ACL_RS01900

Uniprot: A9NF72
Description: DNA gyrase subunit A
EC number: 5.99.1.3
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP-dependent breakage, passage and rejoining of double-stranded DNA. {ECO:0000256|HAMAP-Rule:MF_00937, ECO:0000256|SAAS:SAAS00075911}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. {ECO:0000256|HAMAP-Rule:MF_00937}.
Pathway:
Active site: ACT_SITE 130 130 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000256|HAMAP-Rule:MF_00937}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site: SITE 50 50 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 86 86 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 88 88 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 99 99 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 105 105 Interaction with DNA. {ECO:0000256|HAMAP-Rule:MF_00937}.; SITE 129 129 Transition state stabilizer. {ECO:0000256|HAMAP-Rule:MF_00937}.
Gene names (primary): parC
Gene names (synonym):
Mass: 97,766
Subunit structure [CC]: SUBUNIT: Heterotetramer composed of ParC and ParE. {ECO:0000256|HAMAP-Rule:MF_00937}.
Gene ontology (GO): chromosome [GO:0005694]; extrinsic component of plasma membrane [GO:0019897]; ATP binding [GO:0005524]; DNA binding [GO:0003677]; DNA topoisomerase type II (ATP-hydrolyzing) activity [GO:0003918]; chromosome segregation [GO:0007059]; DNA topological change [GO:0006265]
Gene ontology IDs: GO:0003677; GO:0003918; GO:0005524; GO:0005694; GO:0006265; GO:0007059; GO:0019897
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily. {ECO:0000256|HAMAP-Rule:MF_00937}.
Protein families: Type II topoisomerase GyrA/ParC subunit family, ParC type 2 subfamily
Coiled coil:
Domain [FT]: DOMAIN 19 472 TOP4c. {ECO:0000259|SMART:SM00434}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF72
MEROPS:
EnsemblBacteria KO: ABX81002
UniPathway: K02621
CDD:
Gene3D:
HAMAP: 3.30.1360.40;3.90.199.10
InterPro: MF_00937
PANTHER: IPR024946;IPR006691;IPR013760;IPR002205;IPR013758;IPR005741
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF03989;PF00521
SMART:
SUPFAM: SM00434
TIGRFAMs: SSF56719

398620-401002(+)

