ACL_RS02485

Uniprot: A9NFI4
Description: acetate kinase
EC number: 2.7.2.1
Annotation score: 4 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + acetate = ADP + acetyl phosphate. {ECO:0000255|HAMAP-Rule:MF_00020}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00020}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_00020}; ; Note=Mg(2+). Can also accept Mn(2+). {ECO:0000255|HAMAP-Rule:MF_00020}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. {ECO:0000255|HAMAP-Rule:MF_00020}.
Pathway: PATHWAY: Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. {ECO:0000255|HAMAP-Rule:MF_00020}.
Active site: ACT_SITE 146 146 Proton donor/acceptor. {ECO:0000255|HAMAP-Rule:MF_00020}.
Binding site: BINDING 14 14 ATP. {ECO:0000255|HAMAP-Rule:MF_00020}.; BINDING 89 89 Substrate. {ECO:0000255|HAMAP-Rule:MF_00020}.
Calcium binding:
DNA binding:
Metal binding: METAL 7 7 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00020}.; METAL 379 379 Magnesium. {ECO:0000255|HAMAP-Rule:MF_00020}.
Nucleotide binding: NP_BIND 204 208 ATP. {ECO:0000255|HAMAP-Rule:MF_00020}.; NP_BIND 279 281 ATP. {ECO:0000255|HAMAP-Rule:MF_00020}.; NP_BIND 327 331 ATP. {ECO:0000255|HAMAP-Rule:MF_00020}.
Site: SITE 178 178 Transition state stabilizer. {ECO:0000255|HAMAP-Rule:MF_00020}.; SITE 237 237 Transition state stabilizer. {ECO:0000255|HAMAP-Rule:MF_00020}.
Gene names (primary): ackA
Gene names (synonym):
Mass: 42,933
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00020}.
Gene ontology (GO): cytoplasm [GO:0005737]; acetate kinase activity [GO:0008776]; ATP binding [GO:0005524]; magnesium ion binding [GO:0000287]; acetyl-CoA biosynthetic process [GO:0006085]; organic acid metabolic process [GO:0006082]
Gene ontology IDs: GO:0000287; GO:0005524; GO:0005737; GO:0006082; GO:0006085; GO:0008776
Chain: CHAIN 1 393 Acetate kinase. /FTId=PRO_1000074180.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the acetokinase family. {ECO:0000255|HAMAP-Rule:MF_00020}.
Protein families: Acetokinase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFI4
MEROPS:
EnsemblBacteria KO: ABX81114
UniPathway: K00925
CDD:
Gene3D:
HAMAP:
InterPro: MF_00020
PANTHER: IPR004372;IPR000890;IPR023865
PIRSF: PTHR21060
PRINTS: PIRSF000722
PROSITE: PR00471
Pfam: PS01075;PS01076
ProDom: PF00871
SMART:
SUPFAM:
TIGRFAMs:

518941-520123(+)

>nucleotide sequence
ATGAAAATTTTATCGGTTAACGCTGGAAGTTCCAGTTTAAAGTTCCAATTATTACAAATG
CCAGAAGAATTTGAAATCGCATCTGGTTTAGTTGAAAGAATTGGTAATAAAAATGCAGAA
TTTAAAATTAAATTTAACGGCTCATCTAAAACTCAAAGTGATTTAGTCGTTTTAGATCAC
TCAGTAGCTGTTGATTTAGTTATCAAAGGTTTACTAGAAAATAAGGTGATTGCTTCTTTA
GATGAAATTCAAGGCGTTGGACACCGTGTTGTTCAAGGTGGACAATTATTTAAAGAATCT
GTAGTCATTGATGATGATGTGGTTATCAAAATTGCATCTTTAAATGACTTGGCACCACTT
CATAATCCAGCAAACATTATTGGTATTAATGCTTTTAGAAAAGCGATATCAAATGTAATT
CATGTTGCAGTGTTTGATACAACATTCCATCAAACTATGCCAGAAGAAAACTTCTTATAT
GGTACACCATATGAGTGGTATACAAAACATGGTGTTAGAAAATATGGTTTCCACGGTACA
TCACACCAATATGTATCTGAACTTGCACATGCACGTTTAGGCAAAGATCATTCAAGAATA
ATTGTTGCTCATATCGGTAATGGTGCATCTATTACAGCTGTAAGAGATGGTAAGAGTATT
GATACATCTATGGGCTTAACACCACTTGAAGGCTTCCCAACGGGAACACGTAGTGGTAAT
GTAGACCCAGCAATCTTCCAATTAATGCAACAAAAAGAAGGTTATTCATTAGAAAAAACT
TTAAACGAGTTAAATAAAAATAGTGGATATCTAGGTATTTCAGGGGTTTCACATGACTCT
AGAGATATTATTGCTGCAGCAGAAGGCGGCAACAAACGTGCTCAGTTAGCACTTGATATT
CAAGCTAAACGTATTGTTGATTACATTGCATCCTACTATGTTTTACTAGGTGGTTTAGAT
GCTTTAGTATTTACTGCAGGTATTGGAGAAAATGCAAAGGAAGTTAGAAAACTTATTTGT
GACAGACTTGGTGTATTAGGCATCAAACTTGATGATAATAAGAATAATTCAAGAGGCGAT
AAAGAAATTTCTAGTGATGCTTCAAATGTTAAAATTTGGGTTATTCCTACAAATGAAGAA
GTCATGATCGCTCGTGATGTATTAAGATTACAAAACAAATAG

>protein sequence
MKILSVNAGSSSLKFQLLQMPEEFEIASGLVERIGNKNAEFKIKFNGSSKTQSDLVVLDH
SVAVDLVIKGLLENKVIASLDEIQGVGHRVVQGGQLFKESVVIDDDVVIKIASLNDLAPL
HNPANIIGINAFRKAISNVIHVAVFDTTFHQTMPEENFLYGTPYEWYTKHGVRKYGFHGT
SHQYVSELAHARLGKDHSRIIVAHIGNGASITAVRDGKSIDTSMGLTPLEGFPTGTRSGN
VDPAIFQLMQQKEGYSLEKTLNELNKNSGYLGISGVSHDSRDIIAAAEGGNKRAQLALDI
QAKRIVDYIASYYVLLGGLDALVFTAGIGENAKEVRKLICDRLGVLGIKLDDNKNNSRGD
KEISSDASNVKIWVIPTNEEVMIARDVLRLQNK