ACL_RS03520

Uniprot: A9NG43
Description: tRNA guanosine(34) transglycosylase Tgt
EC number: 2.4.2.29
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine. {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777, ECO:0000256|SAAS:SAAS00087626}.; CATALYTIC ACTIVITY: Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine. {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777, ECO:0000256|SAAS:SAAS00087703}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777}; ; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777}
Enzyme regulation:
Function [CC]: FUNCTION: Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777, ECO:0000256|SAAS:SAAS00383991}.
Pathway: PATHWAY: tRNA modification; tRNA-queuosine biosynthesis. {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|SAAS:SAAS00087869}.
Active site: ACT_SITE 98 98 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_00168}.
Binding site: BINDING 99 99 Substrate. {ECO:0000256|HAMAP-Rule:MF_00168}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): tgt
Gene names (synonym):
Mass: 42,610
Subunit structure [CC]:
Gene ontology (GO): metal ion binding [GO:0046872]; queuine tRNA-ribosyltransferase activity [GO:0008479]; queuosine biosynthetic process [GO:0008616]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0006400; GO:0008479; GO:0008616; GO:0046872
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family. {ECO:0000256|HAMAP-Rule:MF_00168, ECO:0000256|RuleBase:RU003777, ECO:0000256|SAAS:SAAS00571098}.
Protein families: Queuine tRNA-ribosyltransferase family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NG43
MEROPS:
EnsemblBacteria KO: ABX81323
UniPathway: K00773
CDD:
Gene3D:
HAMAP: 3.20.20.105
InterPro: MF_00168
PANTHER: IPR004803;IPR002616
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF01702
SMART:
SUPFAM:
TIGRFAMs: SSF51713

739068-740202(-)

>nucleotide sequence
TTAGTAACTTACTTTAGGATAATTCGTTCTTTCATAAAACTCTTTTTTAAAATCAAGTAA
TCTGTCCTCTTTAATTGCTTGTCTAATTTCTGCCATTAAGTGCTTTAAATATGCTAAATT
TTGGATAGTTACTAGTCTTTGACCTAGTTGTTCTTCTGCTTTAAATAAGTGTCTAATATA
ACTCTTAGAGTACTTTGAAACACTTGATTGTAGATTTGGATCTAGACTTGACATGTCACG
TTCAAATTGTTTATTTTTAATAGTCACTTTACCGGTAGTTGTAAGTGCTGTACCATGTCT
AGCATTTCTAGTAGGCAGTACACAGTCAAACATATCTACACCATTAATGACGTTTTCTAG
TAAGTCCTCAGGTGCACCCACACCCATTAAGTAACGCGGTTTATTAAACGGTAAAATCGG
GTTTAAATGTCTTGTCATCTCATACATTTCAGCTTTTGTTTCACCAACGGATAAGCCACC
AATAGAATAACCTGGAAAATCCAGTTTAATGAGTTCTTCAGCACAGTGAGTTCTTAATTC
TTTATTTAAACCACCTTGTACAATTCCAAATAAAGCTTGATCAGTTTTTAATGCTTCTTT
ACCTCTTTTTGCCCAACGAATCGTTCTTTCTACAGAATCCTTCATATATTCATAGGATTC
ATAAGGTGGTGGACATTCATCAAAAGACATAATAATATCCGCACCTAAGTCTTCTTGAAT
GCGTATCGCATCTTCTGGACCTAAGAATAATGGAGCACCAGATTTATGATGCTTGAAATG
AACACCTTCTTCTTTAATATCACGTAATTTAGCAAGTGAAAATACTTGAAAACCACCGGA
ATCCGTTAAAAGTGCGCCATCCCATTTCATAAATCCACGAATACCACCATGATCTTTTAC
GATATCTTCACCTGGTTGTTGCCATAGGTGATAGGTGTTTGCTAAGATTAAACCTTCTGA
AACTTCTTTAATTTCTTCAACTGTTAATGTTTTTACAGTAGCGAGTGTACCTACTGGCAT
AAAAATAGGTGTTTCAAACACACCATGTGATGTCGTTAATTTACCAAGCCTAGCACCACT
TTGCTTACAAACATGTAATACTTCATATGAAATCAAAATAAAACCACCTTCCAT

>protein sequence
MEGGFILISYEVLHVCKQSGARLGKLTTSHGVFETPIFMPVGTLATVKTLTVEEIKEVSE
GLILANTYHLWQQPGEDIVKDHGGIRGFMKWDGALLTDSGGFQVFSLAKLRDIKEEGVHF
KHHKSGAPLFLGPEDAIRIQEDLGADIIMSFDECPPPYESYEYMKDSVERTIRWAKRGKE
ALKTDQALFGIVQGGLNKELRTHCAEELIKLDFPGYSIGGLSVGETKAEMYEMTRHLNPI
LPFNKPRYLMGVGAPEDLLENVINGVDMFDCVLPTRNARHGTALTTTGKVTIKNKQFERD
MSSLDPNLQSSVSKYSKSYIRHLFKAEEQLGQRLVTIQNLAYLKHLMAEIRQAIKEDRLL
DFKKEFYERTNYPKVSY