ACL_RS02185

Uniprot: A9NFC7
Description: phosphoenolpyruvate--protein phosphotransferase
EC number: 2.7.3.9
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine. {ECO:0000256|PIRNR:PIRNR000732}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|PIRNR:PIRNR000732, ECO:0000256|PIRSR:PIRSR000732-3};
Enzyme regulation:
Function [CC]: FUNCTION: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). {ECO:0000256|PIRNR:PIRNR000732}.
Pathway:
Active site: ACT_SITE 188 188 Tele-phosphohistidine intermediate. {ECO:0000256|PIRSR:PIRSR000732-1}.; ACT_SITE 498 498 Proton donor. {ECO:0000256|PIRSR:PIRSR000732-1}.
Binding site: BINDING 293 293 Substrate. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 329 329 Substrate. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 427 427 Substrate. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 448 448 Substrate; via carbonyl oxygen. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 449 449 Substrate; via amide nitrogen. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 450 450 Substrate. {ECO:0000256|PIRSR:PIRSR000732-2}.; BINDING 451 451 Substrate; via amide nitrogen. {ECO:0000256|PIRSR:PIRSR000732-2}.
Calcium binding:
DNA binding:
Metal binding: METAL 427 427 Magnesium. {ECO:0000256|PIRSR:PIRSR000732-3}.; METAL 451 451 Magnesium. {ECO:0000256|PIRSR:PIRSR000732-3}.
Nucleotide binding:
Site:
Gene names (primary): ptsA
Gene names (synonym):
Mass: 64,185
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; kinase activity [GO:0016301]; metal ion binding [GO:0046872]; phosphoenolpyruvate-protein phosphotransferase activity [GO:0008965]; phosphoenolpyruvate-dependent sugar phosphotransferase system [GO:0009401]
Gene ontology IDs: GO:0005737; GO:0008965; GO:0009401; GO:0016301; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the PEP-utilizing enzyme family. {ECO:0000256|PIRNR:PIRNR000732, ECO:0000256|SAAS:SAAS00563041}.
Protein families: PEP-utilizing enzyme family
Coiled coil:
Domain [FT]: DOMAIN 5 126 PEP-utilisers_N. {ECO:0000259|Pfam:PF05524}.; DOMAIN 151 222 PEP-utilizers. {ECO:0000259|Pfam:PF00391}.; DOMAIN 251 536 PEP-utilizers_C. {ECO:0000259|Pfam:PF02896}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFC7
MEROPS:
EnsemblBacteria KO: ABX81057
UniPathway: K08483
CDD:
Gene3D:
HAMAP: 1.10.274.10;3.20.20.60;3.50.30.10
InterPro:
PANTHER: IPR008279;IPR000121;IPR024692;IPR006318;IPR008731;IPR015813
PIRSF:
PRINTS: PIRSF000732
PROSITE:
Pfam:
ProDom: PF05524;PF00391;PF02896
SMART:
SUPFAM:
TIGRFAMs: SSF47831;SSF51621;SSF52009

458064-459777(+)

