ACL_RS03900

Uniprot: A9NGC1
Description: 4-hydroxy-tetrahydrodipicolinate reductase
EC number: 1.17.1.8
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)(+) + H(2)O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H. {ECO:0000256|HAMAP-Rule:MF_00102}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate. {ECO:0000256|HAMAP-Rule:MF_00102}.
Pathway: PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. {ECO:0000256|HAMAP-Rule:MF_00102}.
Active site: ACT_SITE 137 137 Proton donor/acceptor. {ECO:0000256|HAMAP-Rule:MF_00102}.; ACT_SITE 141 141 Proton donor. {ECO:0000256|HAMAP-Rule:MF_00102}.
Binding site: BINDING 36 36 NADP. {ECO:0000256|HAMAP-Rule:MF_00102}.; BINDING 138 138 Substrate. {ECO:0000256|HAMAP-Rule:MF_00102}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 8 13 NAD(P). {ECO:0000256|HAMAP-Rule:MF_00102}.; NP_BIND 80 82 NAD(P). {ECO:0000256|HAMAP-Rule:MF_00102}.; NP_BIND 104 107 NAD(P). {ECO:0000256|HAMAP-Rule:MF_00102}.
Site:
Gene names (primary): dapB
Gene names (synonym):
Mass: 26,596
Subunit structure [CC]: SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00102}.
Gene ontology (GO): cytoplasm [GO:0005737]; 4-hydroxy-tetrahydrodipicolinate reductase [GO:0008839]; NAD binding [GO:0051287]; NADP binding [GO:0050661]; oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor [GO:0016726]; diaminopimelate biosynthetic process [GO:0019877]; lysine biosynthetic process via diaminopimelate [GO:0009089]
Gene ontology IDs: GO:0005737; GO:0008839; GO:0009089; GO:0016726; GO:0019877; GO:0050661; GO:0051287
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DapB family. {ECO:0000256|HAMAP-Rule:MF_00102, ECO:0000256|SAAS:SAAS00539023}.
Protein families: DapB family
Coiled coil:
Domain [FT]: DOMAIN 2 107 DapB_N. {ECO:0000259|Pfam:PF01113}.; DOMAIN 110 238 DapB_C. {ECO:0000259|Pfam:PF05173}.
Motif:
Region: REGION 147 148 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00102}.
EMBL: CP000896
ProteinModelPortal: A9NGC1
MEROPS:
EnsemblBacteria KO: ABX81401
UniPathway: K00215
CDD:
Gene3D:
HAMAP: 3.40.50.720
InterPro: MF_00102
PANTHER: IPR022663;IPR000846;IPR023940;IPR016040
PIRSF: PTHR20836
PRINTS: PIRSF000161
PROSITE:
Pfam:
ProDom: PF05173;PF01113
SMART:
SUPFAM:
TIGRFAMs: SSF51735

822013-822736(-)

>nucleotide sequence
TTAAGCACCTAGCAAATCATCCATACCAAATAATCCACTCTTCTTATCTTTAATGAATTT
AGCTGCTTTAATAGCACCTTCAGCAAAAACACTCTTTTCATGAGCAATATGTGTAAATTC
TAAAGTCTCACTTAGGTTAGAAAAAATGACAGTATGCTCTCCAACATAGTTTCCTAGTCG
AAGGCTATGTACGTGAACTCCATTTTCACTGGATTCACCATGAATAAGTGCTCTTTTTTC
AGGTAATGCATCATTGACTGCATCATATAGTTTAAACGCTGTGCCACTTGGACTATCTTT
TTTAAAACGGTGATGTTTATCAATCATTTCTATATCATAACTACTATCTATTTGACTGCT
TACAACTTTGAGTGCGTTTGTCAGTAAGTGTATACCAACAGAATAGTTTGCAGAATAAAA
GATAGGTATATGTTGACTATATGATTTTATTAAAAGAAAATCATCTTCACTATAACCTGT
GGTTGCAAGTACTAATGCTTTATGATGTTTTTTGGCAAAGTCTAAAAGTGCAGGTAATAT
AGTAGGTGTGGAAAAATCAATTAACACATCAAAGCTACCTAAGTCATCTAACTTATCTAC
TATTTGTTCTGACCTTTTACGATCATAACCACCAATGTATTCAAGTGTTTCATCTAATAA
TATTTGTTTTTTTAAGACCTGGCCCATTTTACCAAGTGCACCACTAATTGCTATTTTAAT
CAT

>protein sequence
MIKIAISGALGKMGQVLKKQILLDETLEYIGGYDRKRSEQIVDKLDDLGSFDVLIDFSTP
TILPALLDFAKKHHKALVLATTGYSEDDFLLIKSYSQHIPIFYSANYSVGIHLLTNALKV
VSSQIDSSYDIEMIDKHHRFKKDSPSGTAFKLYDAVNDALPEKRALIHGESSENGVHVHS
LRLGNYVGEHTVIFSNLSETLEFTHIAHEKSVFAEGAIKAAKFIKDKKSGLFGMDDLLGA