>nucleotide sequence
ATAGAAGAAAAAATTGAAAAAGTTCTCTTAGAAGATATTATGTCTTCTAGATTTGGACGA
TACTCTAAATATATCATTCAAGATCGTGCACTACCAGATGCTAGAGATGGTTTAAAACCA
GTTCAACGTCGTATTTTATACGCAATGGGACAAATGGGTTTAGCCTTTAACAAATCATAT
AAAAAATCTGCAAGAATTGCAGGAGAAGTTATGGGGAAATATCACCCTCATGGGGATTCA
TCTATTTATGAAGCAATGGTTCGTATGAGCCAGGACTTTAAAATGAGAGTACCTCTAGTT
GATATGCATGGTAACAATGGTTCCATAGATGGTGACTCTGCTGCGGCAATGCGTTATACC
GAGGCTAGACTATCTAAAGAAGCAGAATTTTTACTTAAAGATATCGATAAAAGAACAGTA
AACTTTGTACCTAACTTTGATGATGAGGAACAAGAACCTACCGTATTACCCGCACGTTTT
CCAAACTTACTTGTCAATGGTGCAATGGGTATTTCAGCAGGTTATGCAACAAAAATACCA
CCACATAACTTAAATGAAGTCATTGATGCAACGATTGCTTTAATTGATAATCCAAATATT
ACAAATGATGAAATATTAAAGTATATAAAAGGACCGGACTTCCCAACAGGTGGTATTGTT
CAAGGTAAAGCAGGTATTAAAGAAGCACTCACCACAGGTAGTGGGAAAATTATTATCAGG
GCTAAGATGAATGTCGAAGAGATGAGTAAATCTCAAGACCGTATTGTGATTACTGAAATA
CCTTATGAAGTTAATAAAGCAGATTTAGTGCGTCAAATTGATAATTTACGTATCAATAAC
AATATCGAGGATATCTTAGAAATCCGTGATGAAACGGACCAAGAAGGTCTAAGAATTGCC
ATTGATCTTAAAAAAGGTGCAGATGCACAATTTATTCAAAACTACTTATTAAAATCAAGT
GATCTTCAAGTAAACTACAACTATAATATGGTTGCTATTTTAAAACACCGTCCAGTTTTA
TCTGGTGCTTTTGAATTATTAAAAGTGTATTTAGAACACCAAAAAGAAGTAGTAACCAAT
AGATCTAATTATGAATTAGAACGTGCAACAAAAAGAAATCATATTGTTGATGGTTTAATT
CAAATGACTTCTGTTGTATCAGAAGTTATTGAAGAAATTAGAAAATCTAAAAATAAAGCA
GACTCAAAAGAAAATATCCAAAAGCGTTTTGGTTTTTCAGAACTACAAGCTGAAGCGATT
GTCATGCTTCAACTATATAGATTATCTACAACCGATATCCAAGCACTACTAGATGAAAAA
GATACTTTATATAAAGAAATTACTAGACTAGAACAAATATTATCGAATGAACGTATTTTA
CTTAAGACCATCAAATCTGAACTTCAAGAAGTTCAAACACTACTTCCCTCACCTAGACGT
ACCGAACTAGAAGCGGAAATTGAAACGATAAAGATTGATGAAAAAGATCTCATCTCAGAT
GAACAAACCTATGTAGCTATCACTAAAGATGGTTACGTAAAACGTGCCTCACTAAGATCT
GTTGTAGCATCTAAAGAAGTAGGTTTAAAAGAAAATGATGCAATGCTTTATGAAGAAGAA
TTAAACACACTAGATACCTTACTCTTATTTACAAATTTAGGTCAATATATTTATTTACCG
GTATATAAAATAGAAGAACAAAAATGGCGTGATCTCGGTGTTTATATTAATAACATTGTA
CCTACCTCAAAAGGTGAAACTATTATACGTGTTTTAAAGGTCAGTGATTTTTCAATTGAT
CAAAAAATATTGCTTACAACAAAACAAGGACAAATGAAACAAGTAAATCTAGCAGATTTT
GAAGTTTCAAGGTATAATAGACCGTTACGCGCAATGAAGTTGTCAAAAGACGATGAATTA
GTATCTGTTGACTATAACCAACTAGACAATATAGTAGCTATTTCTAAACATGGTTATGTC
TTAAGATTCGATACAGAAGAACTGCCAGTATATGGTTTAACCGCTGGTGGGGTTAAATCT
ATGGCATTAGCAGAAGGTGATGAAGTTGCAGTAGCATTTTACGCTAAAGAATCAGATGAT
TTTTACTTCTTAACATCACGCGGGCATATCATTAAAGATACAGTATTAGAATTACCTAAA
TACGCTAGAAATAGACGTGGTATTATTTTAGTAGATCGTATTAAAACATCTCCTCATTTC
ATAATCTCAGCATCTAGAGTATCCAAAACACAAATCAAAGAAGATGTAAAGGTACGTATT
TTATCCACTAAAGAAGCGCTGATTACTACAGTATCTGACTTAAAATATGTTGCGAATAAA
TATGGAAAGAAAATGGTAGAGGACGGTTACTATTTAGAAATA

>protein sequence
IEEKIEKVLLEDIMSSRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMGQMGLAFNKSY
KKSARIAGEVMGKYHPHGDSSIYEAMVRMSQDFKMRVPLVDMHGNNGSIDGDSAAAMRYT
EARLSKEAEFLLKDIDKRTVNFVPNFDDEEQEPTVLPARFPNLLVNGAMGISAGYATKIP
PHNLNEVIDATIALIDNPNITNDEILKYIKGPDFPTGGIVQGKAGIKEALTTGSGKIIIR
AKMNVEEMSKSQDRIVITEIPYEVNKADLVRQIDNLRINNNIEDILEIRDETDQEGLRIA
IDLKKGADAQFIQNYLLKSSDLQVNYNYNMVAILKHRPVLSGAFELLKVYLEHQKEVVTN
RSNYELERATKRNHIVDGLIQMTSVVSEVIEEIRKSKNKADSKENIQKRFGFSELQAEAI
VMLQLYRLSTTDIQALLDEKDTLYKEITRLEQILSNERILLKTIKSELQEVQTLLPSPRR
TELEAEIETIKIDEKDLISDEQTYVAITKDGYVKRASLRSVVASKEVGLKENDAMLYEEE
LNTLDTLLLFTNLGQYIYLPVYKIEEQKWRDLGVYINNIVPTSKGETIIRVLKVSDFSID
QKILLTTKQGQMKQVNLADFEVSRYNRPLRAMKLSKDDELVSVDYNQLDNIVAISKHGYV
LRFDTEELPVYGLTAGGVKSMALAEGDEVAVAFYAKESDDFYFLTSRGHIIKDTVLELPK
YARNRRGIILVDRIKTSPHFIISASRVSKTQIKEDVKVRILSTKEALITTVSDLKYVANK
YGKKMVEDGYYLEI