>nucleotide sequence
ATGACAAAACTTAAAGGTAATGGTGTATCCAGTGGTATAGCAATTGCTAAGGTACATCAA
TTCAAACTAAATAACATGTTTGCAATGAATAAGTATGCAACCGACAAAGCATTTGAAATC
AAGCGCTTAGGGGATGCACTTTATAAAGCAGATAAGGAACTTGAAAATCTAACCAAGCAA
GCTGAAGAAAGAATTGGGGTTGAACATGCCCAAATCTTTAGTGCGCAACAAATGATGATT
AATGACCCTGAAATGATAAAAAATGCGCATGTTAAAATAAATGACTTTAATCTAGATGCT
GCCTACGCATTTAAGCAAACTATGAGTGAAATGATAGATATGTTTGAGTTAAGTGATAAT
CCATACATTAAGGAACGTATCTCTGATTTAGAAGATATTTCAAATAGAGTATTACATATT
TTAAGTGGAAAAACGCATAGTCTTTATGTGTTTAATGAAGATGTGATACTTGTAGCAACA
GACTTAGTCCCTTCAGAAACAATCAATTTAGACACAACTTTTATTAAAGGTATTGTCATT
GAAAAAGGATCTAAAACAAGTCACTCAGCAATTCTTGCACGTAATTTAGGTATTCCTGCA
ATTACGGGTGTTGATGTAAGTAAAATACATCACGACACACTTGCAATCATTGATGGACAC
ACAGGGGATATTTTATTAGAACCTAGTGAAGATACCATCAAAACATACCAAACTAAACAG
CAAGAAGCACTTAAATTACAATCAACGTATGAAACAATAAAAGACCTTAAAACAGCAACC
ATAGACAATCATGAAGTCCATTTAGCTGCAAACATTGGTGTTGATTTAGACATTCAAGAA
GCACTAGATGCTAATGCTTCAGGTATTGGTTTATTACGTACAGAAAACCAGTATTTAGAA
TCTGATGATTTTCCAACCGAAGATGAACTATTTATCTTCTATGAAAAGGTAGCAACATCA
TTTGAAACTAATGAAGTCACTATTAGAACGCTTGATATTGGTGGCGATAAAAACTTAAGT
TACCTCAATATGCGTAAAGAATTAAACCCATTTTTAGGTAATAGAGCAATCAGATATAGC
CTAAGTTATCCATCATTATTTAAAACTCAGCTTCGTGCTATCTTACGTGCTAATACACAT
CAAAATATTAAAGTCATGTTTCCAATGATTTCAACTTTAAAAGAACTCATGGATGCGAAG
AAGATACTAGATGAAACTTATCAAGCATTAAAGATTGAAGGACATGAAGTAAATTATCCA
AAGATTGGTATGATGGTTGAAGTACCATCCGCTGCACTTGGTATAGAAAAGTTTTTACCA
CATATTGATTTCATCAGTATTGGAACCAATGACCTGATTCAATACATGTTTGCAGCAGAT
AGAATGAATGAACGTGTAGCATATCTTTATCAACCATATAATCCAATTCTTTTGTCCACC
ATCAAATCTATGATTGACAAGACTAATAAGCATGGTATAATAGTTAGCGTGTGCGGTGAA
ATGGCTGGCCATAAAGCTCAAAGTTTACTACTTATTGGCTTAGGTATCAAACATTTAAGC
ATGCATGCAAACTTAATTTTAGAAAACAAATACTTGATTTCTAAAACAACTTATAAGGCA
CTAGAAGAAATTGCCGAAAAAGCATTATTACTAGATCACCCTTACGAAGTTGAAGCACTT
ATAAATACATATATTAAAGGACTTAAATATTAA

>protein sequence
MTKLKGNGVSSGIAIAKVHQFKLNNMFAMNKYATDKAFEIKRLGDALYKADKELENLTKQ
AEERIGVEHAQIFSAQQMMINDPEMIKNAHVKINDFNLDAAYAFKQTMSEMIDMFELSDN
PYIKERISDLEDISNRVLHILSGKTHSLYVFNEDVILVATDLVPSETINLDTTFIKGIVI
EKGSKTSHSAILARNLGIPAITGVDVSKIHHDTLAIIDGHTGDILLEPSEDTIKTYQTKQ
QEALKLQSTYETIKDLKTATIDNHEVHLAANIGVDLDIQEALDANASGIGLLRTENQYLE
SDDFPTEDELFIFYEKVATSFETNEVTIRTLDIGGDKNLSYLNMRKELNPFLGNRAIRYS
LSYPSLFKTQLRAILRANTHQNIKVMFPMISTLKELMDAKKILDETYQALKIEGHEVNYP
KIGMMVEVPSAALGIEKFLPHIDFISIGTNDLIQYMFAADRMNERVAYLYQPYNPILLST
IKSMIDKTNKHGIIVSVCGEMAGHKAQSLLLIGLGIKHLSMHANLILENKYLISKTTYKA
LEEIAEKALLLDHPYEVEALINTYIKGLKY