ACL_RS02655

Uniprot: A9NFL7
Description: glycogen-branching enzyme
EC number: 2.4.1.18
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. {ECO:0000256|SAAS:SAAS00554232}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Glycan biosynthesis; glycogen biosynthesis. {ECO:0000256|SAAS:SAAS00046749}.
Active site: ACT_SITE 311 311 Nucleophile. {ECO:0000256|PIRSR:PIRSR000463-1}.; ACT_SITE 354 354 Proton donor. {ECO:0000256|PIRSR:PIRSR000463-1}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): glgB
Gene names (synonym):
Mass: 72,750
Subunit structure [CC]:
Gene ontology (GO): 1,4-alpha-glucan branching enzyme activity [GO:0003844]; cation binding [GO:0043169]; hydrolase activity, hydrolyzing O-glycosyl compounds [GO:0004553]; glycogen biosynthetic process [GO:0005978]
Gene ontology IDs: GO:0003844; GO:0004553; GO:0005978; GO:0043169
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. {ECO:0000256|SAAS:SAAS00554448}.
Protein families: Glycosyl hydrolase 13 family, GlgB subfamily
Coiled coil:
Domain [FT]: DOMAIN 155 502 Aamy. {ECO:0000259|SMART:SM00642}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFL7
MEROPS:
EnsemblBacteria KO: ABX81147
UniPathway: K00700
CDD:
Gene3D:
HAMAP: 2.60.40.10;2.60.40.1180;3.20.20.80
InterPro:
PANTHER: IPR006048;IPR006407;IPR015902;IPR006047;IPR004193;IPR013780;IPR013781;IPR017853;IPR013783;IPR014756
PIRSF: PTHR10357
PRINTS: PIRSF000463
PROSITE:
Pfam:
ProDom: PF00128;PF02806;PF02922
SMART:
SUPFAM: SM00642
TIGRFAMs: SSF51445;SSF81296

561316-563179(+)

>nucleotide sequence
TTGGGGAGGTACATAAAATTGGCATTTACAGAATCTCATGCAAATTTATATCTATTAGGC
AAGAACTATTTAGCACATCAGTTTTTAGGTGCACACCTGAATAAGGATGATGATGGCAAC
ATCATATCTACTACATTTACTGTTTATGCACCCAACGCAAAAGAAGTTAGACTTATTGCA
GATTTCAACAATTACGAAGGCTGGAAGCATGTTCTAACTAAAATTCATCCACAAGGATTT
TGGTCCATTACAATACCCAAAGACCTTGAATGGGCAACTTATAAATATGAGATTCATCTA
AAGAATGGAAAGATCTCACATAAATCAGATCCGTTTGGATACTTTTCTGAAGTAAGACCA
TCAAACGCATCTAAAGTTTATGATATTGATGGGTATCATTGGAAGGATCATGACTGGTTT
AAAAACAAGAAAAAAGTCTATGATCAACCAATGCTTATCTATGAAGTGCATCTTGGCTCT
TGGCAAAGAAATAATGGTCAATTTAGACCGTATAATGAAGTGGTTAAAGATTTAATTGAC
CATGTAAAATACAACGGATTTACCCATATTGAACTCATGCCAGTCTATGAACATCCACTG
GATGACTCATGGGGGTATCAAGGTACTGGTTTTTTTGCGGCAACTTCTAGATTTGGGTCA
CCAAAAGATTTAATGTATTTAATCGATCAGTGTCATCAACATGGTATTGGTATCATCATG
GACTGGGTTTTAGGACATATTAATAAAGATGACTTTGGTTTATTTAATTTTGATGGCACT
TATCTTTATGAATATGAGGATACCTTTAAAAGAGAAAACATAACATGGGGTACTGCGAAT
CTAGACTTCAATAAAGGTATTACTAAAAGTTTTATGATGTCTGCCCTTACTTTTTGGATG
GATTACTTCCATGTTGATGGATTTAGAATTGATGCTGTATCTCACCTGTTTTATTATCTA
GGTGATGAGTCCAATGGTATCAATGAAGAAGCTATTGCATTTTTAAAAGAAATTTCAGCA
CATGTATTTAGTAAGGATGAAACCTTCATCCTATCTGCAGAAGACTCAACGGCATTTCCA
CTCGTTACCAAACCAACCTTTCTTGGTGGTATTGGGTTCAACTATAAATGGAATATGGGC
TTTATGAATGATGTCTTATCTTACTTTAAACTTGATCCGATATACAGGAAACATAACCAT
GATAAACTAACTTTTGGCTTAGTTTACGCATTTAATGAACAATTTATTTTACCCTTTAGC
CATGATGAGGTCGTACACCTAAAAGGTTCCTTAGTCAGTAAAATGCCGGGAAGTTATGAA
CAAAAGTTTGCGCATTATAGAAACCTTATGACATTCTATATGACACACCCGGGTAAAAAG
TTACTATTTATGGGTCAGGAATTTGCACAAATTGCTGAATGGGATTTTAAAAAGGAACTT
GACTGGAACTTATTAAAGTTTCCGATACATGACGCTTTTAATAGATACTTTAGGGATTTA
GCACATGTGTATAAACACCATGATGCACTTTATCAAAAAGATCATGATCCGTCAGGATTT
AAATGGTTAATCGTGGATCATAAAGACACATCTGTCTTTGCTTATGCAAGATACTCGGAT
AACGAAGTACTTGTCATGGTTCATAATCTAACTCCAAACTATTATCAAAGCTATGAAGTT
GGTGTACCTTTTGAAGGAACATATGAGGAAATATTAAATTCCAACAAAGACATTTATAAT
GGAACAAACTTATACAACGGCTTACCACTTAAAACCTACAAAGAACCAAGAAATGGTCAA
CCATATCATCTCAAGGTACAACTTTCAGGGTTAACGAGTATTATCTTTAAATATAAAAAA
TGA

>protein sequence
LGRYIKLAFTESHANLYLLGKNYLAHQFLGAHLNKDDDGNIISTTFTVYAPNAKEVRLIA
DFNNYEGWKHVLTKIHPQGFWSITIPKDLEWATYKYEIHLKNGKISHKSDPFGYFSEVRP
SNASKVYDIDGYHWKDHDWFKNKKKVYDQPMLIYEVHLGSWQRNNGQFRPYNEVVKDLID
HVKYNGFTHIELMPVYEHPLDDSWGYQGTGFFAATSRFGSPKDLMYLIDQCHQHGIGIIM
DWVLGHINKDDFGLFNFDGTYLYEYEDTFKRENITWGTANLDFNKGITKSFMMSALTFWM
DYFHVDGFRIDAVSHLFYYLGDESNGINEEAIAFLKEISAHVFSKDETFILSAEDSTAFP
LVTKPTFLGGIGFNYKWNMGFMNDVLSYFKLDPIYRKHNHDKLTFGLVYAFNEQFILPFS
HDEVVHLKGSLVSKMPGSYEQKFAHYRNLMTFYMTHPGKKLLFMGQEFAQIAEWDFKKEL
DWNLLKFPIHDAFNRYFRDLAHVYKHHDALYQKDHDPSGFKWLIVDHKDTSVFAYARYSD
NEVLVMVHNLTPNYYQSYEVGVPFEGTYEEILNSNKDIYNGTNLYNGLPLKTYKEPRNGQ
PYHLKVQLSGLTSIIFKYKKW