ACL_RS02415

Uniprot: A9NFH1
Description: deoxyribonuclease IV
EC number: 3.1.21.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. {ECO:0000255|HAMAP-Rule:MF_00152}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|HAMAP-Rule:MF_00152}; ; Note=Binds 3 Zn(2+) ions. {ECO:0000255|HAMAP-Rule:MF_00152}
Enzyme regulation:
Function [CC]: FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin. {ECO:0000255|HAMAP-Rule:MF_00152}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 71 71 Zinc 1. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 111 111 Zinc 1. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 148 148 Zinc 1. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 148 148 Zinc 2. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 182 182 Zinc 2. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 185 185 Zinc 3. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 217 217 Zinc 2. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 230 230 Zinc 3. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 232 232 Zinc 3. {ECO:0000255|HAMAP-Rule:MF_00152}.; METAL 262 262 Zinc 2. {ECO:0000255|HAMAP-Rule:MF_00152}.
Nucleotide binding:
Site:
Gene names (primary): nfo
Gene names (synonym):
Mass: 33,121
Subunit structure [CC]:
Gene ontology (GO): deoxyribonuclease IV (phage-T4-induced) activity [GO:0008833]; DNA binding [GO:0003677]; zinc ion binding [GO:0008270]; DNA repair [GO:0006281]
Gene ontology IDs: GO:0003677; GO:0006281; GO:0008270; GO:0008833
Chain: CHAIN 1 294 Probable endonuclease 4. /FTId=PRO_1000076798.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the AP endonuclease 2 family. {ECO:0000255|HAMAP-Rule:MF_00152}.
Protein families: AP endonuclease 2 family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFH1
MEROPS:
EnsemblBacteria KO: ABX81101
UniPathway: K01151
CDD:
Gene3D:
HAMAP: 3.20.20.150
InterPro: MF_00152
PANTHER: IPR001719;IPR018246;IPR013022
PIRSF: PTHR21445
PRINTS:
PROSITE:
Pfam: PS00729;PS00730;PS00731;PS51432
ProDom: PF01261
SMART:
SUPFAM: SM00518
TIGRFAMs: SSF51658

506193-507075(+)

>nucleotide sequence
CTGATCTTAGGAAGTCACGTAAGTCTCAAGGGGAATGATATGTTCTATGGTAGTGTATTA
GAGGCACTCGAATACAATGCAAATACCATGATGGTATATACCGGAGCCCCACAAAATACA
ATTAGAAAACCAATTGAAACAATGAAAATAGAAGAGGCGCATGCACTCATGAAGGAAAAA
GGTATTGATTTAAATCATGTCGTGGTGCATGCACCTTACATTATTAATTTATGTAACCCA
GATCCAGAACGTCGTGCATTTGCTGTTGAGTTTCTTACAAAAGAAGTCATTCGTGTTGGA
CAAATGGGCATTAACCAAATGGTTTTACACCCGGGTTCAGCAGTTGGTGGTAATAGAGAA
GAAGCGGTTCAATGGATTAGTGAAGGCTTAAATCAAATTATCGATAACACTAAATCATAC
AACGTCAGAATTGCTTTAGAAACGATGGCTGGAAAAGGCAATGAAATTGGTAAAACCTTT
GAAGAAATCAAAGCAATCATAGATGGCGTTGATGATAAATCAAGAGTATCTGTTTGTTTT
GATACATGTCACGTACATGATGCAGGTTATGATGTCATTCATGATCTAGATAAAACACTC
CAATTATTTGATGATATTGTAGGATTAAACTATATTTCTGTAGTCCACGTAAACGACTCT
AAAAATGAATTAGGTGCTTCCAAAGACCGTCATGAAAATATCGGTTTTGGCTATATAGGT
TATGACGCTTTACTAAAAGTGATTTATCATGAAGCATTTAAAGAGATTCCTAAAATACTA
GAAACTCCGTATGTAAATGATAAACCACCATACAAATTAGAAATTCAAATGATTAAAGAT
AGAGTATTTGATAACGAATTAAAAACAAAGCTAGAAAAATAA

>protein sequence
LILGSHVSLKGNDMFYGSVLEALEYNANTMMVYTGAPQNTIRKPIETMKIEEAHALMKEK
GIDLNHVVVHAPYIINLCNPDPERRAFAVEFLTKEVIRVGQMGINQMVLHPGSAVGGNRE
EAVQWISEGLNQIIDNTKSYNVRIALETMAGKGNEIGKTFEEIKAIIDGVDDKSRVSVCF
DTCHVHDAGYDVIHDLDKTLQLFDDIVGLNYISVVHVNDSKNELGASKDRHENIGFGYIG
YDALLKVIYHEAFKEIPKILETPYVNDKPPYKLEIQMIKDRVFDNELKTKLEK