ACL_RS01975

Uniprot: A9NF86
Description: glycine dehydrogenase (aminomethyl-transferring)
EC number: 1.4.4.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO(2). {ECO:0000256|HAMAP-Rule:MF_00713, ECO:0000256|SAAS:SAAS00022799}.
Cofactor: COFACTOR: Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000256|HAMAP-Rule:MF_00713, ECO:0000256|SAAS:SAAS00167334};
Enzyme regulation:
Function [CC]: FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. {ECO:0000256|HAMAP-Rule:MF_00713}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): gcvPB
Gene names (synonym):
Mass: 53,570
Subunit structure [CC]: SUBUNIT: The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits. {ECO:0000256|HAMAP-Rule:MF_00713, ECO:0000256|SAAS:SAAS00022772}.
Gene ontology (GO): glycine dehydrogenase (decarboxylating) activity [GO:0004375]; glycine decarboxylation via glycine cleavage system [GO:0019464]
Gene ontology IDs: GO:0004375; GO:0019464
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GcvP family. C-terminal subunit subfamily. {ECO:0000256|HAMAP-Rule:MF_00713}.
Protein families: GcvP family, C-terminal subunit subfamily
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NF86
MEROPS:
EnsemblBacteria KO: ABX81016
UniPathway: K00283
CDD:
Gene3D:
HAMAP: 3.40.640.10
InterPro: MF_00713
PANTHER: IPR023012;IPR020581;IPR015424;IPR015421
PIRSF: PTHR11773
PRINTS:
PROSITE:
Pfam:
ProDom: PF02347
SMART:
SUPFAM:
TIGRFAMs: SSF53383

414422-415880(-)

>nucleotide sequence
TTATATGTGAGATAAATCTTTAAATTTTACAAGTGGATTTCTTGCAGCTTTTGCTTCATC
AAGGCGTCTAACCACAGTATGGTGTGGCGCATTTTTAATGAATTCAGGTTTTGTAATCGC
ATCATTTGCAATCGTTTTCATCGTATCAATGAAGTGATCTAGCGTTTCTAAACTTTCCAC
TTCAGTAGGTTCAATCATGAGTGATTGACTAAATGTAAGTGGGAAATATATGGTTGGAGC
ATGTATACCATAATCAAGAAGGCGCTTTGCAACATCTAGTGTTTTAATGTCAGTTGACTG
ATCAATTAAGCCGTCAAAGACAAATTCATGCATTGCGTGTGCCTTAATAGGTAATTTAAA
TAAGTGTTTTAAGCTTTCTTTAATATAGTTTGCATTTAACGTAGCAAGTGGACCAATTTT
ACCTAGATATTCTTCACCAAGCGTTCTAATATATACATATGCTCTTAAATAAACAGAAGC
ATTTCCGTAAAAGGCTGATAAAGCACCGATTGTTTCTTTAGGTGTTTCAAAATGATATGT
ACTACCTTCTTTATAGACGACAGGACCTGGTAAATAATCTTTTAAGAAGTCTTTAACACC
AACTGGACCTGAACCTGGTCCACCACCACCGTGTGGTGTAGAAAAGGTTTTATGTAAGTT
TAAGTGTGTAATATCAAAGCCCATATCACCAGGACGAGCAATACCTAATAAAGCATTAAG
GTTTGCACCATCATAGTAGAGTAATCCACCACAATCATGGATAAGGTCTGCAACTTCTAA
AATATCTTTTTCAAACATACCTACTGTGTTAGGATTTGTTAACATTAAACCAGCAACGGT
ATCATCTAAAGCTTCCTTTAAGGATTCAACATTAATTGTACCATCTAAATTAGATTTGAC
TTCAACAACATCAAATCCAGCAACCGTAGCAGAAGCAGGGTTTGTACCATGTGCAGAATC
AGGAACAATGATTTTATTACGTTTTAAGTCACCTCTATCATGGTGATACTTCTTGATAAT
CATTAAGCCTGCAAGTTCACCTTGTGCACCCGCATAAGTATTTAATGAATAAGCATCCAT
ACCAGTGATTTTGGATAAGTAACTACCAAGTTCAAAGTAAACTTGTAAGAAACCTTGGGA
CACATGATTTGGTTGGAGTGGGTGTATTTGTGAAAACCCAGGTAAAGTAGCAATTTCATC
GTTTATTTTAGGATTATACTTCATCGTACATGAACCTAAAGGATAAAACCCAGTTTCAAC
ACCAAAGTTTTTAAGAGAGACATTGGTATAGTGTCTTACGACATCAAGCTCTGTAACTTC
TGGTAACTCGGCTGCGTCACTTCTTAATAACTCTTTTGGTAATTTAACTTGAGGAAAACT
ATCTTTTGGTAAGTTATGGCCAATACGTCCAGGTTTGGAGATTTCAAATATTAATTTGTC
ATAGTATTTTGCTTTCAT

>protein sequence
MKAKYYDKLIFEISKPGRIGHNLPKDSFPQVKLPKELLRSDAAELPEVTELDVVRHYTNV
SLKNFGVETGFYPLGSCTMKYNPKINDEIATLPGFSQIHPLQPNHVSQGFLQVYFELGSY
LSKITGMDAYSLNTYAGAQGELAGLMIIKKYHHDRGDLKRNKIIVPDSAHGTNPASATVA
GFDVVEVKSNLDGTINVESLKEALDDTVAGLMLTNPNTVGMFEKDILEVADLIHDCGGLL
YYDGANLNALLGIARPGDMGFDITHLNLHKTFSTPHGGGGPGSGPVGVKDFLKDYLPGPV
VYKEGSTYHFETPKETIGALSAFYGNASVYLRAYVYIRTLGEEYLGKIGPLATLNANYIK
ESLKHLFKLPIKAHAMHEFVFDGLIDQSTDIKTLDVAKRLLDYGIHAPTIYFPLTFSQSL
MIEPTEVESLETLDHFIDTMKTIANDAITKPEFIKNAPHHTVVRRLDEAKAARNPLVKFK
DLSHI