ACL_RS01880

Uniprot: A9NF68
Description: methyltransferase
EC number: 2.1.1.33
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_01057}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_01057}.
Pathway: PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. {ECO:0000256|HAMAP-Rule:MF_01057}.
Active site:
Binding site: BINDING 37 37 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_01057}.; BINDING 62 62 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_01057}.; BINDING 89 89 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_01057}.; BINDING 111 111 S-adenosyl-L-methionine. {ECO:0000256|HAMAP-Rule:MF_01057}.; BINDING 115 115 Substrate. {ECO:0000256|HAMAP-Rule:MF_01057}.; BINDING 147 147 Substrate. {ECO:0000256|HAMAP-Rule:MF_01057}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): trmB
Gene names (synonym):
Mass: 23,471
Subunit structure [CC]:
Gene ontology (GO): tRNA (guanine-N7-)-methyltransferase activity [GO:0008176]
Gene ontology IDs: GO:0008176
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family. {ECO:0000256|HAMAP-Rule:MF_01057}.
Protein families: Class I-like SAM-binding methyltransferase superfamily, TrmB family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 180 183 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_01057}.
EMBL: CP000896
ProteinModelPortal: A9NF68
MEROPS:
EnsemblBacteria KO: ABX80998
UniPathway: K03439
CDD:
Gene3D:
HAMAP: 3.40.50.150
InterPro: MF_01057
PANTHER: IPR029063;IPR003358
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51625
ProDom: PF02390
SMART:
SUPFAM:
TIGRFAMs: SSF53335

394034-394643(+)

>nucleotide sequence
ATGAGACAAAAGAAAATTAAAGATGCAACGATTGAAAATCTAGAAAAGTTAGGTGTTTTA
TTAGCGCCTACACCACTTAAAGTAGATGCTTCAAAAAAGATCACATTAGAAATTGGTAGT
GGTAAAGGGCGTTTTATCACATCACTTGCTAATGATTTCCCAGAAGAAGTATTTATTGCA
GTAGAACGTGATCAAAATGTGTGTTACCGTTTAGCGCAAAAAAAAAGTGTTTTAGAGTTG
AAAAACTTGATTGTTATTATGGATGATGCAGAAAAATTAAATGAATATTTAGTGGATGTA
AAAGTAGATAACCTACATTTAAATTTCTCTGACCCATGGCCAAAGAAAAAGCACCATAAA
CGTAGGTTGACTTATCCTACGATGTTAAATCAATATAAAAACTACTTAAAAAAAGATGGA
AAAATCATCTTTAGAACAGATCATGCAGATTTATTCGTTGACTCATTTCAATACTTTAAC
GAAGCATCGTTCATTGTATCAGATTGTAACTGGGCACTTGCACCATCAGATTATATGACA
GAATATGAAGTTAAGAAACGTGTAAGTGGTCCAATATATCAATTAATAGCGGAGCAAAAT
CATGACTAA

>protein sequence
MRQKKIKDATIENLEKLGVLLAPTPLKVDASKKITLEIGSGKGRFITSLANDFPEEVFIA
VERDQNVCYRLAQKKSVLELKNLIVIMDDAEKLNEYLVDVKVDNLHLNFSDPWPKKKHHK
RRLTYPTMLNQYKNYLKKDGKIIFRTDHADLFVDSFQYFNEASFIVSDCNWALAPSDYMT
EYEVKKRVSGPIYQLIAEQNHD