ACL_RS03980

Uniprot: A9NGD7
Description: pseudouridine-5'-phosphate glycosidase
EC number: 4.2.1.70
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H(2)O. {ECO:0000255|HAMAP-Rule:MF_01876}.
Cofactor: COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01876}; ; Note=Binds 1 Mn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01876}
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway. {ECO:0000255|HAMAP-Rule:MF_01876}.
Pathway:
Active site: ACT_SITE 25 25 Proton donor. {ECO:0000255|HAMAP-Rule:MF_01876}.; ACT_SITE 159 159 Nucleophile. {ECO:0000255|HAMAP-Rule:MF_01876}.
Binding site: BINDING 86 86 Substrate. {ECO:0000255|HAMAP-Rule:MF_01876}.; BINDING 106 106 Substrate; via amide nitrogen. {ECO:0000255|HAMAP-Rule:MF_01876}.
Calcium binding:
DNA binding:
Metal binding: METAL 138 138 Manganese. {ECO:0000255|HAMAP-Rule:MF_01876}.
Nucleotide binding:
Site:
Gene names (primary): psuG
Gene names (synonym): indA
Mass: 32,480
Subunit structure [CC]: SUBUNIT: Homotrimer. {ECO:0000255|HAMAP-Rule:MF_01876}.
Gene ontology (GO): hydrolase activity, acting on glycosyl bonds [GO:0016798]; metal ion binding [GO:0046872]; pseudouridylate synthase activity [GO:0004730]; nucleobase catabolic process [GO:0046113]
Gene ontology IDs: GO:0004730; GO:0016798; GO:0046113; GO:0046872
Chain: CHAIN 1 303 Pseudouridine-5'-phosphate glycosidase. /FTId=PRO_0000390506.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the pseudouridine-5'-phosphate glycosidase family. {ECO:0000255|HAMAP-Rule:MF_01876}.
Protein families: Pseudouridine-5'-phosphate glycosidase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 140 142 Substrate binding. {ECO:0000255|HAMAP-Rule:MF_01876}.
EMBL: CP000896
ProteinModelPortal: A9NGD7
MEROPS:
EnsemblBacteria KO: ABX81417
UniPathway: K16329
CDD:
Gene3D:
HAMAP:
InterPro: MF_01876
PANTHER: IPR007342
PIRSF:
PRINTS:
PROSITE:
Pfam:
ProDom: PF04227
SMART:
SUPFAM:
TIGRFAMs:

838412-839324(+)

>nucleotide sequence
ATGAACCAATTCATTGATATTCATCCAAAAGTGTTAGATGCCCTAAGATCAGGTAAGCCT
GTTGTTGCGTTAGAATCTACGATTATTGCACATGGTATGCCTTATCCTAAAAATGTAGAA
ACGGCACTCAATGTTGAAAAAATAGTTGAACAAAATGGTGCTATACCTGCGACAATTGCG
ATTATAGATGGTCGTATTAAAGTGGGCTTAAATCCAAATGAACTTACATCGTTAGGAAAA
CTTTCTAATGTACTCAAAGTATCCAAACGCGATATACCTTATTGTGTTATTAAAAAATAC
AGTGGTGCTACTACCGTTTCAGCCACCATCCTTATTGCTGATATGGTAGGTATCAAAGTA
TTTGCAACAGGTGGTATCGGTGGTGTACATAAGGATGCTGCTGCTACATTTGATATTTCA
AGAGATCTTGAAGAAATTTCTGAAAAACAGGTCGCAGTTGTCTCAGCTGGGGCTAAAGCC
ATACTAGACTTAGATCTTACACTAGAATATCTTGAGACCAAGGGTGTTGAAGTAATCGGA
TATAAAACAAGTGAATTTCCTGCATTCTTTTCAAATGCATCTGGTTTAAATACTACTTTT
CAATTAGATGAGGCAAGTCAAATTGCACAACTCATGTATACAAAATGGAATATGGGTTTA
AGTGGTGGTATCGTCGTTGCAAATCCAATACCTCAAAATTACGCAATAGATAAAGACAGT
TTAGATAAAGCAATTTCAAGTGCTATATTATCAGCTAAAGATAATAAAATTACTGGTAAA
GAATTAACACCATACTTGCTTAAAATGCTACATAGTAACAAGGATATAACAAGTCTTGAA
TCTAATATTGGTCTTATCTACAATAATGCGAAAATTGCAGCATTGATTGCTGTTGAATTT
TCTAAACTTTAG

>protein sequence
MNQFIDIHPKVLDALRSGKPVVALESTIIAHGMPYPKNVETALNVEKIVEQNGAIPATIA
IIDGRIKVGLNPNELTSLGKLSNVLKVSKRDIPYCVIKKYSGATTVSATILIADMVGIKV
FATGGIGGVHKDAAATFDISRDLEEISEKQVAVVSAGAKAILDLDLTLEYLETKGVEVIG
YKTSEFPAFFSNASGLNTTFQLDEASQIAQLMYTKWNMGLSGGIVVANPIPQNYAIDKDS
LDKAISSAILSAKDNKITGKELTPYLLKMLHSNKDITSLESNIGLIYNNAKIAALIAVEF
SKL