ACL_RS04000

Uniprot: A9NGE2
Description: polyribonucleotide nucleotidyltransferase
EC number: 2.7.7.8
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. {ECO:0000255|HAMAP-Rule:MF_01595}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01595};
Enzyme regulation:
Function [CC]: FUNCTION: Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction. {ECO:0000255|HAMAP-Rule:MF_01595}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 500 500 Magnesium. {ECO:0000255|HAMAP-Rule:MF_01595}.; METAL 506 506 Magnesium. {ECO:0000255|HAMAP-Rule:MF_01595}.
Nucleotide binding:
Site:
Gene names (primary): pnp
Gene names (synonym):
Mass: 78,666
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; 3'-5'-exoribonuclease activity [GO:0000175]; magnesium ion binding [GO:0000287]; polyribonucleotide nucleotidyltransferase activity [GO:0004654]; RNA binding [GO:0003723]; mRNA catabolic process [GO:0006402]; RNA processing [GO:0006396]
Gene ontology IDs: GO:0000175; GO:0000287; GO:0003723; GO:0004654; GO:0005737; GO:0006396; GO:0006402
Chain: CHAIN 1 715 Polyribonucleotide nucleotidyltransferase. /FTId=PRO_0000329476.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the polyribonucleotide nucleotidyltransferase family. {ECO:0000255|HAMAP-Rule:MF_01595}.; SIMILARITY: Contains 1 KH domain. {ECO:0000255|HAMAP-Rule:MF_01595}.; SIMILARITY: Contains 1 S1 motif domain. {ECO:0000255|HAMAP-Rule:MF_01595}.
Protein families: Polyribonucleotide nucleotidyltransferase family
Coiled coil:
Domain [FT]: DOMAIN 567 634 KH. {ECO:0000255|HAMAP-Rule:MF_01595}.; DOMAIN 637 712 S1 motif. {ECO:0000255|HAMAP-Rule:MF_01595}.
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGE2
MEROPS:
EnsemblBacteria KO: ABX81422
UniPathway: K00962
CDD:
Gene3D:
HAMAP: 1.10.10.400;2.40.50.140;3.30.1370.10;3.30.230.70
InterPro: MF_01595
PANTHER: IPR001247;IPR015847;IPR004087;IPR004088;IPR012340;IPR012162;IPR027408;IPR015848;IPR020568;IPR022967;IPR003029
PIRSF: PTHR11252
PRINTS: PIRSF005499
PROSITE:
Pfam: PS50126
ProDom: PF00013;PF03726;PF01138;PF03725;PF00575
SMART:
SUPFAM: SM00322;SM00316
TIGRFAMs: SSF46915;SSF50249;SSF54211;SSF54791;SSF55666

843934-846082(-)

