ACL_RS03955

Uniprot: A9NGD2
Description: type 2 isopentenyl-diphosphate Delta-isomerase
EC number: 5.3.3.2
Annotation score: 4 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Isopentenyl diphosphate = dimethylallyl diphosphate. {ECO:0000256|HAMAP-Rule:MF_00354}.
Cofactor: COFACTOR: Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000256|HAMAP-Rule:MF_00354}; ; COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_00354}; ; COFACTOR: Name=NADPH; Xref=ChEBI:CHEBI:57783; Evidence={ECO:0000256|HAMAP-Rule:MF_00354};
Enzyme regulation:
Function [CC]: FUNCTION: Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP). {ECO:0000256|HAMAP-Rule:MF_00354}.
Pathway:
Active site:
Binding site: BINDING 90 90 FMN; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00354}.; BINDING 118 118 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.; BINDING 148 148 Substrate. {ECO:0000256|HAMAP-Rule:MF_00354}.; BINDING 180 180 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.; BINDING 205 205 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.; BINDING 210 210 FMN; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00354}.
Calcium binding:
DNA binding:
Metal binding: METAL 149 149 Magnesium. {ECO:0000256|HAMAP-Rule:MF_00354}.
Nucleotide binding: NP_BIND 60 62 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.; NP_BIND 254 256 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.; NP_BIND 275 276 FMN. {ECO:0000256|HAMAP-Rule:MF_00354}.
Site:
Gene names (primary): idi
Gene names (synonym): fni
Mass: 35,979
Subunit structure [CC]: SUBUNIT: Homooctamer. Dimer of tetramers. {ECO:0000256|HAMAP-Rule:MF_00354}.
Gene ontology (GO): cytoplasm [GO:0005737]; FMN binding [GO:0010181]; isopentenyl-diphosphate delta-isomerase activity [GO:0004452]; magnesium ion binding [GO:0000287]; NADPH binding [GO:0070402]; oxidoreductase activity [GO:0016491]; isoprenoid biosynthetic process [GO:0008299]
Gene ontology IDs: GO:0000287; GO:0004452; GO:0005737; GO:0008299; GO:0010181; GO:0016491; GO:0070402
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IPP isomerase type 2 family. {ECO:0000256|HAMAP-Rule:MF_00354}.
Protein families: IPP isomerase type 2 family
Coiled coil:
Domain [FT]: DOMAIN 161 317 FMN_dh. {ECO:0000259|Pfam:PF01070}.
Motif:
Region: REGION 5 6 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00354}.
EMBL: CP000896
ProteinModelPortal: A9NGD2
MEROPS:
EnsemblBacteria KO: ABX81412
UniPathway: K01823
CDD:
Gene3D:
HAMAP: 3.20.20.70
InterPro: MF_00354
PANTHER: IPR013785;IPR000262;IPR011179
PIRSF: PTHR10578:SF3
PRINTS: PIRSF003314
PROSITE:
Pfam:
ProDom: PF01070
SMART:
SUPFAM:
TIGRFAMs:

833923-834895(-)

>nucleotide sequence
TTACACTATGTTGTATGTGACTTGTTTTAAATCTTTAAGACTAGAAACGCCAAGAATCAA
CATGATTTTTTTAAGATCTTCAATGAATTCCTCGACCTTTTTAACTGCGGCAGCAAATTC
TAAATCCGTTAAGTCTAAGAACCATTTAGATAGTCCACAAGCTTTAGCACCCAAGATGAG
CGATTTAATAACATCTAGTGGATGACGTATACCACCTGATGCATATATTTCTAAATCTTT
TTCATTTTTAAGATTGATTAAGGCTTGAGCAGTATCAATTGAAAAATCATTTAAGTAACT
ATAGTCCTTAGCACTTCTAGTGGATTCGATATCAATAAATGATGTACCACCACTACCTGA
AACATCAATATATTTTACACCTAGTGTTTTTATCTTTTGGATTGTCTTTAGCGATAAACC
TAGTCCAACTTGTTTAACAATAACCGGTTTATTCAAATGCTTAACGACAGATTCAATGTG
GTTAGACCACAATCTAAAATCTCTGTCACCTTCATTCATAACCAGTTCTTGAATCACATT
TAAGTGAATTGATAAAGCATTAGCTTGGATTGTAGAAACTGCTACTTGTGCTTGTTCTAG
TGTCATATTAGGGTTTATATTACCGACTATAATGCCTTTATGATTATTTCTTATCACTTT
AAATGAATCAATTGAAGATGGATCTTTAAACATAATCGATTGACTTCCAGTCACCATCGG
TAAATTAAAATGATCTGCAATCTTTGATAAAAATTCATTAATCTTATGTGCTTTTTCACT
ACCGCCAGTCATTGCATTAATATAGAACGGATACGGAACCTTCATACCTAAAAATTCTGT
AGATAAATCAATATCGTCCATAGATAAATCAGGTAAATCTGTACCCTCTAAAAGTATCTT
ATCAAACATATTTGATTTTTTCTTGAATGACTTTGCAATATTAATATGATCGTCTTTTCT
ATTTTTACTCAT

>protein sequence
MSKNRKDDHINIAKSFKKKSNMFDKILLEGTDLPDLSMDDIDLSTEFLGMKVPYPFYINA
MTGGSEKAHKINEFLSKIADHFNLPMVTGSQSIMFKDPSSIDSFKVIRNNHKGIIVGNIN
PNMTLEQAQVAVSTIQANALSIHLNVIQELVMNEGDRDFRLWSNHIESVVKHLNKPVIVK
QVGLGLSLKTIQKIKTLGVKYIDVSGSGGTSFIDIESTRSAKDYSYLNDFSIDTAQALIN
LKNEKDLEIYASGGIRHPLDVIKSLILGAKACGLSKWFLDLTDLEFAAAVKKVEEFIEDL
KKIMLILGVSSLKDLKQVTYNIV