ACL_RS01855

Uniprot: A9NF63
Description: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
EC number: 2.4.99.17
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA. {ECO:0000256|SAAS:SAAS00094485}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA). {ECO:0000256|SAAS:SAAS00094494}.
Pathway: PATHWAY: tRNA modification; tRNA-queuosine biosynthesis. {ECO:0000256|SAAS:SAAS00094444}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): queA
Gene names (synonym):
Mass: 31,928
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|SAAS:SAAS00094423}.
Gene ontology (GO): cytoplasm [GO:0005737]; isomerase activity [GO:0016853]; transferase activity [GO:0016740]; queuosine biosynthetic process [GO:0008616]
Gene ontology IDs: GO:0005737; GO:0008616; GO:0016740; GO:0016853
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the QueA family. {ECO:0000256|SAAS:SAAS00573562}.
Protein families: QueA family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF63
MEROPS:
EnsemblBacteria KO: ABX80993
UniPathway: K07568
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR003699
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF02547
SMART:
SUPFAM:
TIGRFAMs: SSF111337

390569-391589(+)

>nucleotide sequence
TTGAAATTAGAATTATTTGATTATGATTTACCCAAATCACATATAGCGCAACATCCAAAA
GATAAACGAGACCACTCAAAATTATTGGTTGTTGATAGAGAAAAACAAACAACAAAAGAT
TGTTTGTTTTATGACATCTTAGATGAGTTAAGTAGCAATGATGTACTAGTTATGAATAAC
ACAAAAGTTATCCCTGCAAGATTAATTGGTGAAAAGATCGATACGCATGCAGTCATTGAA
GTTTTACTACTTAAAGAGTTAGAAAAAAACACTTGGGAAGCTTTAACGAAACCTGCCAAA
AAAATTAAAATTGGTACTGTAGTAAGTTTTGGTGATGGTGCACTTAAAATGGAGTGTATT
GGCATTAAAGATGAAGGTATTCGCATCTTTAAAGCAATCTATGAAGGTATATTTTTAGAA
GTACTAGATGCTTTAGGTACCATGCCACTACCACCTTACATAACGGAAAAATTAGAAGAT
AAATCAAGATATCAAACTGTTTATGCAAAATATGCAGGATCTGCTGCAGCACCTACTGCA
GGACTCCATTTTACCAAAGAACTTTTAGAAAAGATTAAAGCCAAAGGTGTTCAAGTATTA
GAAATTACACTCCATGTTGGTTTAGGTACATTTAGACCAGTTCAAGTAGATGATGTATTA
GAACATCAAATGCACTATGAAAGTTATGAGATAAACGAAGAGACTGCCCGATTATTAAAC
CAATATAAAAAAGAAGGTAAAAGAATTATCGCAGTTGGTACAACCACTGTAAGAACACTA
GAATCTAACTATAATGATGGATTCCATGCAGGTAATTACTCAACCAACATCTTTATTTAT
CCGGGATATCAATTTAAGATCATTGGTGCGATTATCACTAATTTCCATTTACCAAAATCA
ACGCTCATCATGTTGATTTCAGCATTCTATAATAGAGAAAAAGTGCTTGAAGTCTATGAA
GACGCAGTGAAAAAAAACTACCGCTTTTTCTCATTTGGCGATAGTATGTTTATTAAATAA

>protein sequence
LKLELFDYDLPKSHIAQHPKDKRDHSKLLVVDREKQTTKDCLFYDILDELSSNDVLVMNN
TKVIPARLIGEKIDTHAVIEVLLLKELEKNTWEALTKPAKKIKIGTVVSFGDGALKMECI
GIKDEGIRIFKAIYEGIFLEVLDALGTMPLPPYITEKLEDKSRYQTVYAKYAGSAAAPTA
GLHFTKELLEKIKAKGVQVLEITLHVGLGTFRPVQVDDVLEHQMHYESYEINEETARLLN
QYKKEGKRIIAVGTTTVRTLESNYNDGFHAGNYSTNIFIYPGYQFKIIGAIITNFHLPKS
TLIMLISAFYNREKVLEVYEDAVKKNYRFFSFGDSMFIK