ACL_RS04020

Uniprot: A9NGE6
Description: tRNA pseudouridine synthase B
EC number: 5.4.99.25
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: tRNA uridine(55) = tRNA pseudouridine(55). {ECO:0000255|HAMAP-Rule:MF_01080}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-Rule:MF_01080}.
Pathway:
Active site: ACT_SITE 38 38 Nucleophile. {ECO:0000255|HAMAP-Rule:MF_01080}.
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): truB
Gene names (synonym):
Mass: 31,306
Subunit structure [CC]:
Gene ontology (GO): pseudouridine synthase activity [GO:0009982]; RNA binding [GO:0003723]; tRNA pseudouridine synthesis [GO:0031119]
Gene ontology IDs: GO:0003723; GO:0009982; GO:0031119
Chain: CHAIN 1 279 tRNA pseudouridine synthase B. /FTId=PRO_1000084540.
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
Protein families: Pseudouridine synthase TruB family, Type 1 subfamily
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: CP000896
ProteinModelPortal: A9NGE6
MEROPS:
EnsemblBacteria KO: ABX81426
UniPathway: K03177
CDD:
Gene3D:
HAMAP:
InterPro: MF_01080
PANTHER: IPR020103;IPR002501;IPR014780
PIRSF: PTHR13767
PRINTS:
PROSITE:
Pfam:
ProDom: PF01509
SMART:
SUPFAM:
TIGRFAMs: SSF55120

847927-848767(-)

>nucleotide sequence
TTAAAATAGATATTTCAATTTATACTGTTGATTATCAGGTACGTAGTATGCAATGTAGTT
ACCCATTTCATCTTGTACAATAAATGGTTCATTTAAGGTGGTTTGACGTTCATCTAATAC
AACACCATTTTTAACAAGTTTAATTAAGTAGTCATTTAAGACTATTTTCTTAACACCTTC
AAACAATAAAGCGTGATCAATTAAGTCAGGTAGTTCCGTATCTTCAACTGTCTTTGACAT
ATTTTGGCTATATGCATCAATGTTTGTTCTATTTAAGACCGACAGTGCACCAAAGGTATT
TAATCCTAATCCGATATCTCTTGCGATACTTCGAATGTATGTACCCTTAGATACATGTGT
AGAAAAATCTATTGTTTGTTTATTTATTTGATAAGTAAAATTATAAATACTTACTTCTCT
AGCAGGAAGATCTAGTGGCTTACCACTTCTTGCAGCCTCATAGGCTTTAACACCAGCTTT
CTTGATTGCTGAATAGCTAGGAGGTATTTGCTTATAAGTAGGCACTATACGGTTTAAAAT
AGCTTTTAAATCATCTTCTGTAAATGATACAGGTGCTTCATTTATGACTTCGCCTGTGGT
GTCATCTGTATCATATGATTTACCAAGTACAATGGTACCATCATATTGCTTATCAAGCTC
ATCAAATAGAAAGGCTAACTTTGTTGCTTTACCGACTAAAATAATTAATAAACCGGTAGC
AAAAGGATCTAGTGTTCCTGTATGTCCTACTTTTTTAATATTAAATTTCTTTCTAATTTG
ATAAACAATATCATGGCTGGTTAGCCCAGCTTTTTTATTGACTAAAAATATACCGTTCAT

>protein sequence
MNGIFLVNKKAGLTSHDIVYQIRKKFNIKKVGHTGTLDPFATGLLIILVGKATKLAFLFD
ELDKQYDGTIVLGKSYDTDDTTGEVINEAPVSFTEDDLKAILNRIVPTYKQIPPSYSAIK
KAGVKAYEAARSGKPLDLPAREVSIYNFTYQINKQTIDFSTHVSKGTYIRSIARDIGLGL
NTFGALSVLNRTNIDAYSQNMSKTVEDTELPDLIDHALLFEGVKKIVLNDYLIKLVKNGV
VLDERQTTLNEPFIVQDEMGNYIAYYVPDNQQYKLKYLF