>nucleotide sequence
TTATAAAGCATCTTTTCTTGATAAGTCAACACGTCCACGTTCATCAATCTTAACAACTTT
AACTAAGATTTGATCACCAACTTTAACTTTACCATCTAAAGATTCTGTTCTTTCTTTTTC
CCATTTAGAAATGTGGACTAATCCTTCAGTTCCTGGGAAGATTTCAGCAAATACACCTTG
AATTTTCTTACCATCTTTATCCATTAATAAGCGTGTTACTTTAGCTTCATATAGTTCGCC
AACTTTAGCTTCTCTTACTAAACTTTCGATGTAAGCAGCAGTTTTGTTTAACCATGCAGA
GTCAGTATGCATAATGAATACTCTACCATCTTGTTCAATATCAATTTTAACGTTGTTATG
ATCTTCAATGATTTGAGTAATGATCTTACCACCAGCACCGATAACGTCTCTAATCTTATC
TGGATTAATACGAATCATCTTAACTTTAGGTGCGAATGCACTTAATTCAGTTCTTGTTTC
ACTAATTACAGTGTTCATGTGTGAAAGAATATGAAGTCTACCCTTACGAGCTTGTTCTAA
TGCTTCTTTCATAATCTCAGTTGTAATACCTTGAATTTTGATATCCATTTGAAGTGCAGT
AATACCATCTTTAGTACCTGCAACTTTAAAGTCCATATCGCCTTCATGGTCTTCCATACC
TTGAATATCACTTAAAATTGAGTAATGTTCACCGTCCATGATTAAGCCCATTGCGATACC
TGCAACTGGCGCTTTAATTGGAACACCAGCAGCCATTAAAGCCATAGAGCCTACACAAAT
TGTAGCTTGAGAACTTGAACCGTTAGATTCAGTAATTTCAGATACTACACGGATTGCATA
TGGGAATTCATCTTCAGAAGGTAATACTTGTAATAAAGCACGTTCTCCTAAAGCACCGTG
ACCAATTTCACGACGTCCAGGTGCACCATAACGACCTGTTTCACCTACTGAAAATGGTGG
GAAGTTATAATGTAACATGAAGCGTTTTGTAACTGTTTCTTGTTCTAAACCATCAATAAT
TTGATTTTCATTTAATGAACCTAATGTGACTATACCTAAAGCTTGAGTTTGACCTCTTGT
AAATAGTGCAGAACCATGAGTTCTTGGTAATAAATCAACTGAGGATGCAAGTGGTCTAAT
TTCATCTAAACCACGGCCATCAGGTCTTACTTTATCTTCAGTAATAAGGCGTCTAACTTC
TTTAGTTACAATTCTGTCTAAAGAAACTTTAACTTGATTCATTAATTCTTTCTTTTGATC
AGCATCTAGCACTTCTTTACCATCTACTGTCATCCAGAAATTTCTTTGTTCAAAGTGCGC
TACTACTTCGTCTTTGATTGCATCAATTACATCATAACGTTCTTGTTTGTCAACAAGTGA
AACAGCTTTAACTAATTCTTTACCTTTGTAAGCAAATACTTCATCAAAGATTGCTTGATT
AAGACTTAGTAATTCAGGTTCTACTTTTTCAACTCCAAAAGCTTTTTTAATTGTTTCTTG
GAAAGCACATAATTCTTTGATTGCTTCATGACCAAATAAAATAGCTTCTAACATAACTTC
TTCAGATACTTGTTTAGCGCCTGCTTCAACCATGTTAATTGCATCTTTTGTACCTGCAAC
GATTAAATCGATGTCTGATACATTTAATTGTTCTGAAGATGGATTTAATACGAATTGTCC
ATCAATACGACCAACATGTGCACCAGCGATTGGTCCCATGAATGGAATATTTGAAATTTC
AAGTGCAATCGATGAACCGAGCATTGCAGCCATTTGGCTTGATGCTTCAGGATCTGATGA
TAACACTGTATTCACAACTTGAACTTCATTTCTATAACCATCGTCAAATAATGGACGTAA
TGGTCTATCAATTAATCTTGATGTCAATGTTTCAACTTCTGATGGTCTACCTTCTCTTCT
GAAGAATCCACCAGGAATTTTACCTGCAGCATATTGTTTTTCTGCATAAATTACCATAAG
TGGGAAAAAGTCAGTTTGACCCACTTTCGCTTTAGCAACAGCAGTTGATAATACAACAGT
GTCACCATAATAAATCATTGCAGCGCCATTTGCTTGTTTAGCAATTTCGCCGACTTCAAC
TCTTAGTGGTTTCCCAGCAAGTGTTGTTTCAAATACTTGTTTAGACAT

>protein sequence
MSKQVFETTLAGKPLRVEVGEIAKQANGAAMIYYGDTVVLSTAVAKAKVGQTDFFPLMVI
YAEKQYAAGKIPGGFFRREGRPSEVETLTSRLIDRPLRPLFDDGYRNEVQVVNTVLSSDP
EASSQMAAMLGSSIALEISNIPFMGPIAGAHVGRIDGQFVLNPSSEQLNVSDIDLIVAGT
KDAINMVEAGAKQVSEEVMLEAILFGHEAIKELCAFQETIKKAFGVEKVEPELLSLNQAI
FDEVFAYKGKELVKAVSLVDKQERYDVIDAIKDEVVAHFEQRNFWMTVDGKEVLDADQKK
ELMNQVKVSLDRIVTKEVRRLITEDKVRPDGRGLDEIRPLASSVDLLPRTHGSALFTRGQ
TQALGIVTLGSLNENQIIDGLEQETVTKRFMLHYNFPPFSVGETGRYGAPGRREIGHGAL
GERALLQVLPSEDEFPYAIRVVSEITESNGSSSQATICVGSMALMAAGVPIKAPVAGIAM
GLIMDGEHYSILSDIQGMEDHEGDMDFKVAGTKDGITALQMDIKIQGITTEIMKEALEQA
RKGRLHILSHMNTVISETRTELSAFAPKVKMIRINPDKIRDVIGAGGKIITQIIEDHNNV
KIDIEQDGRVFIMHTDSAWLNKTAAYIESLVREAKVGELYEAKVTRLLMDKDGKKIQGVF
AEIFPGTEGLVHISKWEKERTESLDGKVKVGDQILVKVVKIDERGRVDLSRKDAL