ACL_RS02405

Uniprot: A9NFG9
Description: DNA polymerase IV
EC number: 2.7.7.7
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). {ECO:0000256|HAMAP-Rule:MF_01113, ECO:0000256|SAAS:SAAS00498106}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01113}; ; Note=Binds 2 magnesium ions per subunit. {ECO:0000256|HAMAP-Rule:MF_01113}
Enzyme regulation:
Function [CC]: FUNCTION: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII. {ECO:0000256|HAMAP-Rule:MF_01113}.
Pathway:
Active site: ACT_SITE 123 123 {ECO:0000256|HAMAP-Rule:MF_01113}.
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 26 26 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01113}.; METAL 122 122 Magnesium. {ECO:0000256|HAMAP-Rule:MF_01113}.
Nucleotide binding:
Site: SITE 31 31 Substrate discrimination. {ECO:0000256|HAMAP-Rule:MF_01113}.
Gene names (primary): mucB
Gene names (synonym): dinB
Mass: 43,833
Subunit structure [CC]: SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01113}.
Gene ontology (GO): cytoplasm [GO:0005737]; damaged DNA binding [GO:0003684]; DNA-directed DNA polymerase activity [GO:0003887]; magnesium ion binding [GO:0000287]; DNA repair [GO:0006281]; DNA-dependent DNA replication [GO:0006261]
Gene ontology IDs: GO:0000287; GO:0003684; GO:0003887; GO:0005737; GO:0006261; GO:0006281
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DNA polymerase type-Y family. {ECO:0000256|HAMAP-Rule:MF_01113}.; SIMILARITY: Contains 1 umuC domain. {ECO:0000256|HAMAP-Rule:MF_01113}.
Protein families: DNA polymerase type-Y family
Coiled coil:
Domain [FT]: DOMAIN 22 203 UmuC. {ECO:0000259|PROSITE:PS50173}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFG9
MEROPS:
EnsemblBacteria KO: ABX81099
UniPathway: K02346
CDD:
Gene3D:
HAMAP: 3.30.1490.100
InterPro: MF_01113
PANTHER: IPR017961;IPR022880;IPR001126
PIRSF:
PRINTS:
PROSITE:
Pfam: PS50173
ProDom: PF00817;PF11799
SMART:
SUPFAM:
TIGRFAMs: SSF100879

503831-504941(+)

>nucleotide sequence
TTGAAAAAATCATATAAAGTTATATTTCATATTGATTTAAACGCTTTCTACGCTACATGT
GCCATGATTAAAGAGCCACATTTGAAGCATAGGGTTTTTGTTGTTGGTGGACAATCCTCT
CAACCAAGAGGTGTTGTATCAACAGCAAGCTATAAAGCTAGAAAATATGGTATACATGCT
GGCATGAGCATGTTAGACGCTTTAAATAAATTTCCCAGACTACTTATAGTACCTGTCGAC
TTTAAATTTTACAGAGAAATGTCTAACAAATTTATGGAAGTTTTGGATGCTTATTCAGAT
TTAGTCCTTCAAGCATCCATTGATGAAGCGTTTTTAGATATGACCAAAGCATCTGAGGAG
ATACACCCAATTAAGTTGGCTAAAAAGATTCAAAAGGAACTGGTTGATAAATACCAGTTA
CCAACATCTATTGGTATCGCACCAACATTATTTTTAGCCAAAATGGCAAGTGATATGAAA
AAACCATTAGGTATTACGGTGTTAAGAAAAAGAGATGTAGAAAAGTTACTATATCCTTTA
AGTGTTACTGACATTTATGGTATCGGTAAACAAACATACCCAAAACTCATGGCACTTGGT
ATCAATACAATTAAAGACTTCATGGACTTATCCAACTTACCAAAGATTACACGTGTAATG
AAAGAAGAAACTTATTATAGTTTTAGAGAATATATTTTAGGTAATAGTTCAAATGTTGTT
GATCCGGATAAATACCGCTTACCAAAATCTATTTCTGCTGAAACCACATTTAATACAAAT
ATGGATGAACCGTCCGTTATATTAGACATCATCATGTCACAACTAGATGAATCCTTAGTT
AGACTAAAAAAATATAACATGCTTACAAAAACAGTTGGATTTAAACTCAAATCCAGTGAT
TTTAAATCCATTACCCGTTCTAAAACACTAGAAGATTATACAAATGATCCTGAAATTATC
AAATATGAACTGACATCACTTTTTGAAAATGAATTTGAAGGTGGGATTTATCGTTTAGCT
GGTGCATCATTAAGTAACTTAATTTTACACAGTGATAATGAAATTCCTTTTAATCTGTTC
ACGTATGAAAAGTTTATCACTAAATTATGA

>protein sequence
LKKSYKVIFHIDLNAFYATCAMIKEPHLKHRVFVVGGQSSQPRGVVSTASYKARKYGIHA
GMSMLDALNKFPRLLIVPVDFKFYREMSNKFMEVLDAYSDLVLQASIDEAFLDMTKASEE
IHPIKLAKKIQKELVDKYQLPTSIGIAPTLFLAKMASDMKKPLGITVLRKRDVEKLLYPL
SVTDIYGIGKQTYPKLMALGINTIKDFMDLSNLPKITRVMKEETYYSFREYILGNSSNVV
DPDKYRLPKSISAETTFNTNMDEPSVILDIIMSQLDESLVRLKKYNMLTKTVGFKLKSSD
FKSITRSKTLEDYTNDPEIIKYELTSLFENEFEGGIYRLAGASLSNLILHSDNEIPFNLF
TYEKFITKLW