQINPQHIVVENNGVGYLVLSPVPYQYKIGEETTVVTYLHVREDIFQLYGF
KDEETLNLFLKLISVSGIGPKSAMSIVAFDDTNKIIAAIETSDAKYLTKFPGIGMKSAQQ
IILDLKGKLVNDELDMQLLSDNSKDVAAALEALGYNKKEIAKSLKHVNFDQDLNKALKEA
LAILLK