ACL_RS02800

Uniprot: A9NFP6
Description: NAD+ synthase glutamine-dependent
EC number: 6.3.1.5
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): nadE
Gene names (synonym):
Mass: 68,689
Subunit structure [CC]:
Gene ontology (GO): ATP binding [GO:0005524]; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds [GO:0016810]; NAD+ synthase (glutamine-hydrolyzing) activity [GO:0003952]; NAD+ synthase activity [GO:0008795]; NAD biosynthetic process [GO:0009435]
Gene ontology IDs: GO:0003952; GO:0005524; GO:0008795; GO:0009435; GO:0016810
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the NAD synthetase family. {ECO:0000256|RuleBase:RU003811}.
Protein families: NAD synthetase family
Coiled coil:
Domain [FT]: DOMAIN 7 287 CN hydrolase. {ECO:0000259|PROSITE:PS50263}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NFP6
MEROPS:
EnsemblBacteria KO: ABX81176
UniPathway: K01950
CDD:
Gene3D: cd00553
HAMAP: 3.40.50.620;3.60.110.10
InterPro:
PANTHER: IPR003010;IPR014445;IPR022310;IPR003694;IPR014729
PIRSF:
PRINTS: PIRSF006630
PROSITE:
Pfam: PS50263
ProDom: PF00795;PF02540
SMART:
SUPFAM:
TIGRFAMs: SSF56317

593368-595219(+)

>nucleotide sequence
ATGACACAACACGGTATATTAAAAGTTTCACTTGCAAGTCCTGAAATCAAATTAGGTAAT
CCACTGCTCAATGTGGAACCTATTTTAAATATACTTAATCATGAATCCTCTGGGTTAGTT
TTATTTCCTGAACTATCATTAACTGGTTACACCTCAGGAGATTTATTTTTCCAATTAGAT
TACATCAATAAAACAAATGAAGCACTAAGTAAAATTATGAATGGAACAACTTATCAAGGT
GTGTACATATTAGGTATGCCTTTAAAAGTAGGGGATGCACTTTTTAATGTTGCAGTAGTT
ATTCAAAATAAGAAGATACTTGGTGTGATACCTAAGTATCACCTACCAAATTCAAAAGAG
TTTAATGAAAAGCGCTGGTTCCAATCAGGTTTTAACTATGAGCTTGATACAGTGGAAGTA
CTTGGACAAACCGTCCCATTTGGTGATATGGTCTTTAAAGATGAATTACATGACATTGGA
TTTGGTGTAGAAATATGCCAAGATATGTGGGCGATTGAGACACCTGGAAATCATTTAAGT
CTAAGTGGTGCTCATCTGATTTTAAACTTATCGGCCTCACCTGAAACCATGGGTAAACAA
ACGGTTAGAAAACACACTGTATTAGATGCTTCAAGAAGACAGTTAAGTGGTTATTTATAC
ACTGCTTCTGCATCATCAGAATCAACAATGGATGTCGTGTTTACACCACATAAGATAGCA
GCTAGTTTGGGTAGTTTGATTATTGAATCTAGTCTATTAGATCCTAAAGAAGTTTTAAGA
GTAGACATGGACATAGAAGCTATTCGCTATCAAAGAAGAATTGATTCTACCTTTAAAGAT
GAACAAAATGGCTATACACAATCAACAACTAAAGTTCATGTAGACTTTTTTACTAACAAT
GACTATGTATTTGAAAGACCTTTAAACAAAACACCATTCCTACCTAAAGTAGGCGATAAA
GAAAGTTTACTGATTGCTAATCAAATACAAGTACAAGGTTTAGTTCAAAAAATCAAACAT
CTAGGAAAACCTAAGATTATTATTGGTATCTCAGGTGGTTTGGATAGTACACTTGCTTTA
TTAGTTGCTCATCAAGCAATTAAAAAACTAAATGGTGATGTTAAAGATATCATCGGTGTT
ACTATGCCTAGTCAAGTAACCTCTAAAGGTTCTAAGACAGATGCCATCTTACTCATGGAA
AAATTAGGTATCACAGCACTTGAAATTCCAATTGAAGATCAAGTAAAACTTCATCTTGAA
AGTTTAAATCATGGTGGTAGTACAGATATTACCTATGAAAATGCACAGGCTAGAATCCGT
ACACTCATATTAATGAATCTATCAAACAAACACCAAGGATTTGTTTTAGGTACAGGTGAC
TTAAGCGAAATAGCGCTTGGTTGGATGACTTTTAATGGTGATCATATGAGTATGTATAAT
GTCAATGCTGGTGTACCTAAAACACTTGTTCAAGCACTTGTTAAGCTGCACAGTGAACAT
AACTACCAAGATATTAAAGATATATTAATAAGTATTTTAAACCGACCAATCAGCCCAGAA
TTAAAAGAAAATCAAAAAACTGAGGATGTCATTGGTACATATGAAATTAATGATTTTATG
CTGCATCATTTATTAGAAAATGGTGCGAGTGAAGAAAAATTATCCTTCCTAGTTAGACAC
ACATTTGGTCTATCTGTAGAAGCATCAGATCTTTATGCAAAAAGATTATTAAACAGGTTT
TATCAGTCACAGTTTAAGCGTCAAGTGATGCCTGAGGGACCAAAAGTGTTAAGGATAAGC
TTATCACCTAGAGGTGATTTACGCTTACCTTCAGATATGAATAGAAAGTAG

>protein sequence
MTQHGILKVSLASPEIKLGNPLLNVEPILNILNHESSGLVLFPELSLTGYTSGDLFFQLD
YINKTNEALSKIMNGTTYQGVYILGMPLKVGDALFNVAVVIQNKKILGVIPKYHLPNSKE
FNEKRWFQSGFNYELDTVEVLGQTVPFGDMVFKDELHDIGFGVEICQDMWAIETPGNHLS
LSGAHLILNLSASPETMGKQTVRKHTVLDASRRQLSGYLYTASASSESTMDVVFTPHKIA
ASLGSLIIESSLLDPKEVLRVDMDIEAIRYQRRIDSTFKDEQNGYTQSTTKVHVDFFTNN
DYVFERPLNKTPFLPKVGDKESLLIANQIQVQGLVQKIKHLGKPKIIIGISGGLDSTLAL
LVAHQAIKKLNGDVKDIIGVTMPSQVTSKGSKTDAILLMEKLGITALEIPIEDQVKLHLE
SLNHGGSTDITYENAQARIRTLILMNLSNKHQGFVLGTGDLSEIALGWMTFNGDHMSMYN
VNAGVPKTLVQALVKLHSEHNYQDIKDILISILNRPISPELKENQKTEDVIGTYEINDFM
LHHLLENGASEEKLSFLVRHTFGLSVEASDLYAKRLLNRFYQSQFKRQVMPEGPKVLRIS
LSPRGDLRLPSDMNRK