ACL_RS02770

Uniprot: A9NFP0
Description: lipoate--protein ligase
EC number: 6.3.1.20
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Predicted
Catalytic activity: CATALYTIC ACTIVITY: ATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N(6)-(lipoyl)lysine + AMP + diphosphate. {ECO:0000256|SAAS:SAAS00603726}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2. {ECO:0000256|SAAS:SAAS00511528}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): lplA1
Gene names (synonym):
Mass: 38,562
Subunit structure [CC]:
Gene ontology (GO): ATP binding [GO:0005524]; ligase activity [GO:0016874]; nucleotidyltransferase activity [GO:0016779]; protein lipoylation [GO:0009249]
Gene ontology IDs: GO:0005524; GO:0009249; GO:0016779; GO:0016874
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Contains BPL/LPL catalytic domain. {ECO:0000256|SAAS:SAAS00514708}.
Protein families:
Coiled coil:
Domain [FT]: DOMAIN 28 214 BPL/LPL catalytic. {ECO:0000259|PROSITE:PS51733}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFP0
MEROPS:
EnsemblBacteria KO: ABX81170
UniPathway: K03800
CDD:
Gene3D:
HAMAP:
InterPro:
PANTHER: IPR004143;IPR019491;IPR004562
PIRSF:
PRINTS:
PROSITE:
Pfam: PS51733
ProDom: PF03099;PF10437
SMART:
SUPFAM:
TIGRFAMs:

588708-589707(+)

>nucleotide sequence
ATGATACTTATAAAACATCATAATGAAGGTAATTTAGAACCATACTTTTATTTTGCATTA
GAAAAATATGTATTAGAACATTTACTTAAAGACAATGAAACCTATTTCTTCACCTGGAGA
ATAAAAGGCATTGTTTCAGGTAAGAACCAAGTCATTGAAAATGAAATTAATATTCCCTAT
GTCAAAGAACATAATGTGAAATTCTTTAGAAGACCCACTGGTGGCGGATCTGTCTATGCA
GATGAAAATAACACCATGTTTACAATTATTACCAAAAGAAGTGGACAAAACTTCTCATTT
AAAAACTACTTAGTCAAAATCACTGATGCATTTAAAAAACTAGGTGTTAATCTAGATTTT
AGTGGTAGAAATGATATCTTATTAGATGGTAAAAAGGTATCTGGTAATGCATTCTTACAA
AACAAGTATGGTATGTTAATGCATGGAACTATGATGTATGATTTAGATATTGAAACGATG
GTGCGTGCATTAAATCCACCGGATGAGAAACTTATTTCTAAAGGTATTGCAAGTGTAAGA
AGTCGTGTAAACAATCTAAAACCTTATTTAAATGGAATGAGTCATGATGAATTAGTTAGT
TATCTTGAAACAGAACTAACAAGCAGTGTTTATGAAATCAATCAAGAAGAAGTAGAACAA
CTTAAAAAAGATGCTAAATTTTATGCAAGTTCAGAATGGCTATACCGTTCTCAACCAGAA
TATACTCAAAAGCTATCTAAACGATTTGATTGGGGACATATCGATATGCGTTTTAACATC
AAATCAGGTATGATTCAAAACGTAGATATCACTGGAGATTTCTTTGAGCTCAAAGAAGAT
TTAAAATCCCTTGAAAAACCATTATTAAACCAAGTTTGGAGCTATGCATCCATTAAAGAA
ATTTTAAACAGTATCGAAGTAGATAAGATCATTTTAAACGCTACAAATGAAGATATCTTA
GCACTCTTTGATAATGCTTTTATAGAGACAAATAACTAA

>protein sequence
MILIKHHNEGNLEPYFYFALEKYVLEHLLKDNETYFFTWRIKGIVSGKNQVIENEINIPY
VKEHNVKFFRRPTGGGSVYADENNTMFTIITKRSGQNFSFKNYLVKITDAFKKLGVNLDF
SGRNDILLDGKKVSGNAFLQNKYGMLMHGTMMYDLDIETMVRALNPPDEKLISKGIASVR
SRVNNLKPYLNGMSHDELVSYLETELTSSVYEINQEEVEQLKKDAKFYASSEWLYRSQPE
YTQKLSKRFDWGHIDMRFNIKSGMIQNVDITGDFFELKEDLKSLEKPLLNQVWSYASIKE
ILNSIEVDKIILNATNEDILALFDNAFIETNN