ACL_RS02805

Uniprot: A9NFP7
Description: tRNA 2-thiouridine(34) synthase MnmA
EC number: 2.8.1.13
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor. {ECO:0000255|HAMAP-Rule:MF_00144}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. {ECO:0000255|HAMAP-Rule:MF_00144}.
Pathway:
Active site: ACT_SITE 109 109 Nucleophile. {ECO:0000255|HAMAP-Rule:MF_00144}.; ACT_SITE 205 205 Cysteine persulfide intermediate. {ECO:0000255|HAMAP-Rule:MF_00144}.
Binding site: BINDING 34 34 ATP; via amide nitrogen and carbonyl oxygen. {ECO:0000255|HAMAP-Rule:MF_00144}.; BINDING 133 133 ATP; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_00144}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 8 15 ATP. {ECO:0000255|HAMAP-Rule:MF_00144}.
Site: SITE 134 134 Interaction with tRNA. {ECO:0000255|HAMAP-Rule:MF_00144}.; SITE 346 346 Interaction with tRNA. {ECO:0000255|HAMAP-Rule:MF_00144}.
Gene names (primary): mnmA
Gene names (synonym):
Mass: 43,315
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; sulfurtransferase activity [GO:0016783]; tRNA binding [GO:0000049]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0000049; GO:0005524; GO:0005737; GO:0006400; GO:0016783
Chain: CHAIN 1 375 tRNA-specific 2-thiouridylase MnmA. /FTId=PRO_0000349495.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the MnmA/TRMU family. {ECO:0000255|HAMAP-Rule:MF_00144}.
Protein families: MnmA/TRMU family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 104 106 Interaction with target base in tRNA. {ECO:0000255|HAMAP-Rule:MF_00144}.; REGION 155 157 Interaction with tRNA. {ECO:0000255|HAMAP-Rule:MF_00144}.; REGION 313 314 Interaction with tRNA. {ECO:0000255|HAMAP-Rule:MF_00144}.
EMBL: CP000896
ProteinModelPortal: A9NFP7
MEROPS:
EnsemblBacteria KO: ABX81177
UniPathway: K00566
CDD:
Gene3D:
HAMAP: 2.30.30.280;3.40.50.620
InterPro: MF_00144
PANTHER: IPR023382;IPR014729;IPR004506
PIRSF: PTHR11933
PRINTS:
PROSITE:
Pfam:
ProDom:
SMART:
SUPFAM:
TIGRFAMs:

595226-596354(+)

>nucleotide sequence
TTGAAAAAGAAAGTATTAATCGGACTATCAGGCGGAGTCGACTCTTCAGTCGCTGCTTTG
ATACTGTTACAACAAGGCTATGATGTAGAAGCGGTATTCATGCGTAATTGGGATTCAGCA
ACAAATATGGATTTTAGAGGCAACCCAACGTTATATGATGAAACATGTGAACAAGAAAAA
GATTACCAAGATGCATTAAAAGTAGCAAATAAATTGGGTATTAAATTACATAGAGTAGAT
TTTATTCATGAATACTGGGATCGTGTTTTTTCATATTTCATTGATGAATATGAAAAGAAT
AGAACACCAAATCCTGACGTATTATGTAATAATGAAATAAAATTTAAAGCATTTATCGAT
TATAGTGAAAAGTTTAAACCAGACTATATTGCAATGGGTCACTATGCTCAAATTGATCAT
ACAGGTGATGAACCAAAACTAATTAGAGCAGTAGATTCTAATAAAGATCAAACATACTTT
TTAAGTCAACTTAAAACAGAACAATTAAGAAATGTCTTATTCCCAATTGGTAATTTACCA
AAGAGTGAAGTTAGAAGAATTGCTAAAGAGCATGATCTAGCTACAGCTGATAAAAAAGAC
TCTACCGGTATTTGTTTTATTGGTGAACGTCATTTTAATGAATTCTTACTAAACTACCTA
CCTGCAAAAGAAGGCGATATGAGACGTTTAGATGGCACTTTTATTAAACGTCACTTTGGT
TTAATGAACTATACAATTGGTCAAAGAAAAGGCTTAGGTATCGGTGGTACAACCGATACA
ACAGATGCTTGGTTTGTTGTAGGTAAAGACTTATCTACAAACACACTGTATGTTGAACCA
GGTTTTGAGCATCCTTATTTATATTCAGATGAAGCATTAATTACTGAAGTTAAGTGGCGT
GGAGCAAAAAGAGATGGTAAATTTACAGCGAAATTCAGATATCGCCAAGAAGACCAAGAT
GTTGAAATTAAATGGATTGATGACGAAACATTTAAAGTCTTTTATCCTCAAAAAATACGT
GCAGTAACACCGGGTCAAGTGTGTGCGATTTATGAAGATGAAGTGTGTGTTGGTTCAGGA
TTTATTTCAGTAGTATACAATGAAGGGGAAAAAAGATTATATAGCTAA

>protein sequence
LKKKVLIGLSGGVDSSVAALILLQQGYDVEAVFMRNWDSATNMDFRGNPTLYDETCEQEK
DYQDALKVANKLGIKLHRVDFIHEYWDRVFSYFIDEYEKNRTPNPDVLCNNEIKFKAFID
YSEKFKPDYIAMGHYAQIDHTGDEPKLIRAVDSNKDQTYFLSQLKTEQLRNVLFPIGNLP
KSEVRRIAKEHDLATADKKDSTGICFIGERHFNEFLLNYLPAKEGDMRRLDGTFIKRHFG
LMNYTIGQRKGLGIGGTTDTTDAWFVVGKDLSTNTLYVEPGFEHPYLYSDEALITEVKWR
GAKRDGKFTAKFRYRQEDQDVEIKWIDDETFKVFYPQKIRAVTPGQVCAIYEDEVCVGSG
FISVVYNEGEKRLYS