ACL_RS02305

Uniprot: A9NFF0
Description: 3-oxoacyl-ACP synthase III
EC number: 2.3.1.180
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2). {ECO:0000255|HAMAP-Rule:MF_01815}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids. {ECO:0000255|HAMAP-Rule:MF_01815}.
Pathway: PATHWAY: Lipid metabolism; fatty acid biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01815}.
Active site: ACT_SITE 116 116 {ECO:0000255|HAMAP-Rule:MF_01815}.; ACT_SITE 239 239 {ECO:0000255|HAMAP-Rule:MF_01815}.; ACT_SITE 269 269 {ECO:0000255|HAMAP-Rule:MF_01815}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): fabH
Gene names (synonym):
Mass: 34,122
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01815}.
Gene ontology (GO): cytoplasm [GO:0005737]; 3-oxoacyl-[acyl-carrier-protein] synthase activity [GO:0004315]; beta-ketoacyl-acyl-carrier-protein synthase III activity [GO:0033818]; fatty acid biosynthetic process [GO:0006633]
Gene ontology IDs: GO:0004315; GO:0005737; GO:0006633; GO:0033818
Chain: CHAIN 1 310 3-oxoacyl-[acyl-carrier-protein] synthase 3. /FTId=PRO_1000088304.
Signal peptide:
Domain [CC]: DOMAIN: The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH. {ECO:0000255|HAMAP-Rule:MF_01815}.
Sequence similarities: SIMILARITY: Belongs to the FabH family. {ECO:0000255|HAMAP-Rule:MF_01815}.
Protein families: FabH family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 240 244 ACP-binding. {ECO:0000255|HAMAP-Rule:MF_01815}.
EMBL: CP000896
ProteinModelPortal: A9NFF0
MEROPS:
EnsemblBacteria KO: ABX81080
UniPathway: K00648
CDD:
Gene3D:
HAMAP: 3.40.47.10
InterPro: MF_01815
PANTHER: IPR013751;IPR013747;IPR004655;IPR016039
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF08545;PF08541
SMART:
SUPFAM:
TIGRFAMs: SSF53901

486588-487521(+)

>nucleotide sequence
ATGAAGAACTTGCCGATTAAAATAGTAAGCACTGGTCGTTATGCACCAGATAATATTATG
ACTAATGATGATTTTAGTAAAATATTAGATACGAATGATGAATGGATAACTACTAGAACA
GGTATTAAAAGACGCCATATTGCTCAAGGTGAAACCGCAATTGATATGGCATATAAAGCA
GCTTTAAAGGCTGTTGAAAATAAAAATTATGATAAAGAAAAAATTGATTTAATTATTGTT
GCAAGTATTACATCACCAGTTAAAACACCATCTATTGCAAATTTAATCCAAGCTAAACTT
GGACTAAATCATAAAAATATTGTAGCATTTGATATTAATGCTGCATGTAGTGGGTTTGTT
TATGCGGTTGAAATTGCAGCAAGTATGATTTCATCAGGTAATTATAAATCAGCATTAGTT
ATTGGTTCTGAACATATGTCATCTATCCTTGATTGGGAAGACCGTTCAACTGCTATCCTA
TTTGGTGATGCTGCTGGTGCAGCAATTATTGAAGTAAGTGATAATCCAAAAGATAATGCA
TATTTCTGGAATGGTTCTAGAGGTGATGATACGGGTATTTTATGGATTGATCCAAAAGTA
AAGATGGCTGGACGTGAAGTCTATAAATTTGCAGTGGATATTATGCCTAAAGCTATTCAT
AAAGTACTTGAAAAAGCAGGACTTACAATTGATGATATCGATTATATTATTCCTCATCAA
GCAAACTACAGAATTATTCAAAGTGTTGCTAAAGATATGAATTTACCGATTGAAAGATTT
TTAATGAACCTAGAAGAGTATGGTAATACATCCGCAGCAAGTATTCCATTCTTATTAGAT
GAACATAAGACTAATAACCCTGAAGTTAAAAGAGTTATATTAGTTGGATTTGGCGGCGGA
TTTACTTGGGGTGCAGCCATCCTAAATGTGTAG

>protein sequence
MKNLPIKIVSTGRYAPDNIMTNDDFSKILDTNDEWITTRTGIKRRHIAQGETAIDMAYKA
ALKAVENKNYDKEKIDLIIVASITSPVKTPSIANLIQAKLGLNHKNIVAFDINAACSGFV
YAVEIAASMISSGNYKSALVIGSEHMSSILDWEDRSTAILFGDAAGAAIIEVSDNPKDNA
YFWNGSRGDDTGILWIDPKVKMAGREVYKFAVDIMPKAIHKVLEKAGLTIDDIDYIIPHQ
ANYRIIQSVAKDMNLPIERFLMNLEEYGNTSAASIPFLLDEHKTNNPEVKRVILVGFGGG
FTWGAAILNV