ACL_RS03905

Uniprot: A9NGC2
Description: 4-hydroxy-tetrahydrodipicolinate synthase
EC number: 4.3.3.7
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O. {ECO:0000255|HAMAP-Rule:MF_00418}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA). {ECO:0000255|HAMAP-Rule:MF_00418}.
Pathway: PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. {ECO:0000255|HAMAP-Rule:MF_00418}.
Active site: ACT_SITE 134 134 Proton donor/acceptor. {ECO:0000255|HAMAP-Rule:MF_00418}.; ACT_SITE 162 162 Schiff-base intermediate with substrate. {ECO:0000255|HAMAP-Rule:MF_00418}.
Binding site: BINDING 46 46 Pyruvate. {ECO:0000255|HAMAP-Rule:MF_00418}.; BINDING 204 204 Pyruvate; via carbonyl oxygen. {ECO:0000255|HAMAP-Rule:MF_00418}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site: SITE 45 45 Part of a proton relay during catalysis. {ECO:0000255|HAMAP-Rule:MF_00418}.; SITE 108 108 Part of a proton relay during catalysis. {ECO:0000255|HAMAP-Rule:MF_00418}.
Gene names (primary): dapA
Gene names (synonym):
Mass: 32,475
Subunit structure [CC]: SUBUNIT: Homotetramer; dimer of dimers. {ECO:0000255|HAMAP-Rule:MF_00418}.
Gene ontology (GO): cytoplasm [GO:0005737]; 4-hydroxy-tetrahydrodipicolinate synthase [GO:0008840]; diaminopimelate biosynthetic process [GO:0019877]; lysine biosynthetic process via diaminopimelate [GO:0009089]
Gene ontology IDs: GO:0005737; GO:0008840; GO:0009089; GO:0019877
Chain: CHAIN 1 293 4-hydroxy-tetrahydrodipicolinate synthase. /FTId=PRO_1000080519.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DapA family. {ECO:0000255|HAMAP-Rule:MF_00418}.
Protein families: DapA family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGC2
MEROPS:
EnsemblBacteria KO: ABX81402
UniPathway: K01714
CDD:
Gene3D: cd00950
HAMAP: 3.20.20.70
InterPro: MF_00418
PANTHER: IPR013785;IPR005263;IPR002220;IPR020625
PIRSF: PTHR12128
PRINTS: PIRSF001365
PROSITE: PR00146
Pfam: PS00666
ProDom: PF00701
SMART:
SUPFAM: SM01130
TIGRFAMs:

822728-823610(-)

>nucleotide sequence
TTAATCATTATCCACCAACTTATAGTCTCTTAAAACTTTTAACAACATACGGTTATGTTT
AGCACCAAGTTTCGTTAAAGGTAATCTTGGTTTACCTACTTCAAATCCTAAATGTTCTAG
TGCTCTTTTAACAGGTATAGGATTGGATTCAACAAACATCATATCATGAAGTTTGTTGAA
TTTTATAAATGCTTCTTTATTATCTATACCAGCTCTAAAGTTTTGAATAAGTAGTGAAGT
AGATAATGGAATAATATTTGCAGTAACAGATATGACACCATGACCACCTAAACTTAAAAC
ATCATAGACTTGGTCATCATTGCCACTTAAGACAATAAAATCTTCATGTGTTTGGTCAAT
AATAGATTTAACTTGTTCTAAGTTACCACTTGCTTCTTTAATACCTATAATATTTTTGAC
TTTACTTAAGCGTTTAACTGTATCTACTTCTAGGTTCACACCGGTTCTAGATGGTACATT
ATAAAGCATAATAGGTAAATTAATTGATTTAGCTACAGCCTTATAGTGCGCTAATAACCC
ATTTTGAGTTGGTTTATTATAGTAAGGTGTAACAATTAATAAAGCATCAGCACCTAATCT
TTGTGCAGTTCTTGAAAAAGATATCGTTTGTTTTGTATCATTACTTCCTGTACCTACCAT
AATAGGTATACGCTTATTAATGTAATTTACTACAAACGAAATAACTTCTTTTTTCTCACT
TGAGGTAAGTGTTGGTGATTCAGCTGTAGTACCTAATATCACTAAAAAATCTGTTTCATT
CTCAATATGAAAATCTAATAATTTTTGTAGTACTGGGTAGTTAATTTTAGACCCCTTAAA
TGGAGTAACTAGTGCAACCCCTGAACCTGTAAATTTTTTCAT

>protein sequence
MKKFTGSGVALVTPFKGSKINYPVLQKLLDFHIENETDFLVILGTTAESPTLTSSEKKEV
ISFVVNYINKRIPIMVGTGSNDTKQTISFSRTAQRLGADALLIVTPYYNKPTQNGLLAHY
KAVAKSINLPIMLYNVPSRTGVNLEVDTVKRLSKVKNIIGIKEASGNLEQVKSIIDQTHE
DFIVLSGNDDQVYDVLSLGGHGVISVTANIIPLSTSLLIQNFRAGIDNKEAFIKFNKLHD
MMFVESNPIPVKRALEHLGFEVGKPRLPLTKLGAKHNRMLLKVLRDYKLVDND