ACL_RS01850

Uniprot: A9NF62
Description: Holliday junction DNA helicase RuvB
EC number: 3.6.4.12
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. {ECO:0000255|HAMAP-Rule:MF_00016}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. {ECO:0000255|HAMAP-Rule:MF_00016}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 60 67 ATP. {ECO:0000255|HAMAP-Rule:MF_00016}.
Site:
Gene names (primary): ruvB
Gene names (synonym):
Mass: 38,143
Subunit structure [CC]: SUBUNIT: Forms a complex with RuvA. {ECO:0000255|HAMAP-Rule:MF_00016}.
Gene ontology (GO): ATP binding [GO:0005524]; DNA binding [GO:0003677]; four-way junction helicase activity [GO:0009378]; DNA recombination [GO:0006310]; DNA repair [GO:0006281]; SOS response [GO:0009432]
Gene ontology IDs: GO:0003677; GO:0005524; GO:0006281; GO:0006310; GO:0009378; GO:0009432
Chain: CHAIN 1 337 Holliday junction ATP-dependent DNA helicase RuvB. /FTId=PRO_1000074070.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the RuvB family. {ECO:0000255|HAMAP-Rule:MF_00016}.
Protein families: RuvB family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF62
MEROPS:
EnsemblBacteria KO: ABX80992
UniPathway: K03551
CDD:
Gene3D:
HAMAP: 1.10.10.10;3.40.50.300
InterPro: MF_00016
PANTHER: IPR003593;IPR004605;IPR008823;IPR027417;IPR008824;IPR011991
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF05491;PF05496
SMART: PD005323
SUPFAM: SM00382
TIGRFAMs: SSF46785;SSF52540

389559-390573(+)

>nucleotide sequence
ATGAGTGAAGAAAAATCAGTATTAAGAGATTTAAATTTAAAAAATGATGATGAATTGATG
CTTCGTCCTCAAACCTTAAACCAATATATAGGGCAAGATGATATTAAAGAAATGTTATCT
ATTTATATTCAGGCAGCATTAAAACGTGAAGAAAGTTTAGATCACGTTTTACTTTATGGT
GCACCGGGTTTAGGTAAGACAACACTTGCTCAAATTATCGCTAATGAGTTAGGTGTAGAT
ATCAAAATAACAAGTGGTCCAGCTATAGAAAAAACAGGTGACTTAGTAGCCCTTCTTTCT
TCATTAAGTCCAGGAGATGTGTTATTTATTGATGAAATTCACCGTATACCTCGTTTTGTT
GAAGAGGTTCTTTATTCTGCAATGGAAGATTATACGCTTGATATTGTTCTAGATAAGGAA
AGAGATTCTAGAAGTATTAGAATCGAATTACCACCGTTTACACTCATTGGTGCAACCACT
AGATTTGGTGATTTATCACACCCGTTAAGGGAACGATTTGGTGCTGTATTTAGATTAAGT
TATTACAAGTTAGAAGAAATCAAACAAATTGTAAGAAGAACTTCTAAAGTATATCAAAAT
GAAATTGATGAAAAAGCAGTAGATGAATTATCTAAGCGTAGTAGAGGTACACCTAGAATA
GCAAACCGTCTATTTAGACGCGTAAGAGACTTTGCTGAAATTATGACAGATGCCGTGATA
ACACTAGATATTACACAGTTAGCATTGACCAAATTAGGCATTGATCATAAGGGTCTGGAT
GCATCAGACTATTTATATTTAAGAGGCATTGTAGAGCGATTTAATGGTGGTCCTGTTGGA
CTTGAATCACTGGCTTCTACAATTGGTGAAGAACCAGGAACAATTGAAGATGTATATGAA
CCTTACTTGTTACAGGAAGGTTATATTAAACGTACACCAAGAGGTAGAGTTGCAACAGAA
CTTGCATACAACCTTTTAGGTGTGAAATATTATAAAGGATTATTAGACAATTGA

>protein sequence
MSEEKSVLRDLNLKNDDELMLRPQTLNQYIGQDDIKEMLSIYIQAALKREESLDHVLLYG
APGLGKTTLAQIIANELGVDIKITSGPAIEKTGDLVALLSSLSPGDVLFIDEIHRIPRFV
EEVLYSAMEDYTLDIVLDKERDSRSIRIELPPFTLIGATTRFGDLSHPLRERFGAVFRLS
YYKLEEIKQIVRRTSKVYQNEIDEKAVDELSKRSRGTPRIANRLFRRVRDFAEIMTDAVI
TLDITQLALTKLGIDHKGLDASDYLYLRGIVERFNGGPVGLESLASTIGEEPGTIEDVYE
PYLLQEGYIKRTPRGRVATELAYNLLGVKYYKGLLDNW